CAZyme Information: MGYG000002276_01488

Basic Information

• Species: OF09-33XD sp003481995
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; OF09-33XD; OF09-33XD sp003481995
• CAZyme ID: MGYG000002276_01488
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
642		73982.69	6.7413

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002276	4093426	Isolate	China	Asia

• Gene Location: Start: 102578; End: 104506 Strand: +

Full Sequence      

MAKGGANVSKEEPARFGELDQYLFGQSTHYEIYRKMGAHPGLEKGKKGVWFTVWAPNASS
VSVVGTFNGWRPDSHPMKKVAEMGVYELFVPEALEGELYKYCIHTKDGRELYKADPYAFC
SEKRPGNASRIASIGGYKWGDALWMQKRQEFSPQTSPLSIYEVHPGSWKKHPWTESNPEG
FYNYKQMAQSLTEYVKDMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPLEFKYL
VDYLHKNKIGVILDWVPAHFPKDAHGLAEFDGEAVYEHADPRQGEHPDWGTKIFNYGRPE
VKNFLLANALYWVEEFHVDGLRVDAVASMLYLDYGREPGQWIPNKNGGNENLEAIEFFKH
LNSLVTGRNKGVMMIAEESTAWPKVTGDVHDGGLGFTLKWNMGWMHDFLEYMKLDPYFRK
YNHHKMTFSLTYAFSENYILVLSHDEVVHLKCSMINKMPGEDEDKFENLKAGYSFMIGHP
GKKLLFMGQDIGQYREWSEERELDWFLLQNKPNEHLQAYVRDLLAMYKKYPALSGNDTGS
ECFSWINANDGDRSIFSFIRWSPTGKNHLLFVCNFTPVDRPDYRVGVPRRKQYRLILDSR
NLKYGGSHEVPEVYRALKRECDGQPFSIGYDLPGYGVAVFRF

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	193	493	1.1e-155	0.9966777408637874
CBM48	35	119	5.1e-20	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	16	642	7	627	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	36	642	27	621	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	4	640	587	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	17	641	7	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	4	641	84	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM07925.1	0.0	1	642	1	642
QOV19341.1	0.0	15	642	3	630
AWY99231.1	0.0	15	642	6	634
QIB55783.1	0.0	8	642	1	636
QMW76350.1	0.0	8	642	1	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	4.30e-229	20	641	133	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	6.09e-229	20	641	133	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	8.62e-229	20	641	133	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.22e-228	20	641	133	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A	4.91e-228	20	641	133	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	8.25e-293	18	642	9	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q2RR72	3.21e-241	1	641	91	731	1,4-alpha-glucan branching enzyme GlgB OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=glgB PE=3 SV=1
A1U0K1	6.98e-236	11	641	2	628	1,4-alpha-glucan branching enzyme GlgB OS=Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8) OX=351348 GN=glgB PE=3 SV=1
Q7NL20	1.43e-235	18	641	105	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
B3PGN4	1.63e-235	15	608	114	701	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002276_01488.