CAZyme Information: MGYG000002285_00112

Basic Information

• Species: CAG-95 sp900066375
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-95; CAG-95 sp900066375
• CAZyme ID: MGYG000002285_00112
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
806	MGYG000002285_1|CGC2	92399.69	5.8799

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002285	3374269	Isolate	China	Asia

• Gene Location: Start: 130668; End: 133088 Strand: +

Full Sequence      

MDNKLYKLMNWPRVEGIIYSEENSPFEILGPHPAGRNTLVQTFWPDAKEVFLVSGTKKKQ
MEMADEAGFFACLVSGRDMADYRYEVHYDDERGIKSVKDAYRYHHGLTKQQIDKLNAGIL
YDSYEFLGAHPMEIDGCHGTFFAVYAPNAIRVSVVGDFNYWDGRIHQMQLCRESGVYELF
IPDVKAGDCYKYEIKSKGNTVVLKADPYAFGQQLRPDTASIVRNISYEWSDEAWMKERKK
LQQKDAPVSVYELYLGSFKRGEDGEYLNYRALAPLVASYAKDMGYTHVELMPVMEHPLDA
SWGYQVIGYYAPTARYGTAADFKYFVDMLHKEGIGVILDWVPAHFPRDTYGLGRFDGTCI
YEHEDPRKGAHPHWGTLLYNYARPQVSNYLISNALYWVKEYHADGIRMDAVASMLYLDYG
KKEGEWIPNIYGGNENLDAVEFLKHLNSIMKKKNPDVYLIAEESTAWPKVTGEVEEDGLG
FSMKWNMGFMNDYLSYISYDPYFRAHHHNELTFSMVYQYSENFMSVFSHDEVVHGKSTMI
GKMPGNKEDKFANLRLTYAYMMTHPGKKLLFMGQDIAEYKEFDETRQTEWGLLQYPEHKG
ISKLVKDLNFLYKNKPALYKKDTDPDGFEWINCISSDKCMLSFVRKADKLQDMILVVANF
AGIEQEFTVGVPAAGKYKELFTTDDKIYGGNSSRVTRAQATTEEEADGRENSIHVVVPSL
SLVMYSYAPYTQKELAEIERKKEAAMARRKAKEAMKEAEEARKEADLAREEAGLAQKKAE
AAEERAKAAMERAKAADKEAQEKEKA

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	279	578	1.5e-151	0.9933554817275747
CBM48	127	211	2e-17	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	107	729	7	629	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	107	732	7	626	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	14	720	508	1215	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	118	727	17	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	25	729	17	725	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM03882.1	0.0	1	742	1	757
AWY97839.1	0.0	1	744	1	746
QNM09059.1	0.0	1	748	1	754
CBK76766.1	5.51e-318	1	733	1	748
CBL36572.1	1.10e-317	1	733	1	748

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	8.57e-236	13	729	29	775	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	8.57e-236	13	729	29	775	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	1.21e-235	13	729	29	775	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.72e-235	13	729	29	775	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	2.43e-235	13	729	29	775	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q1AZ86	1.50e-244	14	729	7	721	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
B3PGN4	2.51e-244	17	728	17	737	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1
Q3JCN0	2.67e-244	14	721	19	726	1,4-alpha-glucan branching enzyme GlgB OS=Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / JCM 30415 / NCIMB 11848 / C-107) OX=323261 GN=glgB PE=3 SV=1
Q8DLB8	2.18e-242	13	724	7	747	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q608L5	8.28e-241	12	729	12	728	1,4-alpha-glucan branching enzyme GlgB OS=Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) OX=243233 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002285_00112.