CAZyme Information: MGYG000002295_01141

Basic Information

• Species: Ruminococcus_C callidus
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus_C; Ruminococcus_C callidus
• CAZyme ID: MGYG000002295_01141
• CAZy Family: GH26
• CAZyme Description: Mannan endo-1,4-beta-mannosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
540	MGYG000002295_4|CGC5	58970.94	4.0296

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002295	2957494	Isolate	China	Asia

• Gene Location: Start: 201157; End: 202779 Strand: +

Full Sequence      

MLPVVFLRTIPLGDGAILRKEQVFALAAAGVLLTGCAKIEQEESQSSASVATATTQLATE
AETVPAQQYPSVALQVPEITRQDVSMKLEAEDAAVPSGCSVAMMPRLGYSGNGYLSGLDA
KNDSSLVLNAEIPATQHYDITVVVGASAASTCKIQVDGETVYTMKTDATNNFMRVKIQGI
FLNAGACELSVAPVDGIVDIDCIELVNNTSLYDGTSEISATPVNGKATARTQQLYQFLQQ
NYGSKIITGQYASDNSGKELDEIYNITGKYPLIRFADMNAYSPNGGDSQNATAVADSLAW
AKDGGVVGLSWLWNAPMGTGTIYEKDTSYDLKTAVTDEDIAGKSLEELSAMVTEGKISQN
CYALVSDIDSISEALQPLADADVPVLWRPLPEAGGGWYWWGSDGAENYQWLWKLLYTRMT
EYHHLNNLLWVWNGQSSSFLVDSSMYDIAALDLYVEKDQTYGSRYEQYVALRNMSAGKIL
AISECSNVPDMNAMFRDNAVWSYFGLWYAPYLGEYTDNNTLMEFYNSEAALTREDFSYSE

Enzyme Prediction     

No EC number prediction in MGYG000002295_01141.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH26	223	534	1.1e-77	0.9933993399339934

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02156	Glyco_hydro_26	5.24e-40	223	507	1	282	Glycosyl hydrolase family 26.
cd04086	CBM35_mannanase-like	5.77e-10	87	205	2	118	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.
COG4124	ManB2	9.48e-08	270	454	76	256	Beta-mannanase [Carbohydrate transport and metabolism].
cd04083	CBM35_Lmo2446-like	0.001	89	205	4	125	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
pfam16990	CBM_35	0.002	107	206	19	118	Carbohydrate binding module (family 35). This is a mannan-specific carbohydrate binding domain, previously known as the X4 module. Unlike other carbohydrate binding modules, binding to substrate causes a conformational change.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL18231.1	1.45e-147	74	536	58	527
BCN30045.1	7.51e-100	85	535	49	506
CCO05604.1	1.87e-90	18	538	1	534
BCJ93286.1	2.23e-89	87	535	52	507
ABX41451.1	6.42e-87	85	532	49	503

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3ZM8_A	4.65e-54	82	535	9	446	ChainA, Gh26 Endo-beta-1,4-mannanase [Podospora anserina S mat+]
6HPF_A	2.07e-47	219	535	3	309	Structureof Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose [Yunnania penicillata]
3WDQ_A	8.25e-32	218	511	30	322	Crystalstructure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus [Symbiotic protist of Reticulitermes speratus],3WDR_A Crystal structure of beta-mannanase from a symbiotic protist of the termite Reticulitermes speratus complexed with gluco-manno-oligosaccharide [Symbiotic protist of Reticulitermes speratus]
6HF2_A	4.45e-30	227	508	50	315	Thestructure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus [Bacteroides ovatus ATCC 8483],6HF4_A The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus, complexed with G1M4 [Bacteroides ovatus ATCC 8483]
6Q75_A	8.26e-30	222	539	23	330	Thestructure of GH26A from Muricauda sp. MAR_2010_75 [Muricauda sp. MAR_2010_75],6Q75_B The structure of GH26A from Muricauda sp. MAR_2010_75 [Muricauda sp. MAR_2010_75]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49425	1.29e-52	86	535	17	456	Mannan endo-1,4-beta-mannosidase OS=Rhodothermus marinus (strain ATCC 43812 / DSM 4252 / R-10) OX=518766 GN=manA PE=1 SV=3
G2Q4H7	4.10e-52	89	535	45	475	Mannan endo-1,4-beta-mannosidase OS=Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) OX=573729 GN=Man26A PE=1 SV=1
P55297	2.86e-44	89	535	25	460	Mannan endo-1,4-beta-mannosidase B OS=Piromyces sp. OX=45796 GN=MANB PE=2 SV=1
P55298	3.53e-43	108	535	41	458	Mannan endo-1,4-beta-mannosidase C OS=Piromyces sp. OX=45796 GN=MANC PE=2 SV=1
P55296	1.31e-41	89	535	25	459	Mannan endo-1,4-beta-mannosidase A OS=Piromyces sp. OX=45796 GN=MANA PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003123	0.076138	0.920075	0.000267	0.000399	0.000013

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002295_01141.