CAZyme Information: MGYG000002275_01419

Basic Information

• Species: Paraprevotella sp003477995
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Paraprevotella; Paraprevotella sp003477995
• CAZyme ID: MGYG000002275_01419
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1112	MGYG000002275_12|CGC2	123486.26	4.8291

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002275	3751567	Isolate	China	Asia

• Gene Location: Start: 22097; End: 25435 Strand: +

Full Sequence      

MKKNKLYITSLLALTLPAYGWAQDRISFEGNDGQATYKSIGVYDSWDESPFRSQNGANPL
LKGNVAVIDNPDVTEEGNASAKVLMVQRSRYGSNQFGARIDLNTPFELTTTTKYVHVMIK
KDKPGRVLLIGLGKRQDRAGQSPETEQCWALSSRTIEPGIWNDAVFAIKGNGGIDIHSLV
VVPDCESTHDLKEDFLVYIDEIEVNDESTPRIKIGDYPLNIEEDAVSSKNNYYVNSVALT
SPTAGEQTINVGSATPQTIYRPLLEKSFVAKAGETVTPKINFSGSWMNGYVYLDKGQDGD
FYWQLNEDYTAPENSDLVSYYYIETVENESGFNYKGEPVSGNNRNTMQTPAFKIPEDLKP
GFYRLRFKVDWGNADPGGRTTTTNGIIANGGNITDVRLNIHKDKVNVTTQYQNGDVYAAD
GSQLAGSEVKFGEPLTIKMVPSNGFIPDGITLTHGYNLTGDSLVHGTPQYTTIRIPGYAV
KEDGTLTIPASLVDGDLHILGHFKSTTSGTETGEYEVNFEKDLIITRTDRTLNSVSLTGN
EGCSFTSNMSKTPKTVYQNRLSDVAITKQGETITPSVNYTTGGPMHGYFYIDFNNDGIFT
TEIGSDHRPTFGSELLSFSYYDGYNSDGVKKGTGETNNTITFPAVTIPEFIPDGVYRARL
KIDWNNIDPKGQYGETNKLDANGGYVVDFLINISNGEKTVKLNTLNGNIYGTSNSALPYK
LTSEKVLNVVPTPVTEDYVLSEPIYIRSGLNFDGPQYIRGNQQWFADTLQVDEVNLGQTI
SVPVYGNVEITANFKAKDGAAYKMIFNDEFDGKNGERADASKWGVSKRQNATWNRFISDS
IAVAYIEDGQLVLKAIPNEDKSTDNVDMLTGALESQNKFSFKYGKVECRAKTNGYKGNFP
AIWMMPQDQSAGWPNCGEIDIFEQIDTENKSYHTVHSNWTYNLGNKNNPTSSFSKNLSMD
RYHTYGFEWNDKLLTWYVDGVKVGSYAKSTNNDALSKGQWPFDKEFYLILNQSVGNGSWA
QPADINHTYRMDVDWIRVYQKDNTTNIESTIDHSLEINTSENKIMISTGKATDVQIVNIN
GTTLYKGTVDGNKTFHVQKGVYIVNNQKVLVP

Enzyme Prediction     

No EC number prediction in MGYG000002275_01419.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	807	1039	8.7e-56	0.991304347826087

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd08023	GH16_laminarinase_like	2.85e-63	806	1039	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
cd00413	Glyco_hydrolase_16	3.08e-29	808	1039	1	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd08024	GH16_CCF	2.94e-27	873	1015	104	275	Coelomic cytolytic factor, member of glycosyl hydrolase family 16. Subgroup of glucanases of unknown function that are related to beta-GRP (beta-1,3-glucan recognition protein), but contain active site residues. Beta-GRPs are one group of pattern recognition receptors (PRRs), also referred to as biosensor proteins, that complexes with pathogen-associated beta-1,3-glucans and then transduces signals necessary for activation of an appropriate innate immune response. Beta-GRPs are present in insects and lack all catalytic residues. This subgroup contains related proteins that still contain the active site and are widely distributed in eukaryotes. Their structures adopt a jelly roll fold with a deep active site channel harboring the catalytic residues, like those of other glycosyl hydrolase family 16 members.
cd02178	GH16_beta_agarase	8.22e-22	806	1040	25	258	Beta-agarase, member of glycosyl hydrolase family 16. Beta-agarase is a glycosyl hydrolase family 16 (GH16) member that hydrolyzes the internal beta-1,4-linkage of agarose, a hydrophilic polysaccharide found in the cell wall of Rhodophyceaea, marine red algae. Agarose is a linear chain of galactose units linked by alternating L-alpha-1,3- and D-beta-1,4-linkages that are additionally modified by a 3,6-anhydro-bridge. Agarose forms thermo-reversible gels that are widely used in the food industry or as a laboratory medium. While beta-agarases are also found in two other families derived from the sequence-based classification of glycosyl hydrolases (GH50, and GH86) the GH16 members are most abundant. This domain adopts a curved beta-sandwich conformation, with a tunnel-shaped active site cavity, referred to as a jellyroll fold.
cd02182	GH16_Strep_laminarinase_like	6.08e-20	802	1040	2	259	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12302.1	3.65e-159	9	791	11	778
QFQ13169.1	7.25e-104	514	1041	24	542
QKH89566.1	4.20e-82	799	1108	45	331
QUB68183.1	8.02e-82	799	1108	45	331
QUB63276.1	1.11e-81	799	1110	45	333

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6T2R_AAA	2.20e-37	802	1040	29	262	ChainAAA, Beta-glycosidase [Bacteroides caccae]
6JH5_A	9.29e-30	802	1041	4	233	Structureof Marine bacterial laminarinase [Aquimarina]
6JHJ_A	5.85e-29	802	1041	4	233	Structureof Marine bacterial laminarinase mutant-E135A [Aquimarina],6JIA_A Marine bacterial laminarinase mutant E135A complex with laminaritetraose [Aquimarina sp.],6M6P_A Structure of Marine bacterial laminarinase mutant E135A in complex with 1,3-beta-cellotriosyl-glucose [Aquimarina sp.]
6XOF_A	9.33e-28	802	1041	24	268	ChainA, GH16 family protein [uncultured bacterium]
6XQF_A	4.29e-27	802	1041	24	268	ChainA, GH16 family protein [uncultured bacterium],6XQG_A Chain A, GH16 family protein [uncultured bacterium],6XQH_A Chain A, GH16 family protein [uncultured bacterium],6XQL_A Chain A, GH16 family protein [uncultured bacterium],6XQM_A Chain A, GH16 family protein [uncultured bacterium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9ZG90	1.16e-39	802	1040	56	288	Keratan-sulfate endo-1,4-beta-galactosidase OS=Sphingobacterium multivorum OX=28454 PE=1 SV=1
P23903	2.16e-24	556	1041	150	681	Glucan endo-1,3-beta-glucosidase A1 OS=Niallia circulans OX=1397 GN=glcA PE=1 SV=1
P45798	7.61e-22	802	1040	40	283	Beta-glucanase OS=Rhodothermus marinus OX=29549 GN=bglA PE=1 SV=1
Q27082	1.69e-15	802	1102	25	317	Clotting factor G alpha subunit OS=Tachypleus tridentatus OX=6853 PE=1 SV=1
Q8N0N3	3.02e-14	862	1025	124	311	Beta-1,3-glucan-binding protein OS=Penaeus monodon OX=6687 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000312	0.998880	0.000234	0.000202	0.000193	0.000165

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002275_01419.