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CAZyme Information: MGYG000002251_00065
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Basic Information |
Genomic context |
Full Sequence |
Enzyme annotations |
CAZy signature domains |
CDD domains |
CAZyme hits |
PDB hits |
Swiss-Prot hits |
SignalP and Lipop annotations |
TMHMM annotations
Basic Information help
| Species | RQCD01 sp008668455 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lineage | Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; RQCD01; RQCD01 sp008668455 | |||||||||||
| CAZyme ID | MGYG000002251_00065 | |||||||||||
| CAZy Family | GH38 | |||||||||||
| CAZyme Description | Mannosylglycerate hydrolase | |||||||||||
| CAZyme Property |
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| Genome Property |
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| Gene Location | Start: 2850; End: 5168 Strand: - | |||||||||||
CAZyme Signature Domains help
| Family | Start | End | Evalue | family coverage |
|---|---|---|---|---|
| GH38 | 3 | 259 | 3.2e-54 | 0.9925650557620818 |
CDD Domains download full data without filtering help
| Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
|---|---|---|---|---|---|---|---|
| cd10789 | GH38N_AMII_ER_cytosolic | 5.84e-83 | 3 | 239 | 1 | 248 | N-terminal catalytic domain of endoplasmic reticulum(ER)/cytosolic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The subfamily is represented by Saccharomyces cerevisiae vacuolar alpha-mannosidase Ams1, rat ER/cytosolic alpha-mannosidase Man2C1, and similar proteins. Members in this family share high sequence similarity. None of them have any classical signal sequence or membrane spanning domains, which are typical of sorting or targeting signals. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 utilizes both the cytoplasm to vacuole targeting (Cvt, nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Man2C1is involved in oligosaccharide catabolism in both the ER and cytosol. It can catalyze the cobalt-dependent cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl-enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst. |
| pfam01074 | Glyco_hydro_38 | 1.08e-50 | 3 | 260 | 1 | 271 | Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. |
| COG0383 | AMS1 | 9.81e-48 | 3 | 772 | 199 | 922 | Alpha-mannosidase [Carbohydrate transport and metabolism]. |
| cd10813 | GH38N_AMII_Man2C1 | 5.09e-36 | 4 | 239 | 2 | 248 | N-terminal catalytic domain of mammalian cytosolic alpha-mannosidase Man2C1 and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily corresponds to cytosolic alpha-mannosidase Man2C1 (also known as ER-mannosidase II or neutral/cytosolic mannosidase), mainly found in various vertebrates, and similar proteins. Man2C1 plays an essential role in the catabolism of cytosolic free oligomannosides derived from dolichol intermediates and the degradation of newly synthesized glycoproteins in ER or cytosol. It can catalyze the cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Man2C1 is a cobalt-dependent enzyme belonging to alpha-mannosidase class II. It has a neutral pH optimum and is strongly inhitibed by furanose analogs swainsonine (SW) and 1,4-dideoxy-1,4-imino-D-mannitol (DIM), moderately by deoxymannojirimycin (DMM), but not by kifunensine (KIF). DMM and KIF, both pyranose analogs, are normally known to inhibit class I alpha-mannosidase. |
