CAZyme Information: MGYG000002396_00016

Basic Information

• Species: Bifidobacterium bifidum
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bifidum
• CAZyme ID: MGYG000002396_00016
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1292	MGYG000002396_1|CGC1	141998.56	3.9804

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002396	2311342	Isolate	South Korea	Asia

• Gene Location: Start: 18376; End: 22254 Strand: +

Full Sequence      

MFIPRYYESLGHLHVGTQPNRAYYVPASTPMDTVGENRVNSDRFMLLNGDWDFKYYASIY
DLDAEVSRLRAAGRPVFYDVDFGGDDAPENADPRTAATGALAADGFTTTPVPSVWQNHGF
DRHQYTNFDYPFPFDPPFVPQDNPCGVYLCDFMHTSDPDAPCTYLNFEGVDSAFYAWVNG
EFVGYSQVSHSTSEFDVTDVLEDGVNTLAVLVLKWCDGSYQEDQDKFRMSGIFRDVYLLD
RPEYAIRDMFVHTSIWRNVDSALVEAGISDDEYDASPVDHATVDVDFAFFDDADVPVKVQ
LFDEDGELVAETASEPIDDPMAGDEDDVTVRTQPDASEADDATGTGSDDTAADDEEEGVI
ESTDDVAAIDDDSTDAQAALRIASVTGTLAGGANGTGFTGDSAFAPTAHASLAVDDPHLW
TAETPYLYTIVYTTANEVITALVGIREVSVDGNVVKVNGKPIKLHGVNRHDSDPVTGPVI
SEEQLMRDLTLMKEHNVNAIRTSHYPNAPHFYDLYDRLGFYVVAEADNESHGAMRGVHPD
ESDAAVNKRWNRPIADNPAWIAPTVDRAQRNVERDKNHASIIFWSMGNECAYGCTFEAAL
LWTQTFDPSRLTHYESARYVDEGQECDYSYLDVHSRMYPSVEEIDQYFSEEGPRTPDGLR
DGSNGDDGDNGVKPYVLCEFCHAMGNGPGDLEDYFTRIQRYDGLAGGFIWEWCDHAIDRG
TNAAGKREYAYGGDSGEYPHFGNFCMDGLVYPDRTPHTGLLEFKNVYRPVRVTGFDAAAG
TVTLHNYLDFLDVADAVFMTFELLVDGVETAWAAWESDPAAGAAFREEHPGNYTPSMPSI
APHGDAVVDVPAEILDAIPEAGNVTMLVKYYQATDTETLPIGFELGFDEVAVPTADPRNQ
TVVAALADIADGIGSDDDVDDVADDGTDSVEGADDGANDAADAGYDDVVLADPLTVMQTD
ASITVEGSTFRYVLDRRTGLFSSMSFANRSLLNRPMELNVWRAPTDNDQYIKADWIRAQY
DRAQARAYEVGVLVDEDDVIAKPESIELRADDEDAPVASFGDDAILDDGNVDAGDVSAIA
TSEDGSVVVDGQVTIHATMALVAPIVQRIADIDADWTIAPDGSVALRMHVMRDTDFPFLP
RFGLRLFVPKPMRQIAYCGLGPNESYIDKRRSSYHGVFSGTPESLFEPYIKPQENGNHHD
CDWASVASDDTELLVLRAGDHAFDFQALPYTQEELTAKAHNSELKPADSTVVCVDYMQSG
VGSNSCGPKLHEKYRLDDAEFDFDLVLRPQAL

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	36	1174	7.8e-197	0.9694148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	0.0	7	1289	4	999	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	8.29e-179	17	1290	23	1027	beta-galactosidase.
COG3250	LacZ	2.27e-109	111	767	47	553	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	1.96e-94	448	770	1	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	2.20e-78	966	1287	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXM90753.1	0.0	1	1292	1	1292
ABE27151.2	0.0	1	1292	1	1291
ADO52393.1	0.0	1	1292	1	1292
ALE10600.1	0.0	1	1292	1	1294
BAQ97366.1	0.0	1	1292	1	1292

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6SD0_A	6.04e-136	3	1288	1	981	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
6S6Z_A	8.19e-136	16	1288	13	980	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6ETZ_A	1.63e-124	102	1291	64	989	Cold-adaptedbeta-D-galactosidase from Arthrobacter sp. 32cB [Arthrobacter sp. 32cB],6H1P_A Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB - data collected at room temperature [Arthrobacter sp. 32cB]
6SEB_A	2.60e-124	102	1291	85	1010	Cold-adaptedbeta-D-galactosidase from Arthrobacter sp. 32cB in complex with IPTG [Arthrobacter sp. 32cB],6SEC_A Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cBon complex with ONPG [Arthrobacter sp. 32cB],6SED_A Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB in complex with galactose [Arthrobacter sp. 32cB]
6ZJP_A	6.90e-124	102	1291	85	1010	Cold-adaptedbeta-D-galactosidase from Arthrobacter sp. 32cB mutant E517Q [Arthrobacter sp. 32cB]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P70753	6.10e-242	1	1286	1	998	Beta-galactosidase OS=Actinobacillus pleuropneumoniae OX=715 GN=lacZ PE=3 SV=1
O33815	3.55e-189	1	1288	1	992	Beta-galactosidase OS=Staphylococcus xylosus OX=1288 GN=lacZ PE=3 SV=1
Q56307	3.31e-135	3	1288	1	981	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	5.45e-133	22	1276	36	1019	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
P06864	3.56e-132	7	1289	4	999	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999963	0.000088	0.000003	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002396_00016.