CAZyme Information: MGYG000002216_01658

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae
• CAZyme ID: MGYG000002216_01658
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1346	MGYG000002216_49|CGC1	146814.63	6.1494

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002216	3003083	MAG	Spain	Europe

• Gene Location: Start: 2940; End: 6980 Strand: +

Full Sequence      

MQRSTMKKKTLAFVLAASMCMGNILPAVAAPGTEKAEETAMLAHYPLQSNAQDVSGNANH
GTVHGNVSWTDGLVLPGGTKTDAANASYVTLPEGMFDGQDEVTVSVWINSNTDAGNYSAL
FFGTKTQSNNLPLNYWLFNPTNPSGMFKSVFTDANSTDTPWGTEVGVSSVSTTEYKGVWT
HYTTVLTEDSVTGYINGEKIGTAQKTKTTSDFGEDLQAYIGRSNYTQDSTYAGSFQDLRI
YDQAMTQEEVEEVYLDTSSVNDSVKAKALEGIAKRLTLDGVLDMGYVGKDGMLSLPRETN
GATITWSSDRKDIINDNGNVTLPEQTTTVTLTARMSLGTHIVTKDFAVAVLARDGQMEFW
KEHMTIPYVLSQGDVLPVNLGNVKVTWTGDDAIREDGTVVPKFSGKRKVTLKATMTEGQQ
SEEKQFEAYILGEDAVYVESYTRASDGDYSNNLAYSMHLAYSEDGNGGYTPLNDNSGLLF
MRATENADQTLNRKGLKSPYLFYTKEGNFGVAATRVEVNGAMDNQAASSVAFWISDNLRA
YQEVSLLNLKTEHTVNNPVCEYDAGADLYRITWADENGNYYQNTVKDLADPESVSDPVSV
LKPTVRQVDTDIAGATEGNVLPLENSVGTKLKTKLSKLVNTGMKVPEQITAASKEEVEAI
RATAVYNDGSTASKQVKWDLSQVDFTKPGLYTVPGTVTQPSFQNDQPMFVGRPDPEMAKY
NGKFYFISTDENGQSRIYIRQSDTLASIGQSSESLILDGGSYPDLFVNCLWAPELHVIGD
DLYVFFAGSLSGWSGVQSYVMKLKEGGDPTRKSDWERPIRVLDQDGNNLYDTSSGITLDM
TYFEVGGQAYVVWAQRQIHPVDNGSWLYIATVDQEKPWQLTSDRVCISKPDYGWDNNDTF
VDEGPFLIQRNGKLYLTFSGGMVNPTYCVGILTADEKDNLLDASVWTKGNYPILTSRSVE
NQYGPGHNSYIYDDDGTLYNVYHAQWNKGTRSASIRRVHFDIDGDPVLDLVEERDLVDAY
KNVSMKVLVPGEVTVTYTAQEGGRIEGASVQKILMGGSTTAVTAVANPGYEFVGWNDGKT
QAVRSDSNVAETVAYQAIFRKVAEPVPKVTVTYVAGNGGRIAGATVQTIEKGGSAAEVTA
VADPGYSFSKWSDGVTTAKRKDSNVTESKTLVAEFIKNQATETPEASAVKLNVKKKITIG
VKEKVKLKASVLPAGASQKIEWKSSKKSVVSVAANGKITGRKAGTAIITATTENGKKVTC
RVTVKKAPKKITTKVTAKTLKKGRTWKMKVSFPKKTASYRVTFTSSKKKVATVNANGKIQ
ARKKGTAVITARSYNGKKARVRITVK

Enzyme Prediction     

EC	3.2.1.55	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH43	713	1006	6e-103	0.9933110367892977

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd18818	GH43_GbtXyl43B-like	2.59e-150	714	985	2	273	Glycosyl hydrolase family 43 such as Geobacillus thermoleovorans IT-08 beta-xylosidase/exo-xylanase (GbtXyl43B). This glycosyl hydrolase family 43 (GH43) subgroup includes the characterized enzymes Geobacillus thermoleovorans IT-08 beta-xylosidase (EC 3.2.1.37) / exo-xylanase (GbtXyl43B), and Paenibacillus sp. strain E18 alpha-L-arabinofuranosidase (EC 3.2.1.55) Abf43B. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18819	GH43_LbAraf43-like	1.18e-77	721	984	11	276	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08980	GH43_LbAraf43-like	7.25e-77	714	984	2	275	Glycosyl hydrolase family 43 proteins such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. In addition to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) familiesGH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18820	GH43_LbAraf43-like	7.42e-56	713	984	1	257	Glycosyl hydrolase family 43 proteins similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans GbtXyl43B. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes enzymes with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities, similar to Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43 and Geobacillus thermoleovorans IT-08 beta-xylosidase / exo-xylanase (GbtXyl43B). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18817	GH43f_LbAraf43-like	1.78e-54	714	984	2	261	Glycosyl hydrolase family 43 such as Lactobacillus brevis alpha-L-arabinofuranosidase LbAraf43. This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with alpha-L-arabinofuranosidase (EC 3.2.1.55) activity. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF28727.1	7.89e-263	8	1029	4	1015
BBF42300.1	1.13e-247	7	1346	5	1378
AOZ92647.1	1.46e-195	433	1035	10	611
ANY68655.1	2.84e-185	434	1029	8	603
AGF58614.1	6.76e-184	431	1029	5	603

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3K1U_A	1.84e-41	707	1009	12	294	Beta-xylosidase,family 43 glycosyl hydrolase from Clostridium acetobutylicum [Clostridium acetobutylicum]
3AKF_A	4.04e-39	700	1006	7	310	Crystalstructure of exo-1,5-alpha-L-arabinofuranosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKG_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranobiose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKH_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-1,5-L-arabinofuranotriose [Streptomyces avermitilis MA-4680 = NBRC 14893],3AKI_A Crystal structure of exo-1,5-alpha-L-arabinofuranosidase complexed with alpha-L-arabinofuranosyl azido [Streptomyces avermitilis MA-4680 = NBRC 14893]
5M8B_A	1.20e-38	707	1009	30	317	ChainA, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis],5M8B_B Chain B, Alpha-L-arabinofuranosidase II [Levilactobacillus brevis]
5M8E_A	7.12e-38	705	1009	26	315	Crystalstructure of a GH43 arabonofuranosidase from Weissella sp. strain 142 [Weissella cibaria],5M8E_B Crystal structure of a GH43 arabonofuranosidase from Weissella sp. strain 142 [Weissella cibaria]
4QVS_A	3.81e-07	52	261	23	227	ChainA, S-layer domain-containing protein [Acetivibrio thermocellus ATCC 27405]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G4MMH2	1.96e-43	664	1006	6	342	Alpha-L-arabinofuranosidase B OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=abfB PE=1 SV=1
P82594	2.82e-41	705	988	51	322	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces chartreusis OX=1969 PE=1 SV=1
Q82P90	2.07e-38	688	1006	24	336	Extracellular exo-alpha-(1->5)-L-arabinofuranosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=Araf43A PE=1 SV=1
A0A3R0A696	2.82e-06	1193	1265	278	352	Alpha-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=blArafA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000344	0.998674	0.000362	0.000223	0.000194	0.000164

TMHMM  Annotations     

start	end
11	33