CAZyme Information: MGYG000002383_01923

Basic Information

• Species: Lacticaseibacillus zeae
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus zeae
• CAZyme ID: MGYG000002383_01923
• CAZy Family: GH18
• CAZyme Description: Endo-beta-N-acetylglucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
396	MGYG000002383_13|CGC12	45212.35	6.9267

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002383	3001027	Isolate	Italy	Europe

• Gene Location: Start: 604939; End: 606129 Strand: +

Full Sequence      

MKKGFKLRWVGWLIIASGIFLSLMVKPQSVSASAETPQKTFMVYYRAWRDQAMHGVNTTI
LDPNPQSMLDLPYGIDIINVFSYVPAGQEAKAQPFFDKLKTVYAPEMHRRGAKLVRALDY
GRMVDGLIQQYGKHPTASEIDDYVQTLIYELSGQWGLDGIDIDMEQYPDAEKVALSDRII
QTMGQYLGPKAGNGTLLIYDTNGSYLVPFKNVMGYFSNLGYQQYGSGPNRTEKMRQTYTA
ASFPQDRLLAGLTFPEEGDQNRWYDTDPNHFLRSNMHTVATFSRENLGGMFVYAVDRDGR
TYEEPDFSHIRKTTYRWTKTAILETKGYSLNEIKAAAYRHLKKIAPQISPIQYQLLHRQI
NQTTNAFEVNSVFMKDDFNGAVDPTFDAVKEMQMDM

Enzyme Prediction     

No EC number prediction in MGYG000002383_01923.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06542	GH18_EndoS-like	1.16e-32	39	312	1	255	Endo-beta-N-acetylglucosaminidases are bacterial chitinases that hydrolyze the chitin core of various asparagine (N)-linked glycans and glycoproteins. The endo-beta-N-acetylglucosaminidases have a glycosyl hydrolase family 18 (GH18) catalytic domain. Some members also have an additional C-terminal glycosyl hydrolase family 20 (GH20) domain while others have an N-terminal domain of unknown function (pfam08522). Members of this family include endo-beta-N-acetylglucosaminidase S (EndoS) from Streptococcus pyogenes, EndoF1, EndoF2, EndoF3, and EndoH from Flavobacterium meningosepticum, and EndoE from Enterococcus faecalis. EndoS is a secreted endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes the glycan on human IgG between two core N-acetylglucosamine residues. EndoE is a secreted endoglycosidase, encoded by the ndoE gene in Enterococcus faecalis, that hydrolyzes the glycan on human RNase B.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARY92629.1	1.48e-289	1	394	1	394
QXG58516.1	9.88e-284	1	396	1	398
QVI36725.1	9.88e-284	1	396	1	398
QVI33518.1	9.79e-253	42	396	1	355
QVI34505.1	3.06e-250	1	396	1	396

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7PUJ_A	1.32e-109	31	391	3	361	ChainA, Beta-N-acetylhexosaminidase [Enterococcus faecalis],7PUK_A Chain A, Beta-N-acetylhexosaminidase [Enterococcus faecalis],7PUK_C Chain C, Beta-N-acetylhexosaminidase [Enterococcus faecalis]
6KPL_A	1.85e-28	44	322	35	292	CrystalStructure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris in apo form [Cordyceps militaris CM01],6KPM_A Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris in complex with L-fucose [Cordyceps militaris CM01]
6KPN_A	2.45e-27	44	322	35	292	CrystalStructure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris D154N/E156Q mutant in complex with fucosyl-N-acetylglucosamine [Cordyceps militaris CM01],6KPO_A Crystal Structure of endo-beta-N-acetylglucosaminidase from Cordyceps militaris D154N/E156Q mutant in complex with fucosyl-N-acetylglucosamine-Asn [Cordyceps militaris CM01]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B7GPC7	4.43e-93	29	388	38	415	Endo-beta-N-acetylglucosaminidase OS=Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) OX=391904 GN=Blon_2468 PE=1 SV=1
P36912	5.58e-19	44	322	66	330	Endo-beta-N-acetylglucosaminidase F2 OS=Elizabethkingia meningoseptica OX=238 GN=endOF2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001033	0.998121	0.000298	0.000187	0.000165	0.000166

TMHMM  Annotations     

start	end
7	25