CAZyme Information: MGYG000002388_00857

Basic Information

• Species: Lacticaseibacillus paracasei
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus paracasei
• CAZyme ID: MGYG000002388_00857
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 1F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
927		100512.94	6.9537

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002388	3076437	Isolate	China	Asia

• Gene Location: Start: 870395; End: 873178 Strand: -

Full Sequence      

MKKFFDRVKTLGTRFRHWFTAFVTRRPDSEAETTNLTGKEAVVFYRNVTLQSIKTIVYYL
LGVLGIATVFGLGLFGGYFVSIIDATPIPTEAAMKATLSNTSRTSSMYFAHNVKLSNVKS
DLVSTKVDLNEMSPWLTKAIVATEDEDFYRHDGVVPKAVIRAFFSDLTGMGNQTGGSTLT
QQVVKMMFLSSETTFKRKAAEIMLARRLNNHFSKDQILATYLNVATLGRNNKGQNIAGVE
AAAQGLFGVSAKDINLPEAAFIAGLPQSPFIYTPYTASGALKSNLKDGVDRQQTVLFRMY
RAGVISHKQYVDAKAFDIKGAFLQHENAEEDDNQYGYVYNMVLSEAESLLAEQLAKNDGH
SAAELAKDNALNNDYLRQAANLFSSKNYQIKSTINKDVYDRMQFVMKATRSTFGQTYTST
QINPKTGETETIKHPVQNGSVVLDNQTGAVLGFVGGVSGELNHIYTLRSPGSTIKPLLVY
APAVDQKIIGSQTALADFKTDLGNNYSVTDYGGQIQNRFIPATEALAQSYNIPAVNLYKH
IKPTVNVKDYMDKMGIDTLTKNDYSQLGLALGGTDYGVDVKEQASAFSTFANQGEHVPAY
VISEIVDPSGHPIYQHKVKKTKVFSKGTTYIMNQMLKNVIDSGTAESLNYQLHFNTDNLI
GKTGTSNDFRDIWFIGSTPGVTIASWMGYDNNDGTTYSLDETGSMTNEAYWAKLTNAVYQ
TIPKQFKVNQKLARPDTVKSVTVDKQTGQPAGTLTFDGNTYRTGGSTVTSLYNDWQPTTK
NDFAIGGSKANYQLFFDHLDGKQNGYGQVSDANGNSTEAKKEHQETPITPTEDTQTTPSS
APSVTVPSSQQNQQGTSENQPSEPQTTGTGGTNGATGTGGTGGTGTTTETGTTTGTGTTT
GGGTNTGGTNNTNQQQTPPATTTGGGQ

Enzyme Prediction     

No EC number prediction in MGYG000002388_00857.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	123	300	1e-53	0.9322033898305084

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	3.22e-137	59	781	16	659	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.36e-129	125	720	3	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	7.96e-95	53	749	1	744	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG4953	PbpC	1.68e-73	126	696	60	540	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	6.74e-68	127	763	150	717	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF73751.1	0.0	1	927	1	927
QPC25600.1	0.0	1	927	1	927
QBA73687.1	0.0	1	927	1	927
AKU58922.1	0.0	1	927	1	927
QPC19905.1	0.0	1	927	1	927

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JE5_A	2.36e-136	71	793	1	709	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
2FFF_B	2.84e-63	337	793	2	444	OpenForm of a Class A Transpeptidase Domain [Streptococcus pneumoniae]
2XD1_A	4.09e-63	337	793	41	483	ACTIVESITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6],2XD1_B ACTIVE SITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6]
2BG1_A	4.09e-63	337	793	41	483	Activesite restructuring regulates ligand recognition in classA Penicillin-binding proteins (PBPs) [Streptococcus pneumoniae R6],2XD5_A Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6],2XD5_B Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6]
2UWX_A	2.00e-62	337	793	41	483	Activesite restructuring regulates ligand recognition in class A penicillin-binding proteins [Streptococcus pneumoniae R6]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	2.27e-68	74	802	31	672	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
A7GHV1	2.40e-64	75	749	31	669	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A7FY32	8.55e-64	75	749	31	669	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A5I6G4	8.55e-64	75	749	31	669	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A0A0H2ZMF9	1.77e-63	98	704	85	638	Penicillin-binding protein 2a OS=Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) OX=373153 GN=pbp2a PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999235	0.000771	0.000001	0.000000	0.000000	0.000001

TMHMM  Annotations     

start	end
56	78