CAZyme Information: MGYG000002372_00877

Basic Information

• Species: Clostridium_P perfringens
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium_P; Clostridium_P perfringens
• CAZyme ID: MGYG000002372_00877
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2104	MGYG000002372_1|CGC17	236080.25	4.4638

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002372	3343822	Isolate	South Korea	Asia

• Gene Location: Start: 1031311; End: 1037625 Strand: +

Full Sequence      

MFSKKFKKQMAYVLASTFVFGLVNPSAIKVRASTGVEITEGVTVTAKGNTEGNTADLAID
GDLSTYWESSNDYKWIEVDLGGIYELSKIEIFNKDEAVYKYNIYASEDGENFNKIAYKNN
DNVSDSNGNVHTIDNVRAGKIRIDVVQNSNSDRVNIAEINVFGKNTGESLPEIKKIATSN
FSETPWATEYEKFNSDIAYANEKTLNEIKNLVGRVIGEEFKDKFIFEIRDQLNGNDVFEV
SDSRDGKVLIKGNNGVSLASGFNYYLKNYCNVSYNPIMGSNLKMPETMPSVGERVVIDTP
YEYRYALNFCTYSYTMSFWDWDQYEEFLDWCAMNGVNLVLDIIGQEEVLRRTLNEFGYSD
EEVKEFISGPAYFAWFYMQNMTGFGGPLPNDWFEQRAELGRKMHDRMQSFGINPVLQGYS
GMVPRDFKEKNPEAQTISQGGWCGFDRPDMLKTYVNEGEVDYFQNVADVFYEKQKEVFGD
VTNFYGVDPFHEGGNTGDLDNGKIYEIIQNKMIEHDNDAVWVIQNWQGNPSNNKLEGLTK
KDQAMVLDLFSEVSPDWNRLEERDLPWIWNMLHNFGGRMGMDAAPEKLATEIPKALANSE
HMVGIGITPEAINTNPLAYELLFDMAWTRDQINFRTWTEDYIERRYGKTNKEILDAWNII
LDTAYKKRNDYYQGAAESIINARPGFGIKSASTWGHSKIVYDKSEFEKAIEIFAKNYDEF
KDSDAFLYDFADILKQLLANSAQEYYEVMCNAYNNGNGEKFKFVSGKFLELIKLQERVLS
TRPEFLIGNWIEDARTMLKDADDWTKDLFEFNARALVTTWGSRNNADGGGLKDYSNRQWS
GLTEDYYYARWEKWINGLQAELDGGAKAPNIDWFKMEYDWVNKKSDTDKLYPTEASNENL
GELAKIAMESYSVTNMDKILGENESVERVNIALGKPVSSETHGETEANPLTNITDGNTGT
MWVADKEGYPASVTVDLQGVKSVDGIEFAFRNQAGDRAVAYKVEVMDESGQWKTVSDKSE
DYSSKGVIEKVDFTGKAQKVKVSLTNVDLNLSPSYKIPELAEIMVYGGKEKEDEYTNVAL
GKPVTGAEAENGKPLANITDGDLNSLWVSNNGQTPATTSIDLQETENVEFLELHFEKPGL
RFQFKVEVEDANGNKETLLDMTSNKEDNEKSYKIPVGKDINKVHVTMTGKAEGGQHPGAW
AAIAEVKAMSKEKPANYVNIAENKNVSGSNSQSGNPLSNITDGDLSSLWISDNGAMPANA
TIDLEGNSFVDFLELHFEKEGFRFKFKVEVEDESGNRETVLDMTSNTEDNKKSYNIPVKK
EISKIHATITGKADGGEYPLAWAAIAEIKAMSKEAINKFEQASIALPQGNMKATATSEHP
DVGNEGLAKFAIDGKENTIWHTKYNPVDNLPQSITLELGGSYEINKFTYLPRSGSKNGNI
TKYEFHVSEDGNDFRKVSEGNWEDSGSLKTLKFNSTKATHVKLVALEGVGGFASAAELNV
FAIPENLAMKYETKVETTSNNAEVNNLRDGNENTLWIPRQEEEKSVTFDLSKEKDISSID
IVSKGSSPLKYSIEISNDGTEWTKIVDENNNEENKAVYSNILKSGEIGRFVRFNFNSENV
KIGEIKIYKGDSTSSLASYIRVVEDIYNSAVVGDMAGNYTEEAKESLGNAINEAKLALGN
DINSKEANEVMSTLKSALNEFYKGFVQINRTKLAALISDVDILLNEILSSLMNSMNYNEK
AIVNAEIQKLKLENNEAKEVYNKKQVSQSEIDTAYDELKNTLDSYYDAVSAETAYKSMVL
LAKEKVENAVVGNGNGQYPKEAVDALNEAIRIAEEGFESSTSLEDIRNITRTLKEAIDTF
VESVNVIDKAQLEEIISSIESKLENAEGVYIPNAIEELRKALDKAKEVLNNEGANEENIN
KAISDLTEAEAKFELSKVPNKDELNSEIERAYDVLEKLKGFDHLEGIKIKLEDAILEAER
VKNDENSLKVDVDKVLGELKIVIEEALKSIDSSEVIVSPVRDFKATNISKKNVTVQWSAP
ENSFGLEGYVLYKDGKKVAELGKDETTYKFKGLNRHTIYNFKIAAKYNNGELSSKESITL
RTER

Enzyme Prediction     

EC	3.2.1.50

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	245	905	7.1e-241	0.9909502262443439
CBM32	1374	1498	2.5e-21	0.9435483870967742
CBM32	45	159	3e-19	0.9193548387096774
CBM32	1516	1624	4.3e-18	0.8870967741935484
CBM32	938	1060	2e-16	0.9354838709677419

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	2.08e-156	304	629	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	5.54e-92	637	905	1	258	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	1.75e-28	206	289	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.
pfam00754	F5_F8_type_C	4.87e-20	1372	1498	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	2.23e-16	43	159	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOY53263.1	0.0	1	2104	1	2104
AXH51964.1	0.0	1	2104	1	2104
BAB80572.1	0.0	1	2104	1	2104
BAI70446.1	0.0	1	2104	1	2104
ALG48242.1	0.0	1	2104	1	2104

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MFK_A	0.0	32	921	6	895	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	0.0	33	921	22	910	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
2VC9_A	0.0	35	921	1	887	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
4XWH_A	1.52e-91	208	911	9	712	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]
4A41_A	5.42e-84	1365	1502	24	161	CpGH89CBM32-5,from Clostridium perfringens, in complex with galactose [Clostridium perfringens],4A44_A CpGH89CBM32-5, from Clostridium perfringens, in complex with the Tn Antigen [Clostridium perfringens],4A45_A CpGH89CBM32-5, from Clostridium perfringens, in complex with GalNAc- beta-1,3-galactose [Clostridium perfringens],4AAX_A CpGH89CBM32-5, from Clostridium perfringens, in complex with N- acetylgalactosamine [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	2.05e-97	226	900	64	794	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	7.78e-91	208	911	32	735	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2
Q0TR53	4.26e-35	2012	2104	909	1001	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	4.26e-35	2012	2104	909	1001	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
P0DTR4	2.81e-23	1366	1502	509	645	A type blood N-acetyl-alpha-D-galactosamine deacetylase OS=Flavonifractor plautii OX=292800 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000274	0.999036	0.000188	0.000170	0.000162	0.000146

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002372_00877.