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CAZyme Information: MGYG000002313_00929

You are here: Home > Sequence: MGYG000002313_00929

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Lactobacillus mulieris
Lineage Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus mulieris
CAZyme ID MGYG000002313_00929
CAZy Family GH68
CAZyme Description hypothetical protein
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
770 MGYG000002313_38|CGC1 83623.43 4.5199
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000002313 1675234 Isolate Japan Asia
Gene Location Start: 68526;  End: 70838  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 2.4.1.10 2.4.1.9

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH68 199 641 1.6e-143 0.988009592326139

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
pfam02435 Glyco_hydro_68 8.42e-121 200 633 4 411
Levansucrase/Invertase. This Pfam family consists of the glycosyl hydrolase 68 family, including several bacterial levansucrase enzymes, and invertase from zymomonas.
cd08997 GH68 1.58e-107 252 631 1 354
Glycosyl hydrolase family 68, includes levansucrase, beta-fructofuranosidase and inulosucrase. Glycosyl hydrolase family 68 (GH68) consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Levansucrase, also known as beta-D-fructofuranosyl transferase, catalyzes the transfer of the sucrose fructosyl moiety to a growing levan chain. Similarly, inulosucrase catalyzes long inulin-type of fructans, and beta-fructofuranosidases create fructooligosaccharides (FOS). However, in the absence of high fructan/sucrose ratio, some GH68 enzymes can also use fructan as donor substrate. GH68 retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. Biotechnological applications of these enzymes include use of inulin in inexpensive production of rich fructose syrups as well as use of FOS as health-promoting pre-biotics.
cd08979 GH_J 1.90e-23 253 621 1 291
Glycosyl hydrolase families 32 and 68, which form the clan GH-J. This glycosyl hydrolase family clan J (according to carbohydrate-active enzymes database (CAZY)) includes family 32 (GH32) and 68 (GH68). GH32 enzymes include invertase (EC 3.2.1.26) and other other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). The GH68 family consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10, also known as beta-D-fructofuranosyl transferase), beta-fructofuranosidase (EC 3.2.1.26) and inulosucrase (EC 2.4.1.9). GH32 and GH68 family enzymes are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) and catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
TIGR01167 LPXTG_anchor 9.07e-05 738 770 2 34
LPXTG-motif cell wall anchor domain. This model describes the LPXTG motif-containing region found at the C-terminus of many surface proteins of Streptococcus and Streptomyces species. Cleavage between the Thr and Gly by sortase or a related enzyme leads to covalent anchoring at the new C-terminal Thr to the cell wall. Hits that do not lie at the C-terminus or are not found in Gram-positive bacteria are probably false-positive. A common feature of this proteins containing this domain appears to be a high proportion of charged and zwitterionic residues immediatedly upstream of the LPXTG motif. This model differs from other descriptions of the LPXTG region by including a portion of that upstream charged region. [Cell envelope, Other]
pfam00746 Gram_pos_anchor 1.23e-04 730 770 1 41
LPXTG cell wall anchor motif.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QGR96269.1 0.0 1 770 13 782
QLQ61478.1 1.05e-275 142 676 143 677
AGR65155.1 2.02e-274 160 676 179 695
AAN05575.1 4.65e-273 160 676 179 695
AXX73387.1 4.65e-273 160 676 179 695

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFR_A 9.69e-259 160 675 36 557
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 [Lactobacillus johnsonii],2YFT_A Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose [Lactobacillus johnsonii]
2YFS_A 5.55e-258 160 675 36 557
Crystalstructure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose [Lactobacillus johnsonii]
3OM6_A 5.49e-89 195 639 11 449
ChainA, Levansucrase [Priestia megaterium],3OM6_B Chain B, Levansucrase [Priestia megaterium],3OM6_C Chain C, Levansucrase [Priestia megaterium],3OM6_D Chain D, Levansucrase [Priestia megaterium]
3OM7_A 7.68e-89 195 639 11 449
ChainA, Levansucrase [Priestia megaterium],3OM7_B Chain B, Levansucrase [Priestia megaterium],3OM7_C Chain C, Levansucrase [Priestia megaterium],3OM7_D Chain D, Levansucrase [Priestia megaterium]
3OM4_A 1.08e-88 195 639 11 449
ChainA, Levansucrase [Priestia megaterium],3OM4_B Chain B, Levansucrase [Priestia megaterium],3OM4_C Chain C, Levansucrase [Priestia megaterium],3OM4_D Chain D, Levansucrase [Priestia megaterium]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
D3WYV9 4.60e-257 160 675 173 694
Inulosucrase OS=Lactobacillus gasseri OX=1596 GN=inuGB PE=1 SV=1
Q74K42 1.45e-254 160 675 179 700
Inulosucrase OS=Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533) OX=257314 GN=inuJ PE=1 SV=1
P11701 1.71e-231 133 675 127 670
Levansucrase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=ftf PE=3 SV=2
D3WYW0 8.07e-209 160 674 158 680
Levansucrase OS=Lactobacillus gasseri OX=1596 GN=levG PE=1 SV=1
Q70XJ9 3.41e-205 167 678 226 744
Levansucrase OS=Fructilactobacillus sanfranciscensis OX=1625 GN=levS PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as SP

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.000268 0.999058 0.000183 0.000185 0.000160 0.000137

TMHMM  Annotations      download full data without filtering help

start end
743 765