CAZyme Information: MGYG000002396_01665

Basic Information

• Species: Bifidobacterium bifidum
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bifidum
• CAZyme ID: MGYG000002396_01665
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1808	MGYG000002396_1|CGC28	188782.52	4.8213

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002396	2311342	Isolate	South Korea	Asia

• Gene Location: Start: 1936928; End: 1942354 Strand: -

Full Sequence      

MIAASVALGTAFALIAPSSALAGDATPAAASTGTTYYVSSTHGDDGNAGTDKGHPWKTLD
KVNAIATDLKPGDSVLLERGSTFQDQYLHIKDTSGTADAPITIADYGEASAAKPVIAANG
VKGSQWYQNYRAHVGNHQNKGTVSSTILLKDVSYITVKNLEITNDDPDVYDPIDTWKWTD
TADSDGTKLDRSADRMDRTGVAGIAENGTTMSHVTLEGLNIHDVDGNIYNKHMANGGIYF
MAHYARENKSAADKTWLQNHISRFDHITIKNNIVKDVDRWGIAVGYTAYLNFIDATWGDG
SIDDDLIAKYGQTNVTIENNYVKGAGGDAITLMYCDRPVIQYNVGDSVSKHMNNIDYPHD
REHGGRYGGWVAAGIWPWRCKDPIFQYNEMYNNLNSEHGNGDGQAWDADYGDGTLYQYNY
SYGNSFASLMICNQYAINTTFRYNISQNDRRGVFDLPSNGPGNHIYNNTVYIGKDSAVLT
DRSNSQAKFENNIFINAADTKKTETWNRGSQHGGQTYDNNMYVNYANRPASDSHAVEVAD
VATVLANAGHAPSAPKANGSVYGRSGSEFDGYKPVDGSPAINAGKVISDMNDYAVTNDFF
GNTIKGTPDLGAVESNVLSVSGKVNAYETATVGGSKVVYVPFTAKNPTTVKQLRAGLTVG
DGSVANVYRGGNKLAADAAIQADDVLRFEVEGTSNTPIEYTIAQKNAWNWVDEFKTDSQG
PIWTSQRQVSGKWQTITDFGSDVYPQTYYDKYYGVGVDYQNKSLPADRSAIHGLIADNPG
DSAATAMAWTAPKAGSVKVTLDEVTGAHVEPYIRQSNSNGKDVVLKLLKNDEVICSATMT
DSFATATGFNECLASAKGTVKVAVGDVLRFSVDAAAGASKSSIHISPVITYQDVAPVESQ
TAQYAASYDAVSAKVGAKATATVKFTKGGAAATPTGVKSYALKTAVDGVAVDAATGAVTY
TPGADAYGTTKTATVVVTYSDDTTDEATVTFNVEKSNAQKYNVLYPAVSGGAGVALKRTP
KFTLKADSAAASIPAGTKFALGAGAPAGASVNAATGEVTLTSTRAGTITVPVTVTFSDTN
ESIEATATFTVTAPAALGTSKLETGTADGANVIYVPFTSKTPTTVADLLALVTAEPDSAL
KAVYRDGTKLEDAAAVKAGDVLRFYADGSTVTADYTVVQKNAWNWVDDFKTDSQGPIWTS
QRQVSGKWQTITDFGSDVYPQTYYDKYYGVGVDYQNKSLPADRSAIHGLIADNPGDSAAT
AMAWTAPKAGSVKVTLDEVTGAHVEPYIRQSNSNGKDVVLKLLKNDEVICSATMTDSFAT
ATGFNECLASAKGTVKVAVGDVLRFSVDAAAGASKSSIHISPVITYTDADEPSPEPGLSE
QYAAAYPASVAAKVGEKATAAVSFTKGGAAADAPAGTTFAVAGDGFTVAADGTVSFTPTD
AQAGKTVTATVTVTYSDKSTDTATVTFKVAAKTEPSPEPGLSEQYAAAYPASVAAKVGEK
ATAAVSFTKGGAAADAPAGTTFAVAGDGFTVAADGTVSFTPTDAQAGKTVTATVTVTYSD
KSTDTATVTFKVAAKTEPSPEPGLSEQYAAAYPASVAAKVGEKATAAVSFTKGGAAADAP
AGTTFAVAGDGFTVAADGTVSFTPTDAQAGKTVTATVTVTYSDKSTDTATVTFKVAAKDP
EPEPTDPKADLRKEVESASKLDEKDYTADSWKVFAAALDNAKAVLEDKDATEAQISGALT
TLKSATAALAKAGDTGKPDDGDKPSAGKPNDKGNGLSKTGASVAVIGVAMLLLAAAGFVT
VNVVRRRR

Enzyme Prediction     

EC	3.2.1.140

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	33	614	1e-184	0.9959266802443992

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam18957	RibLong	2.35e-10	998	1092	1	93	Long Rib domain. This entry represents the Long Rib domain that is closely related to the pfam08428 Rib domain but has a conserved insertion. These domains are found in bacterial cell surface proteins.
pfam18957	RibLong	5.94e-10	904	994	4	93	Long Rib domain. This entry represents the Long Rib domain that is closely related to the pfam08428 Rib domain but has a conserved insertion. These domains are found in bacterial cell surface proteins.
pfam13229	Beta_helix	6.76e-07	260	469	4	157	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam07554	FIVAR	0.002	1691	1749	3	68	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam13229	Beta_helix	0.003	384	499	35	139	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEG19581.1	0.0	1	1808	1	1807
BAQ97658.1	0.0	1	1808	1	1807
QTQ17888.1	0.0	1	1808	1	1704
BBA48469.1	0.0	1	1808	1	1808
BBA56588.1	0.0	1	1808	1	1809

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQC_A	0.0	19	615	3	597	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]
7V6I_A	3.13e-317	31	615	11	609	ChainA, Lacto-N-biosidase [Bifidobacterium saguini DSM 23967]
7V6M_A	2.97e-159	33	618	8	580	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
6KQT_A	1.83e-89	33	471	244	638	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]
6KQS_A	4.07e-85	33	471	244	638	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A3R0A696	2.04e-09	1689	1808	937	1064	Alpha-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=blArafA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000280	0.999020	0.000156	0.000199	0.000171	0.000152

TMHMM  Annotations     

start	end
1782	1804