CAZyme Information: MGYG000002277_00909

Basic Information

• Species: Marvinbryantia sp900066075
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Marvinbryantia; Marvinbryantia sp900066075
• CAZyme ID: MGYG000002277_00909
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
645	MGYG000002277_4|CGC1	72926.66	4.9313

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002277	3435610	Isolate	China	Asia

• Gene Location: Start: 93145; End: 95082 Strand: +

Full Sequence      

MRQEREKIRWKDIRITDSLFGSYVKRVSEKVIPHQWKILNDQLEDSEKTYCIQNFRVAAG
ELTGERKGVVFQDTDLYKWLESVAYTIAAGQGLEYEPLADEVIDLIGRAQEPDGYLNTYF
TVNAPHRKWKNLMEGHELYTAGHMIEAAVAYYEATGKTKFLDIARKNADLIGQVFGRGEG
QKRGYPGHQEIELALVKLYRVTGERRYLETARYFIEERGSCPSYFEQEVAERGGFEFFPD
FQDYDLAYSQADIPPVQQTKAEGHSVRDMYMCAAMADLAGEYEDEAMLQACRRIWDNMTQ
KQMYITGGIGSSGFRERFTTDYDLPNATNYSETCASIGLMMFGQRMSAVTGEAFYYDSVE
LALYNTVLAGINIEGDRYFYVNPLEVVPEFCTAHTYMNHVKPIRQKWFSIACCPPNVART
LASLGQYIYARDGEELVIHQFISSEVQTEAGGHNVELRMDSTLMQDGKVKISIRSEADTK
LKIRIPGYAVNWTIRAGEADLDPVVEKGYLKVTAKAGEQTLVLDLDIHPHWVAANDLVRA
DAGKAALMKGPFVYCLEEKDNGNLLSQVCVEANQKIQEAAPAEGLVGEIPTLSYPGVRVK
NVGLGDNLYGALQIEKEKTQLTAIPYCLWNNRGEGEMLVWHRVLI

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	14	555	2.3e-199	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	2.15e-175	4	640	5	584	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	3.89e-152	12	555	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG1331	YyaL	5.48e-05	142	294	414	563	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].
COG2942	YihS	1.07e-04	143	327	178	375	Mannose or cellobiose epimerase, N-acyl-D-glucosamine 2-epimerase family [Carbohydrate transport and metabolism].
COG1331	YyaL	1.24e-04	125	212	453	561	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL35447.1	1.25e-267	1	645	3	648
QMW80468.1	1.51e-244	12	645	13	650
QIB56758.1	1.51e-244	12	645	13	650
QBE96220.1	6.11e-244	12	645	13	650
AWY98667.1	3.73e-232	11	645	12	656

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	5.81e-207	12	640	15	644	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.26e-201	12	640	15	644	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	1.11e-138	12	642	2	657	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.53e-138	12	642	2	657	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	4.31e-138	12	642	2	657	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	6.08e-138	12	642	2	657	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002277_00909.