CAZyme Information: MGYG000002230_00927

Basic Information

• Species: Ruminococcus sp900545125
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus; Ruminococcus sp900545125
• CAZyme ID: MGYG000002230_00927
• CAZy Family: CBM35
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
637		70891.95	4.1396

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002230	2337720	MAG	United States	North America

• Gene Location: Start: 10167; End: 12080 Strand: +

Full Sequence      

MICPEFTDRSAMEVNIMKKSIKNPLAASLAAVVLMTGTAFSQTAPEKIPVSAETGVMKYE
FEEGSTSGGKIHTEGWKGNTKEDGSGEEMDLTSFSGSGFSYLDQKGTTVSVEVDVPEAGL
YEMNICYCEPYDTNKKVQYLQVNGVNQGELSFIHNLKFEETSGGVVNLKKGKNTIELKGY
WGYTFFDYLTLTPADEKLSNLSPVRTLSNPNASDSAKRLYSYLCDQYGKHIIAGQQEYCG
EHNYNLWSDPNTFIKDNEAEFEYILDKTGKQPAIRGIDLLAYRSNTNWTDHAPERAIEWV
NEYKGIATLTWHWNVPSTEDGGDDVAFYVESTGNTPFTTFSITNALEEGTWENKQLLADI
DAIAAELTKLKEADVPVLWRPLHEAEGGWFWWGAEGAEPCKKLYRLLYDKLTNEYGLDNL
IWVWTGYAYPTSADWYPGDDVVDIIGYDKYNAVDGLPNLSSIASTFYSLVQSTDGQKMVA
MSENDSIPSLENLLNDKAGWLYFCPWYMNYLTSEQNNPVDSLVEIYNSDYCITLDELPDL
KSYPINGSDDPIIPPPDIKKTVPGDADCDGDVRINDAVLIMQAIGNPDAYGVSGSEKSHI
TKQGYINADVTGDGDGLTNLDALEIQKYLLNLVSELK

Enzyme Prediction     

EC	3.2.1.78

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH26	208	536	3.8e-110	0.9966996699669967
CBM35	89	191	2.3e-23	0.8739495798319328

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02156	Glyco_hydro_26	1.43e-59	209	536	2	311	Glycosyl hydrolase family 26.
cd04086	CBM35_mannanase-like	1.14e-32	58	192	2	119	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
COG4124	ManB2	2.86e-19	335	456	139	267	Beta-mannanase [Carbohydrate transport and metabolism].
pfam16990	CBM_35	9.06e-09	59	193	2	119	Carbohydrate binding module (family 35). This is a mannan-specific carbohydrate binding domain, previously known as the X4 module. Unlike other carbohydrate binding modules, binding to substrate causes a conformational change.
cd04083	CBM35_Lmo2446-like	2.50e-08	58	191	2	125	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADU21882.1	1.11e-305	17	636	1	601
CBL18146.1	2.11e-221	50	593	29	576
AEV68433.1	1.07e-209	57	585	36	543
BAB19050.1	2.13e-207	58	584	37	545
ALX07411.1	4.28e-207	58	584	37	545

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZM8_A	2.66e-82	86	537	24	447	ChainA, Gh26 Endo-beta-1,4-mannanase [Podospora anserina S mat+]
6HPF_A	6.31e-59	202	537	1	310	Structureof Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose [Yunnania penicillata]
3WDQ_A	2.12e-57	210	538	37	353	Crystalstructure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus [Symbiotic protist of Reticulitermes speratus],3WDR_A Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus complexed with gluco-manno-oligosaccharide [Symbiotic protist of Reticulitermes speratus]
6HF2_A	5.68e-51	193	519	31	327	Thestructure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus [Bacteroides ovatus ATCC 8483],6HF4_A The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus, complexed with G1M4 [Bacteroides ovatus ATCC 8483]
6D2X_A	5.83e-45	207	537	4	334	Crystalstructure of the GH26 domain from PbGH26-GH5A endo-beta-mannanase/endo-beta-glucanase from Prevotella bryantii [Prevotella bryantii B14]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P55297	5.51e-84	88	538	38	462	Mannan endo-1,4-beta-mannosidase B OS=Piromyces sp. OX=45796 GN=MANB PE=2 SV=1
P55298	2.12e-82	79	538	27	460	Mannan endo-1,4-beta-mannosidase C OS=Piromyces sp. OX=45796 GN=MANC PE=2 SV=1
G2Q4H7	9.52e-81	85	537	52	476	Mannan endo-1,4-beta-mannosidase OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=Man26A PE=1 SV=1
P55296	8.23e-79	90	538	39	461	Mannan endo-1,4-beta-mannosidase A OS=Piromyces sp. OX=45796 GN=MANA PE=2 SV=1
P49425	1.87e-69	88	548	32	468	Mannan endo-1,4-beta-mannosidase OS=Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) OX=518766 GN=manA PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.005147	0.993461	0.000673	0.000294	0.000215	0.000193

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002230_00927.