CAZyme Information: MGYG000002355_00313

Basic Information

• Species: Clostridium_F botulinum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium_F; Clostridium_F botulinum
• CAZyme ID: MGYG000002355_00313
• CAZy Family: GT2
• CAZyme Description: Tyrocidine synthase 2

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1494	MGYG000002355_7|CGC8	174690.75	5.5278

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002355	3808262	Isolate	Italy	Europe

• Gene Location: Start: 288449; End: 292933 Strand: +

Full Sequence      

MKNNEKHYYNLTHPQKRIWYIDKVNLDSPLHNIGGCLNIYGNIYIDKMEDTINIIIKNNE
GLRLRFKEKDGQPVQYIQEFKKEKIEFLDFSKCINPREEYEKWSENLFKTNSFIENNQLY
YFAIYKISRKEYGVLLNIHHIISDGWSINLIEKQICKIYSKLINNEDISLNEYYSYVDFV
HEEQKYLKSDRFIKNKNFWREKFKDISEEFLYKTSSSLEGKRQCFNIDINLSNKIKKFID
NKKYSLNTFFIVILLIYINKTTYKKDLIIGTPVFNRTGKNQKSTVGMFTSTVPFRFTLDT
ELTVENLMKLINRELKFCFLNQKYPYDLLVKDLELSKLGYDSLFKMCVNYYNSNYVNDIN
GNKAEVKEYYCGNQSYSLQLTVKEWEENNITLNFDFKTLEYTDQEIKTMYKSMINIINQV
IENEQLKVNQIKLLNKEERNSKVYDLNSTTNYYPQKAVCELFEEQVMKTPNKVVVEFKEK
TLTYKELNEKANQLANHLREKGRDKKSIVSIMETHSIELIISILGVLKTGAAYLPIDPNY
PVERINYMLEDSKSNMLLTNFKVTDEIKFKGNIININNEDLQGYSKINLPEANSLSDLVY
IIYTSGSTGKPKGVMIEHQGLTNYIWWANKMYLKDEDEVMALYSSISFDLTVTSIFTPLI
SGNKIIIYDNDETEFVLYKILRENKATVVKLTPAHLTLLKDMDNTKSSIKRFIVGGEDLK
VKLAKEVCNSFGKNIKIYNEYGPTETVVGCMIYKYDEDKDKGVSVPIGRPADNVQIYVLD
NELNPVSTGFVGELYISGDGVARGYLNREGLSHERFIENSFIKGKRMYKTGDTARYLENG
SIEYVGRIDNQVKIRGHRIELGEIERYLIENECVKDAVVVYKEDSSKNKFLNAYLVAKKE
ITDLQLKEWLLKFLPKYMLPTNFVFMGKLPLTLNGKVNYALLPEPETVEREFIKYATLAE
KELVKAMEEILGIENISMNDNYYQLGGDSIKAIQISSKLKNIGLNIKVKDILAYDSIEEI
SAAIEESEIIRLISQDKSEGTVEKTPIVEWFFNQKFSNESFYNQYVLLEYKGKLDINKVI
IVVNKLIEHHDALRINYNKRSNELYYNDKKYNEIHAFKYFNLSEYSYKDQCKNIKKLINE
TNSSINIENSSLFNVVMMDLEDNRQALLFTAHHLIIDGVSWRVILDDFITILKQLDEGSK
IRLPLKTHSYKEWSEALQTYRRNDFSEEVGYWKSILDKEISYNVDYNNEEDIVETSNILS
KEIDEETFSSLIQKVDEIYNMDLNEALIIGLVLALNKLTKKDEIIVEFERHGREAINDYI
DVSRTVGWFTIMYPAYFSIEHEDIEDNIKSLKEQFRNIPNNGFNYSILKFLNKKLKGQES
KYVRFNYLGDFDNIIDKEKLNISDIEFGLCSDDKNSLTALIDITAIIINKKLKVTAEYSK
RRFKDEIVEKFIESYIETLKLILDKCSNKGFKEFTPSDFDAVDISQEDLDALFD

Enzyme Prediction     

No EC number prediction in MGYG000002355_00313.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd05930	A_NRPS	0.0	470	942	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK05691	PRK05691	0.0	11	1492	1731	3247	peptide synthase; Validated
PRK12316	PRK12316	0.0	11	1492	52	1546	peptide synthase; Provisional
PRK12316	PRK12316	0.0	11	1492	2605	4092	peptide synthase; Provisional
PRK12467	PRK12467	0.0	11	1239	52	1315	peptide synthase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAZ00088.1	1.69e-125	11	1027	2247	3295
BAZ75991.1	1.69e-125	11	1027	2247	3295
BAY30132.1	9.76e-125	11	1027	2249	3297
BAY90071.1	3.23e-123	11	1027	2238	3286
AFZ04852.1	2.09e-108	217	1000	348	1158

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MFZ_A	1.99e-128	4	1030	781	1803	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFX_A	9.34e-128	460	1240	209	972	Crystalstructure of a 4-domain construct of a mutant of LgrA in the substrate donation state [Brevibacillus parabrevis]
6MFW_A	2.43e-127	460	1240	209	972	Crystalstructure of a 4-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis]
2VSQ_A	1.20e-125	37	1022	41	1037	Structureof surfactin A synthetase C (SrfA-C), a nonribosomal peptide synthetase termination module [Bacillus subtilis]
6MFY_A	3.08e-124	460	1240	209	972	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9R9J0	1.93e-259	9	1492	1319	2816	Mycosubtilin synthase subunit B OS=Bacillus subtilis OX=1423 GN=mycB PE=3 SV=1
O68007	1.11e-243	2	1492	1100	2598	Bacitracin synthase 2 OS=Bacillus licheniformis OX=1402 GN=bacB PE=3 SV=1
O68008	1.36e-219	2	1492	1045	2533	Bacitracin synthase 3 OS=Bacillus licheniformis OX=1402 GN=bacC PE=3 SV=1
O68006	2.35e-219	2	1494	2699	4199	Bacitracin synthase 1 OS=Bacillus licheniformis OX=1402 GN=bacA PE=3 SV=1
P39847	3.66e-208	9	1492	1058	2548	Plipastatin synthase subunit C OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsC PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000058	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002355_00313.