CAZyme Information: MGYG000002373_00934

Basic Information

• Species: Clostridium septicum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium septicum
• CAZyme ID: MGYG000002373_00934
• CAZy Family: GH110
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1455		163034.96	4.7768

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002373	3298966	Isolate	France	Europe

• Gene Location: Start: 43174; End: 47541 Strand: -

Full Sequence      

MKTEFNKIKQKILRLLSITIVATMGATMASPTFKSNAEENTNVKTINVTDFGADPSGKND
SALAIYNALEEAKKLSEPVIINFPKGEYSIYKDYAQEREYHTSNTNSIENPMKKIGILIE
GQKDLTIEGNDSLFVIHGDMMALAVVKSENIKLRNFAWDFEVPTTTEMTVKDIGVENGKK
YVDYFIPSNFKYEIVDNNTNILWTSEKSPYTGEYYWTQKGHHNAWSVVIYNPKTEITRRH
SLSEGPFNGVRDIKKTSENTVRITYNNNIPVGQTLGVVNEMCASTHRHTAGAFIWESKNT
SIENVNVHYMDGFGWLTQMSENVTFDTVNFMPRVGTGKYTTSFADLIHVSGAKGKISIKN
SNFSHAHDDPINIHGTFTRVEEKIDNRTLKLKYIHGQQGGFPQYYVGDKVAFFTRDTLES
PSREEVLYEVVESSQPGENGNDLKTMIVKFNKDLPEDINSKIGNQPKYVAENVTYTPELE
IVNNVFHTIPTRGILATTRKPVLIEGNTFKNMSMASIFLSNDSNEWYESGPIRDMTIRNN
EFHITDTGQTEWADAPAVFIHPVTLGGKLPSSDTPIHKNITIEENEFYMNASRVVTAESV
ENLTIRNNKVFRENPNVTLNLTSPKSELKIGDNLLLDLDANGNVNSGNNREVFSFKACKN
VVIEGNSYDDGLIKNAIVRDMPLEYVNNNDSGLTLNGSGQVSPPTSNIKYVTLNPEVIIV
DNNGKVNAVGAGTGKVIAYYIWNDTEIKSNEIEFTVLSDEAVKPEEFEIVNGDNEKIEIK
DGEIQFEAIVKPESLASSIMWSVKNIDNTATDKATISENGLFTAKKEGFVIVNATLNGIS
KSRVVRISFSEDEGLSYSFNILNENNEKWESINDTELKITMERGDLYNSDNSAKNIFLYN
LPGNIDKDNMRLSVKISNLPLKTNNRWDSVGVYLFKDADNYTSIGKKSHFNGVVSVKETN
GSAVENDKNVNVQESTLIFDLYKNNNQLVINYKTPNGNWIEWGTIDATEISNDYKIGFAA
WNHDGRATDVNISEFKVANGSEYDKDSIKTLNSIKFTGVSNIEPSVREVNLNKSEAKVSE
TVSVDYLYSDLEGHNEGDSIYRWQLKNNDNTEDYFTQEKNFVPNSIGKLSCTVYPVDEHG
KVGEGVKSNEIFIVNREEIVSNADLEFININNSVIDSFDKDKVNYQYNSISNQESLKLNV
KSYEENSNIVITVDGEELIKGLGNISGNIKVNKEKDIIIKVVSPNGENEKVYSINIKKYN
NNFSKISSISILELNLNESGNLNDVYTLSTSYDTKSITLNISSEEIIGNINLYSTEYRNP
VNNNLNQHNEFQGIVDLKVGNNTFYVEGVGKDGKTKSKHIINILRTSDSKTELSDIKING
KTIEDFSSDKLKYLIKSNEKPLNIEAVPVGNSNISIKDLAIASKNYNMSIPEYDLNDDGV
VGAYEVDYISGKILE

Enzyme Prediction     

No EC number prediction in MGYG000002373_00934.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH110	46	618	2.7e-157	0.958029197080292

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12708	Pectate_lyase_3	6.60e-05	46	90	2	46	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
pfam12733	Cadherin-like	1.41e-04	1173	1256	5	86	Cadherin-like beta sandwich domain. This domain is found in several bacterial, metazoan and chlorophyte algal proteins. A profile-profile comparison recovered the cadherin domain and a comparison of the predicted structure of this domain with the crystal structure of the cadherin showed a congruent seven stranded secondary structure. The domain is widespread in bacteria and seen in the firmicutes, actinobacteria, certain proteobacteria, bacteroides and chlamydiae with an expansion in Clostridium. In contrast, it is limited in its distribution in eukaryotes suggesting that it was derived through lateral transfer from bacteria. In prokaryotes, this domain is widely fused to other domains such as FNIII (Fibronectin Type III), TIG, SLH (S-layer homology), discoidin, cell-wall-binding repeat domain and alpha-amylase-like glycohydrolases. These associations are suggestive of a carbohydrate-binding function for this cadherin-like domain. In animal proteins it is associated with an ATP-grasp domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE35188.1	0.0	1	1398	1	1398
QAS60591.1	0.0	1	1398	1	1398
ATD55583.1	0.0	46	695	3	616
ATD56740.1	0.0	46	695	3	616
QBJ75880.1	0.0	46	695	5	618

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7JW4_A	2.18e-47	42	608	24	564	Crystalstructure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta],7JW4_B Crystal structure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta]
7JWF_A	9.50e-47	42	608	24	564	Crystalstructure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_B Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_C Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_D Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B1V8K7	1.52e-127	39	759	28	726	Alpha-1,3-galactosidase A OS=Streptacidiphilus griseoplanus OX=66896 GN=glaA PE=1 SV=1
Q826C5	2.02e-113	46	612	41	585	Alpha-1,3-galactosidase A OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=glaA PE=3 SV=1
Q8A2Z5	2.24e-82	57	611	25	533	Alpha-1,3-galactosidase A OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=glaA PE=3 SV=1
Q64MU6	2.66e-81	45	612	25	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=glaA PE=3 SV=1
Q5L7M8	4.24e-80	45	612	25	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow) OX=272559 GN=glaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001798	0.996266	0.001208	0.000323	0.000215	0.000188

TMHMM  Annotations     

start	end
12	29