| cd10786 | GH38N_AMII_like | 1.50e-34 | 3 | 170 | 1 | 170 | N-terminal catalytic domain of class II alpha-mannosidases and similar proteins; glycoside hydrolase family 38 (GH38). Alpha-mannosidases (EC 3.2.1.24) are extensively found in eukaryotes and play important roles in the processing of newly formed N-glycans and in degradation of mature glycoproteins. A deficiency of this enzyme causes the lysosomal storage disease alpha-mannosidosis. Many bacterial and archaeal species also possess putative alpha-mannosidases, but their activity and specificity is largely unknown. Based on different functional characteristics and sequence homology, alpha-mannosidases have been organized into two classes (class I, belonging to glycoside hydrolase family 47, and class II, belonging to glycoside hydrolase family 38). Members of this family corresponds to class II alpha-mannosidases (alphaMII), which contain intermediate Golgi alpha-mannosidases II, acidic lysosomal alpha-mannosidases, animal sperm and epididymal alpha -mannosidases, neutral ER/cytosolic alpha-mannosidases, and some putative prokaryotic alpha-mannosidases. AlphaMII possess a-1,3, a-1,6, and a-1,2 hydrolytic activity, and catalyzes the degradation of N-linked oligosaccharides. The N-terminal catalytic domain of alphaMII adopts a structure consisting of parallel 7-stranded beta/alpha barrel. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst. |
CAZyme Hits help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
|---|---|---|---|---|---|
| QIZ10335.1 | 2.11e-148 | 4 | 737 | 21 | 784 |
| AYB43569.1 | 7.01e-148 | 3 | 724 | 6 | 756 |
| QGH33633.1 | 2.01e-147 | 4 | 768 | 9 | 812 |
| QHW33669.1 | 2.40e-147 | 3 | 768 | 6 | 811 |
| AYQ72193.1 | 3.28e-147 | 3 | 768 | 5 | 805 |
PDB Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| 5JM0_A | 1.67e-26 | 3 | 319 | 303 | 651 | Structureof the S. cerevisiae alpha-mannosidase 1 [Saccharomyces cerevisiae S288C] |
| 1HWW_A | 5.00e-06 | 3 | 163 | 52 | 211 | GOLGIALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE [Drosophila melanogaster],1HXK_A Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin [Drosophila melanogaster] |
| 6RPC_A | 5.02e-06 | 3 | 163 | 70 | 229 | ChainA, Alpha-mannosidase 2 [Drosophila melanogaster],6RQZ_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRH_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRJ_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRN_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRU_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRW_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRX_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RRY_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster],6RS0_A Chain A, Alpha-mannosidase 2 [Drosophila melanogaster] |
| 1PS3_A | 5.03e-06 | 3 | 163 | 82 | 241 | Golgialpha-mannosidase II in complex with kifunensine [Drosophila melanogaster] |
| 1QWN_A | 5.03e-06 | 3 | 163 | 82 | 241 | GOLGIALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride [Drosophila melanogaster],1R33_A Golgi alpha-mannosidase II complex with 5-thio-D-mannopyranosylamine [Drosophila melanogaster],1R34_A Golgi alpha-mannosidase II complex with 5-thio-D-mannopyranosylamidinium salt [Drosophila melanogaster],1TQS_A Golgi alpha-Mannosidase II In Complex With Salacinol [Drosophila melanogaster],1TQT_A Golgi alpha-Mannosidase II In Complex With A Diastereomer of Salacinol [Drosophila melanogaster],1TQU_A Golgi alpha-Mannosidase II In Complex With The Salacinol Analog Ghavamiol [Drosophila melanogaster],1TQV_A Golgi alpha-Mannosidase II In Complex With Seleno-Salacinol (Blintol) [Drosophila melanogaster],1TQW_A Golgi alpha-Mannosidase II In Complex With A Diastereomer of Seleno-Salacinol [Drosophila melanogaster],2ALW_A Golgi alpha-mannosidase II complex with Noeuromycin [Drosophila melanogaster],2F18_A GOLGI ALPHA-MANNOSIDASE II complex with (2R,3R,4S)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol [Drosophila melanogaster],2F1A_A GOLGI ALPHA-MANNOSIDASE II COMPLEX WITH (2R,3R,4S)-2-({[(1S)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol [Drosophila melanogaster],2F1B_A GOLGI ALPHA-MANNOSIDASE II COMPLEX WITH (2R,3R,4S,5R)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)-5-methylpyrrolidine-3,4-diol [Drosophila melanogaster],2F7O_A Golgi alpha-mannosidase II complex