CAZyme Information: MGYG000002373_02351

Basic Information

• Species: Clostridium septicum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium septicum
• CAZyme ID: MGYG000002373_02351
• CAZy Family: GH84
• CAZyme Description: Hyaluronoglucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1820		205383.09	4.6074

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002373	3298966	Isolate	France	Europe

• Gene Location: Start: 31392; End: 36854 Strand: -

Full Sequence      

MNSRKKRRNSLLAFFMTFTMILGLAPNIYTQAVESAENEKINKDYEIYPIPQSIVYNGGN
LNLSSEVNVIFEDGLDQATKDRLIEVLEIKNISHTIGTEIDESKTNFLIGIQNSSGIVDN
YFAEKNLKDAEHFKHMDSHIVSVDNNVVAVLGKDTDSAFYGITTLKMIFNQLESNEIRNL
LIKDYADAQWRGFIEGYYGIPWSNENRMSLMEFGGDIKMNAYMFAPKDDPYHNSQWREPY
PEEKLAELAEMVKVGSKSKNKFIWTIHPFMHNAIRFDTEEHYQEDLAIIIRKFESLYDIG
VRQFGILGDDAGGNATDQVKLMTDLEEWKKSKGDVYNLIFVPKEYCQSFAGNNINSPYLK
TISQMPKDIEIMWTGSGVCGWVTTDTFNWFMQACSTAENPSRLPFMWLNWPVNDINKKRL
VMGKGQMLDPTVTNFKGIVTNPMQQAQASKVALFAVADYTWNRDTFNADKSWEDSFKYID
KDAGSELYTLAKHMSDPAPNGHGLVQDESEEIKSLLDEFNRKYLAGESIKEIGQTLINEY
EKIVKAADDFDTKSKNEGLKAEIDPWRQSLKALAQSTIEYIKTAIAIEDGNNGEVWSHYS
EGAAKFELSKTFQVQNIHGKDYVEAGYKRLIPFVKKLGDSLAGKVGDIVNPEEEKLNVTP
YTNVNNVYQGNIQNIVDGDLNTKVWFGRYIQEGDYVALELSKSTKITSLSIEQGKNATDK
DAFHYGKFQYSMDGETWTDVNNKTYGPYQNKVEVSNLDIEAKYVRFIVTGEIKAEGEANP
KWASFREFTINKDINEGDLSLSLIKSSNIGKRSGGNESDILRPEGVSSPIMYAKAPYEGA
DRDGIPQDAYIGVDLGREARLGKVHIVGDKSVPGDIFVNAVLEYSVNGEDYTEIETFTNK
VEINKDYSQENIIARYVRLRNKTKTNVWARFTKFNVTESNLNTKIYTNVAALANNKVNIQ
NDVAIISNLNEITLATNEYVGIKLPRIKDLTEIVKNYTESENLTLETSMNEIEWETINNL
NELQDARYIRIINKTENPIAFNLNNLEVHSLEYQGKTLKDTNFTAVENTDKAFDNDWTTQ
AWFKNNQDQGKYFTYDLGRLININSLKMVVNDYEHDYIRNGVIKASEDGNTWTDVLTIGG
NLDGSAEISDAFPNHEISYNTISVAGLNINARYLRVEVTKTLNIAKWVRFNEIIINNGQY
IPSENNPTFVSNPIEVAGYAPSNLIDGNLNTTYKPNKQDLTSGSLVYRISENTEITKINI
LQSPNTISNALVLVRTVDGWQEIGRLDRSLNDFITKGLGNILEVKLEWNEVAPTLHEIIL
SNGDQNVDKSYLESKLEEARNIDTSNWTQESKNALKDAIDEGDRVLEDKYASQNKVDDLI
RLLSTIMENPEIVDTNKEELQALYNVNKDRVEADYTVSTWAIFNEALKGAEVILEKADVT
QEEVNEAKANLENAVNQLKEKAKFVRHLEIAVEEGKKVSQEELDKVVPAVVEEFKAALAQ
AENLLNSNDAAQEDIDASFHRLSKAMHMLSFYKGDKKELIALVAKINSLNSSDYITETWE
ALQIVLEKTNKVIEDENALEEEVSTAYEELLKAFLDLRLKPSKEKLEELIKKAESLDSNK
YTEKTWNALEEELATAKNVLENEKASEKEINKITKSLELAINALEEKENNSNEVDKEKLV
QLIEKANKYLDSSEEYTNESYEAFLVSLDEANKVNLNKNATQKMVDDVIEKLNKAIEGLV
KKNEEKPGDNKPSTDNPGSNGNNSVDKNNGDNTSSGSNGIIQGSSNRKLPQTGGGVMLGY
LGIGLLALGGIVTGKRRRKN

