CAZyme Information: MGYG000002383_02202

Basic Information

• Species: Lacticaseibacillus zeae
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus zeae
• CAZyme ID: MGYG000002383_02202
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
775	MGYG000002383_14|CGC6	84859.86	5.3356

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002383	3001027	Isolate	Italy	Europe

• Gene Location: Start: 133622; End: 135949 Strand: +

Full Sequence      

MSPVNLNSLTTEEKVKLTSGKDFWSSEAFPEHGIPSIRMSDGPHGLRYQAVEADHLGIND
SVPSTSFPTASASASSWDPELIAAMGEAIGLEARSLGVDVVLGPGVNMKRNPLCGRNFEY
FSEDPFLAGKLGAAWIQGIQKQGVAACLKHFAANNQENGRLSSNSIIDPVALHEIYLEAF
RIAVTEGHPESVMCSYNKVNGRYASDNHYLMTQVLRNQFGFDGAVITDWGALNDKVASLN
AGTDLEMPGDQHLFDKEALKAYQTGELRPASLDRAAANIAKMARKQRPKFTGSREDLLHQ
HAQLAQKIAESAIVLLKNDDEILPLAANEQVAVVGELAKETRFQGAGSSHIKAAEQVPIL
TGPKQAGIAYDYAAGYRLDDQVDEEAIAQALELARTHDKIIFVAGLPDNYESEGFDRQNM
ALPKVQNDLLQAIAAINPHVVVLLVAGAPVELPWLDQVQAVLDLYLGGEQIGAAAGRVLT
GQVNPSGKLAETYPVHYADIPSAELYDHRTRSVPYVESTYIGYRYFDKAQVPVAFPFGFG
LSYTSFAIKNARLTNDHVTDDQPVTVQVELTNTGSRDGAEVVQGYVQEQTARPLRPIREL
KAFKKVFVKAGETVTVTLALPAQAFKEWHEQTQTWELPDAAKAVAIGTSSADIVSVLPVQ
VHGEAFTNFENVPDWYTTPSGKPTVHDFEQLTGQKVPPHHEFVPGEFTRLNTPREMQKYS
WLLRLVARTVIKMRTKDYIDKDGPEAKFQQAIVLDTPLIRLAQQASGALRLSMVD

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	30	248	5.5e-72	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	7.83e-97	65	653	118	757	beta-glucosidase BglX.
COG1472	BglX	7.88e-72	33	341	59	367	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	1.12e-58	9	618	60	740	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	3.00e-54	313	543	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	2.13e-38	65	280	86	314	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAN73657.1	0.0	1	775	5	779
QPC15966.1	0.0	1	775	5	779
QVI32658.1	0.0	1	775	5	779
QXG60232.1	0.0	1	775	5	779
QVI38420.1	0.0	1	775	5	779

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	2.48e-197	6	662	9	669	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	1.09e-170	5	685	8	691	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	4.89e-123	1	653	1	705	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	7.39e-122	1	653	1	705	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	1.34e-112	6	666	10	841	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	1.36e-196	6	662	9	669	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	4.01e-169	5	685	8	700	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	3.52e-167	5	685	8	700	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
A7LXS8	1.28e-123	6	653	50	737	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1
Q5BFG8	1.66e-115	6	666	15	843	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002383_02202.