CAZyme Information: MGYG000002278_00255

Basic Information

• Species: Eubacterium_I ramulus_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_I; Eubacterium_I ramulus_A
• CAZyme ID: MGYG000002278_00255
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1972	MGYG000002278_1|CGC3	218246.94	4.7134

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002278	3656069	Isolate	China	Asia

• Gene Location: Start: 281329; End: 287247 Strand: +

Full Sequence      

MRKKLVVKMLCLAMAASMVGTMVPTSLYPITANATEVEQNADDVQAQAELTDGIDDDWTL
ENKVGDDAVCEVEGSWLHLKSGVSNGNDPGSKPAMFVNPTTFDFSKDGYFDFTIKTDATE
ATNNRFGVYLGYNTDSVGMMIGFDAGGWFWQKYGASGSPWYTGGRIASPTSGEEVNVHIE
WTAAKKVTVKIGDTVAFQNEDFSEIGGLGNKIAFKCGSYGGNATDVFVKNIHYTGQKTVD
QIKTFAVSGKVVDAEGKPIANATVAVGKQSIKTNEDGVYSINVKPGQYQLSVIRDGYESA
TQDITVGEQNLEVSDITLVKEAEMETETLSTEDMDVVVSKTFPSVARYEMKKGDLAGKVF
YGQTDKINTVKINGTAVNLSDEDVKATFEADKATYVMTVKGDKIDAVITAELVAKDNTLA
FNITDIKNNLKDSVDGNPVQTIEIPNQSLISVRSTQSGANFKGAAMSSDTKISGDQYVEV
NDKASLNRDYAYGFVSNDEMSAGLWSNSEHDGYTAATRVTGGSKNTRVQATTQKKQEYVS
LGLSSCAWYYHRVVTDSHNRSYMVKETEMPQTKIIIAGDMNEDDKIDWQDGAVAFRSIMN
NPYKSEEVPELVAYRIAMNFGGQAQNPFLTTLDNVKKVALNTDGLGQSVLLKGYANEGHD
SAHPDYADIGKRIGGADDMNTLMTEGAKYGARFGIHVNAGEMYPEAKAFKDDNVRRDESG
NLRYGWNWLDQAVGLDSIYDLATGDREARFDDLEELVGTNLDFVYVDIWGNNTGSADDDS
WQTRKLSKEINSNGWRMANEWGVANEYDATFQHWAADLTYGGSNQKGQNSEVMRFLRNHQ
KDSWVADYPKYGGAAMMPLLGGYNMKDFEGWQGRNDYDTYITNLFTHDVTTKFIQHYKVI
KWVDGEPVNAGGAANWTPDMEITLKDDDGNKLVLTRGSDNPSDAAYRNRTMTLNGKVVAQ
GAPSAGDRSDSDIQNERNRGTESYLLPWVWDSETGEKVSSSEEKLYHWNTRGGTTTWDVP
DSWAGLKNVKVYRLTDEGKTDEQTVDIVNGQLTLTAEAEVPYVVCKGEKGNIAVTWSQGM
HIVDAGFNGGAASLATNWTKVGDGTAEIAKSQYSNPMLKLSGDVAMTQELTDLKPGQQYA
VLVGVDNRSDCKASMTVKCGDEVLATNYTERSIAKNFVKAYTHSNSSATVDGSSYFQNMY
VYFTAPESGKVTLTLAKAAGEGDAYFDDVRIVENDAQNITRDKDGNIVKFEQDFENNVQG
IYPFVVSGIEGVEDNRIHLSELHAPYTQAGWDVKKMDDVLDGNWSVKINGLTQRQTLAYQ
TIPQNFRFEPGVKYRVSFDYQAGSDGTYSVVVGSGEFSEATEVDTLNKHMGKDKDGHYIF
EITGDLSGQTWFGIYSTDKAPDLQGSEGNAQNFGGYKELVLDNLVIERVDERITAKTLKK
LITKAADLDEEKYTRADWKTLSEAIDDANVALNRDKTSSEEIEACYRHLNAVLEYLSTRG
LPAMGDEEFDIPVAGVKVTAGSKDNGTHYGSKFGNPAYAIDGDTNTHWMTNYDGYNSAIK
NRECWIDFEFPEAHTVNGLRYLPGPKMGGHLTTITKFELSVKTAGSDKYQLVQEGEWENS
SGWKITEFEPVENVTNVRLLATEAKDTNWWAVASEIRVMNKVENGGDVSTGLETQIATAN
KLLSKEKQYSAKSWKTFAAKLKAAEDVMANADATQYDVKLALANLKDAMSGLQMKDGLAD
VKNEDGNWYYYKNGKIATDVTTVAKNINGWFYVKDGKVDFKANTVAKNENGWWKITDGRV
DFKANTVAKNENGWWKITNGKVDFGYTGIAKNENGWWRIVNGKVDFNCNSVEKNENGWWY
IRGGKVDFGYTGVAKNKNGWWRIVNGKVDFNCNSIEKNENGWWYIRNGKVDFSYTGVAKN
QNGWWRVENGKVNFNYNGIAKNENGWWYIRNGKVDFSYNGGTHNVVNGKVQK

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	332	1066	0	0.9957567185289957

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	2.07e-162	589	881	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	2.82e-130	602	904	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974	GalBD_like	4.44e-115	1227	1415	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080	Gal_mutarotas_3	2.27e-81	329	588	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	2.66e-38	887	1040	1	111	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK23316.1	0.0	2	1732	3	1733
BBK63058.1	0.0	2	1732	3	1733
QNM11059.1	0.0	21	1735	17	1728
QOL33684.1	0.0	58	1733	81	1765
QUI44700.1	0.0	46	1733	62	1766

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QFK_A	0.0	327	1501	10	1192	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEP_A	0.0	326	1501	9	1192	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	0.0	327	1501	10	1192	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
5A59_A	0.0	319	1429	9	1103	Thestructure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen [Streptococcus pneumoniae TIGR4],5A5A_A The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with PNP-T-antigen [Streptococcus pneumoniae TIGR4]
6QFO_A	0.0	326	1496	9	1187	EngBFDARPin Fusion 9b 3G124 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	0.0	87	1688	156	1655	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	0.0	87	1688	156	1655	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	1.92e-133	328	1427	51	1035	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001356	0.997501	0.000223	0.000436	0.000238	0.000210

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002278_00255.