with mannostatin A [Drosophila melanogaster],2F7P_A Golgi alpha-mannosidase II complex with benzyl-mannostatin A [Drosophila melanogaster],2F7Q_A Golgi alpha-mannosidase II complex with aminocyclopentitetrol [Drosophila melanogaster],2F7R_A Golgi alpha-mannosidase II complex with benzyl-aminocyclopentitetrol [Drosophila melanogaster],2FYV_A Golgi alpha-mannosidase II complex with an amino-salacinol carboxylate analog [Drosophila melanogaster],2OW6_A Golgi alpha-mannosidase II complex with (1r,5s,6s,7r,8s)-1-thioniabicyclo[4.3.0]nonan-5,7,8-triol chloride [Drosophila melanogaster],2OW7_A Golgi alpha-mannosidase II complex with (1R,6S,7R,8S)-1-thioniabicyclo[4.3.0]nonan-7,8-diol chloride [Drosophila melanogaster],3BLB_A Crystal structure of Golgi Mannosidase II in complex with swainsonine at 1.3 Angstrom resolution [Drosophila melanogaster],3BUB_A Golgi alpha-mannosidase II with an empty active site [Drosophila melanogaster],3D4Y_A GOLGI MANNOSIDASE II complex with mannoimidazole [Drosophila melanogaster],3D4Z_A GOLGI MANNOSIDASE II complex with gluco-imidazole [unidentified],3D50_A GOLGI MANNOSIDASE II complex with N-octyl-6-epi-valienamine [Drosophila melanogaster],3D51_A GOLGI MANNOSIDASE II complex with gluco-hydroxyiminolactam [Drosophila melanogaster],3D52_A GOLGI MANNOSIDASE II complex with an N-aryl carbamate derivative of gluco-hydroxyiminolactam [Drosophila melanogaster],3DDF_A GOLGI MANNOSIDASE II complex with (3R,4R,5R)-3,4-Dihydroxy-5-({[(1R)-2-hydroxy-1 phenylethyl]amino}methyl) pyrrolidin-2-one [Drosophila melanogaster],3DDG_A GOLGI MANNOSIDASE II complex with (3R,4R,5R)-3,4-Dihydroxy-5-({[(1R)-2-hydroxy-1 phenylethyl]amino}methyl) methylpyrrolidin-2-one [Drosophila melanogaster],3DX0_A Golgi alpha-Mannosidase II in complex with Mannostatin A at pH 5.75 [Drosophila melanogaster],3DX1_A Golgi alpha-Mannosidase II in complex with Mannostatin analog (1S,2S,3R,4R)-4-aminocyclopentane-1,2,3-triol [Drosophila melanogaster],3DX2_A Golgi mannosidase II complex with MANNOSTATIN B [Drosophila melanogaster],3DX4_A Golgi alpha-Mannosidase II in complex with Mannostatin analog (1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol [Drosophila melanogaster],3EJP_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog: (5R)-5-[2'-oxo-2'-(phenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJQ_A Golgi alpha-Mannosidase II in complex with 5-substitued swainsonine analog: (5R)-5-[2'-oxo-2'-(4-methylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJR_A Golgi alpha-Mannosidase II in complex with 5-substitued swainsonine analog: (5R)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJS_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog: (5S)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJT_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog:(5R)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJU_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog:(5S)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster] |
Swiss-Prot Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| Q54K67 | 3.56e-38 | 3 | 744 | 256 | 1045 | Alpha-mannosidase G OS=Dictyostelium discoideum OX=44689 GN=manG PE=1 SV=1 |
| P21139 | 2.76e-29 | 2 | 337 | 250 | 606 | Alpha-mannosidase 2C1 OS=Rattus norvegicus OX=10116 GN=Man2c1 PE=1 SV=1 |
| Q9NTJ4 | 4.83e-29 | 2 | 333 | 251 | 609 | Alpha-mannosidase 2C1 OS=Homo sapiens OX=9606 GN=MAN2C1 PE=1 SV=1 |
| Q91W89 | 6.37e-29 | 2 | 364 | 250 | 631 | Alpha-mannosidase 2C1 OS=Mus musculus OX=10090 GN=Man2c1 PE=1 SV=1 |
| P22855 | 9.07e-26 | 3 | 319 | 290 | 638 | Alpha-mannosidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AMS1 PE=1 SV=2 |
SignalP and Lipop Annotations help
This protein is predicted as OTHER
| Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
|---|---|---|---|---|---|
| 1.000055 | 0.000000 | 0.000000 | 0.000000 | 0.000000 | 0.000000 |