CAZyme Information: MGYG000002232_01682

Basic Information

• Species: Eubacterium_R sp900539845
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Eubacterium_R; Eubacterium_R sp900539845
• CAZyme ID: MGYG000002232_01682
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
546		62322.28	6.459

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002232	2282853	MAG	United States	North America

• Gene Location: Start: 66273; End: 67913 Strand: +

Full Sequence      

MREKIVLSQWDFSIEKLHYRAKVQVPHTWNVCDETQNYRGRADYSTTVFIDEKMKDKTVT
LCFGAVYHTAYVTVNGAFIGQHSGSGYTPFSFDITKAVRFGAENTIEVTADNRPCRNMLP
YLKNFDWADDGGLIREVSLLFADHDSIRRMQVSAQITDISGDLCSGYLGLDIAFFDGHKN
DQVKLSVSDSKTGEVVLSCDKAIRSNRAEIHFTDLKLWNTDSPNLYHITAETSDDSFTQR
IGLRKIKINGNRIFLNNKAVYLTGCEWMPGSHPDHGMAEPAEHSIMRLKQLKDSGCIFTR
FHWQQDSSIYDWCDENGLLVQEEIPYWGSPKKAAKKQLEIAKLHADEMLEAHFNHPSIIF
WGVGNELGGLFESTKKYVREMVAYFKSKDQTRCVNYVSNTLGFVKGKKDEATLYGDVAMW
NEYLGLWHPCNDIPGKILEVNALCKDKPLVITEFGLCEPHFSGGDERRTRILVDNVDFFN
DADNIQGYVWFSLNDYRTHVGEQGEGRLKQRVHGSTDLYGNKKPSYDVLCEINKKQTERF
RGVYGD

Enzyme Prediction     

No EC number prediction in MGYG000002232_01682.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	9	503	7.1e-83	0.5718085106382979

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	7.54e-53	1	433	9	485	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.09e-41	25	455	47	510	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.86e-32	44	490	113	543	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	4.83e-14	246	490	1	240	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
pfam02837	Glyco_hydro_2_N	3.82e-13	11	140	39	166	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QHQ59621.1	8.13e-144	2	526	3	545
ASA20751.1	1.57e-135	21	533	32	551
QBE94721.1	1.79e-134	2	526	3	548
AIQ51262.1	2.66e-131	23	531	15	531
AIQ39960.1	4.63e-131	23	533	44	562

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ECA_A	4.68e-36	8	523	60	597	Lactobacillusrhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus],6ECA_B Lactobacillus rhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus]
6NCY_A	5.56e-36	23	540	87	598	Crystalstructure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCY_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans [Fusicatenibacter saccharivorans],6NCZ_A Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_B Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_C Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_D Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_E Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans],6NCZ_F Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans bound to phenyl-thio-beta-D-glucuronide [Fusicatenibacter saccharivorans]
3CMG_A	6.78e-35	1	428	3	449	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
5Z1A_A	7.26e-35	1	428	22	468	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
6D89_A	1.03e-34	3	541	66	595	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26257	1.30e-35	13	519	23	531	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
T2KPJ7	2.83e-32	23	367	82	431	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	1.17e-26	4	363	24	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
P24131	4.38e-24	23	436	98	519	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2
Q56307	8.78e-24	44	454	114	508	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000046	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002232_01682.