CAZyme Information: MGYG000002267_05625

Basic Information

• Species: Eisenbergiella sp003478085
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eisenbergiella; Eisenbergiella sp003478085
• CAZyme ID: MGYG000002267_05625
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
821		95041.42	5.1769

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002267	7234430	Isolate	China	Asia

• Gene Location: Start: 25879; End: 28344 Strand: +

Full Sequence      

MKIQQLHQHWQMKMEGEDTWLPAKVPGSIYQDLIDGGRLEDPYWRDNEKKALAIMENHFI
YRCTFRKGENTYTKAFLRFDSLDTLTEIHLNGKLLGNTENMHRIYEYDVTGILKEDNLLE
IHFYPAVAYIRERQETIRAEGCIDAMDGFAHIRKAHSMFGWDWGPRIPDGGILRDVTISY
RDAAWIEEVHIRQKHEEGRVCLNLSVKTGKSGRDEMPLSYHARITAPDGGQIDAADSPGE
LAIEKPMLWWPNGLGSPNLYEVEIRLFSGEYLLDTWKKRIGLRTITICREKDAYGESFAT
EINGQQVFAMGADYIPQDNIFGRIQEERTRKLLESCVMANFNTIRVWGGGYYPEDSFLDL
CDEMGLMVWQDFMFACAVYELTDAFEENIRAEFIDNIRRIRHHACLALWCGNNEMEMFVA
GGGWGNTPKQMSDYFKMYKYIIPKLLKEYDPDTFYWPASPSSGGGFDDPNSYDRGDVHYW
DVWHGGKPITEFRKFYFRYLSEFGFQSFPAMKTVESFTLPGDRNIFSYVMEKHQRNNSAN
GKIMTYMGQEYLYPAKFEHLLYASQLLQAEAVKNGVEHLRRHRGRCMGAIYWQLNDIWPG
ASWSSIDYYGRWKALHYYAKRFFAPVLLSCEEESRMTQERNINAEKPDFNKSVKFNVSNE
TFEEKQVEIIWSVRSADGREKSRESVTLSVPSFSAAWTQKWELPLLDAAREYVSYSLVDI
APLSEPKDGPLSAANVVSNGSVLFTMPKYFEFEDPQLTVSCSEEMITVQSRCYARGVEIQ
NENEDLILSDNYFDMDGGEKTVRILSGTPQGLKVRSVYDIR

Enzyme Prediction     

No EC number prediction in MGYG000002267_05625.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	710	8e-107	0.7007978723404256

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.50e-98	1	679	10	671	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	1.64e-10	754	819	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	6.00e-09	186	283	3	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	7.34e-09	76	451	85	441	beta-D-glucuronidase; Provisional
pfam17786	Mannosidase_ig	0.004	653	749	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEF82182.1	0.0	1	821	1	816
QBE97789.1	0.0	4	821	18	837
QQQ93614.1	0.0	6	821	6	823
ASU28856.1	0.0	6	821	6	823
ANU76049.1	0.0	6	821	6	823

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	1.32e-151	10	806	15	817	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.40e-151	10	806	17	819	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.44e-151	10	806	17	819	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	3.78e-151	10	806	15	817	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
6BYE_A	1.19e-149	19	806	31	834	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	4.25e-113	17	633	23	663	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	1.62e-112	17	633	23	663	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	8.65e-112	17	633	23	663	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q5B7W2	1.24e-109	6	633	9	663	Beta-mannosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mndB PE=1 SV=2
Q0CCA0	2.43e-109	4	633	7	661	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000063	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002267_05625.