Enzyme Prediction     

No EC number prediction in MGYG000002373_02351.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	191	491	6.3e-104	0.9932203389830508

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	1.39e-127	191	490	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam02838	Glyco_hydro_20b	2.92e-19	44	184	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
pfam00754	F5_F8_type_C	3.27e-11	669	788	11	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
COG0419	SbcC	1.76e-10	1301	1694	345	756	DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair].
pfam00754	F5_F8_type_C	1.85e-10	1061	1193	6	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QAS61771.1	0.0	1	1820	1	1820
AYE33607.1	0.0	1	1820	1	1820
AYE33902.1	0.0	1	1742	1	1758
QAS62053.1	0.0	1	1742	1	1758
AYE33868.1	0.0	1	1458	1	1087

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6PWI_A	7.21e-254	23	637	13	620	Structureof CpGH84D [Clostridium perfringens ATCC 13124],6PWI_B Structure of CpGH84D [Clostridium perfringens ATCC 13124]
6PV4_A	7.89e-173	45	650	32	653	Structureof CpGH84A [Clostridium perfringens ATCC 13124],6PV4_B Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_C Structure of CpGH84A [Clostridium perfringens ATCC 13124],6PV4_D Structure of CpGH84A [Clostridium perfringens ATCC 13124]
5MI4_A	3.49e-83	135	766	88	705	BtGH84mutant with covalent modification by MA3 [Bacteroides thetaiotaomicron VPI-5482],5MI5_A BtGH84 mutant with covalent modification by MA3 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482],5MI6_A BtGH84 mutant with covalent modification by MA3 in complex with Thiamet G [Bacteroides thetaiotaomicron VPI-5482],5MI7_A BtGH84 mutant with covalent modification by MA4 in complex with PUGNAc [Bacteroides thetaiotaomicron VPI-5482]
2J4G_A	8.94e-83	135	766	76	693	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482],2J4G_B Bacteroides thetaiotaomicron GH84 O-GlcNAcase in complex with n-butyl- thiazoline inhibitor [Bacteroides thetaiotaomicron VPI-5482]
2J47_A	9.14e-83	135	766	77	694	Bacteroidesthetaiotaomicron GH84 O-GlcNAcase in complex with a imidazole-pugnac hybrid inhibitor [Bacteroides thetaiotaomicron VPI-5482],2W4X_A BtGH84 in complex with STZ [Bacteroides thetaiotaomicron VPI-5482],2W66_A BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W66_B BtGH84 in complex with HQ602 [Bacteroides thetaiotaomicron VPI-5482],2W67_A BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2W67_B BtGH84 in complex with FMA34 [Bacteroides thetaiotaomicron VPI-5482],2WCA_A BtGH84 in complex with n-butyl pugnac [Bacteroides thetaiotaomicron VPI-5482],2XJ7_A BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XJ7_B BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine [Bacteroides thetaiotaomicron VPI-5482],2XM1_A BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM1_B BtGH84 in complex with N-acetyl gluconolactam [Bacteroides thetaiotaomicron VPI-5482],2XM2_A BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],2XM2_B BtGH84 in complex with LOGNAc [Bacteroides thetaiotaomicron VPI-5482],4UR9_A Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],4UR9_B Structure of ligand bound glycosylhydrolase [Bacteroides thetaiotaomicron],5FKY_A Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FKY_B Structure of a hydrolase bound with an inhibitor [Bacteroides thetaiotaomicron],5FL0_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL0_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_A Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron],5FL1_B Structure of a hydrolase with an inhibitor [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P26831	3.06e-314	45	1526	39	1565	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q89ZI2	7.91e-82	135	766	98	715	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
Q8XL08	1.85e-74	49	811	48	791	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q0TR53	2.48e-74	49	811	48	791	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
O60502	1.59e-27	192	467	63	337	Protein O-GlcNAcase OS=Homo sapiens OX=9606 GN=OGA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.008070	0.987403	0.003281	0.000673	0.000278	0.000238

TMHMM  Annotations     

start	end
11	33
1792	1814