CAZyme Information: MGYG000002359_02508

Basic Information

• Species: Mycobacterium tuberculosis
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Mycobacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis
• CAZyme ID: MGYG000002359_02508
• CAZy Family: GH23
• CAZyme Description: Resuscitation-promoting factor RpfD

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
154	MGYG000002359_7|CGC1	15678.51	4.2396

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002359	4387829	Isolate	Sweden	Europe

• Gene Location: Start: 48478; End: 48942 Strand: -

Full Sequence      

MTPGLLTTAGAGRPRDRCARIVCTVFIETAVVATMFVALLGLSTISSKADDIDWDAIAQC
ESGGNWAANTGNGLYGGLQISQATWDSNGGVGSPAAASPQQQIEVADNIMKTQGPGAWPK
CSSCSQGDAPLGSLTHILTFLAAETGGCSGSRDD

Enzyme Prediction     

No EC number prediction in MGYG000002359_02508.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam06737	Transglycosylas	9.79e-37	50	121	1	75	Transglycosylase-like domain. This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.
cd13925	RPF	1.46e-27	53	121	2	71	core lysozyme-like domain of resuscitation-promoting factor proteins. Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.
cd00442	Lyz-like	1.00e-05	54	106	2	56	lysozyme-like domains. This family contains several members, including soluble lytic transglycosylases (SLT), goose egg-white lysozymes (GEWL), hen egg-white lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, chitosanases, and pesticin. Typical members are involved in the hydrolysis of beta-1,4- linked polysaccharides.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVK90546.1	1.30e-110	1	154	1	154
EGE50938.1	1.30e-110	1	154	1	154
CCK56434.1	1.30e-110	1	154	1	154
AGE68386.1	1.30e-110	1	154	1	154
AIH35874.1	1.30e-110	1	154	1	154

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2N5Z_A	8.03e-31	53	125	8	80	Mycobacteriumtuberculosis: a dynamic view of the resuscitation promoting factor RpfC catalytic domain [Mycobacterium tuberculosis H37Rv]
4OW1_A	1.07e-30	53	125	5	77	ChainA, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_B Chain B, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_E Chain E, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_S Chain S, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_T Chain T, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_U Chain U, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_W Chain W, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_X Chain X, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis]
4CGE_A	1.24e-28	52	121	2	71	ChainA, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_B Chain B, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_C Chain C, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_D Chain D, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_E Chain E, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_F Chain F, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis]
4KL7_A	3.16e-22	54	123	3	75	ChainA, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_B Chain B, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_C Chain C, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_D Chain D, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_A Chain A, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_B Chain B, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_C Chain C, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_D Chain D, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis]
4EMN_A	3.25e-22	54	123	4	76	ChainA, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_B Chain B, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_C Chain C, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_D Chain D, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P9WG27	2.59e-111	1	154	1	154	Resuscitation-promoting factor RpfD OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=rpfD PE=1 SV=1
P9WG26	2.59e-111	1	154	1	154	Resuscitation-promoting factor RpfD OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=rpfD PE=3 SV=1
H8F3N4	8.87e-32	27	125	9	108	Resuscitation-promoting factor RpfC OS=Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman) OX=652616 GN=rpfC PE=2 SV=1
O07747	2.42e-31	27	125	45	144	Resuscitation-promoting factor RpfC OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=rpfC PE=1 SV=3
O53177	7.04e-27	49	121	96	168	Resuscitation-promoting factor RpfE OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=rpfE PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999803	0.000188	0.000001	0.000001	0.000000	0.000015

TMHMM  Annotations     

start	end
21	43