CAZyme Information: MGYG000002403_03078

Basic Information

• Species: Robinsoniella peoriensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis
• CAZyme ID: MGYG000002403_03078
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1359	MGYG000002403_86|CGC2	148690.77	4.1783

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002403	7202103	Isolate	not provided	not provided

• Gene Location: Start: 28041; End: 32120 Strand: -

Full Sequence      

MRNKKRLKQTAAGMSALVMGASAILSAMAPIPVMAAGESKVAGEIAVNPDIHYQTLDGWG
TSLCWWGNIIGSWGDADWNGNGRADREEIAELAFSPEYLNLNIVRYNVGGGDKADSSIKR
CEGLVPGWTKDMTGTEDGSGEFNGDAFYAKSTEEMSDAGQLWMLEQANQYRYDYARETGE
ENTIINKVFSNSPPYYMTKSGSSTGGYWEDSNNLKDDYYDDFAMYMARSANWVDRNLKSK
YGVGVSYIEPLNEPDTNYWHDGSTKQEGCIFNTGELQSRAYREMQKALEAEEFQGNLNEM
KITGTDETNLWTSINSFNRLDDDIKANMDTISAHTYSGSDSERKELRQIAEKYDKGLWMS
EVTKGGGSHYEGCHESMDAANTRSQSEGIMADLKYMQPSAWIAWLVADSEYECLQTNSNW
GLIHAVFEKDGQPVPDYHTNLVDENGNRKEGVPGEGYWAVTKQFYTMMQYSKYLKAGYTM
IDIQEGNMCAAISPEGDELVIVAQNFDGERNTTVDLSAFPGAAAAELYRTSDSESCQLTE
TQDVTDGVLDMTLPSNSVSTYVIRANDKSAICSPSDYAKIVDADVEKPEEEWTSDINKFS
YAGSWGDSADDFGGGKYTTAQDGSADFTFEGSQALIYGTKAPEGALVNITVDGGEPQEVS
LVSADKEGSTILFNTGSLGEGTHTISISKAADQNEKLLEISCAKVVRGTLEEKVEKVEKS
KTVYTVSGVQPLLPAAVSAWTNMGNKVEKNVVWNLEGVDFTGNVTLEGTVPETSARASVE
VKVVTGNVQYFIDCNNPGSPEYTRVNSYAKLLNETPDQKLTNGTWGYLEEYGAYTGDKND
NYDTGWYAGQGQSISYVVPLKAGNYRVNLGFKEWWNQDRPMKVSVTAGDKTTELGTANTW
KGGNPWSEEVYEFTCETEENVTFSVAADGGPDPVLSYLRIQNILEVQELKEALAGAAAID
RSLYPMEKLAVLDDLVKAGHPLLYQAGTSQKDVEQAALHIRTALADLNGSGETEETDLTS
LRLAVAMAEKLEIQQNQNNCFTESSWAAAEEALNMAKAVLEKPDVSQEEVDLAFLKLITV
CNLLENDTQKTGLLATIEGAEAVLADEESLAQYTEESVQAVRNALAEAKRVYTNEGMNQE
EVNAAASKLMDAVTGLLLKREDSRLGILIQKAEAMLKNEAQYTPSTIEKLKTALEAAKAV
VKNSQASEAEINKAYNELAAAMTSLVRKGNKTELKNALDMADNMLQEPGKYLGSSIAGLQ
GLTDEARKVYDDVEADAERIGEVLKGLINEILKARLMGDTDLNGVVDTNDSAELLKYNAE
LRPFTEEQELVADVNGDGTADSGDAAVILQYAAEKIVSF

Enzyme Prediction     

No EC number prediction in MGYG000002403_03078.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	45	561	1e-124	0.9978070175438597

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14587	Glyco_hydr_30_2	1.89e-24	45	405	4	354	O-Glycosyl hydrolase family 30.
COG5520	XynC	9.45e-13	187	566	117	431	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].
cd14256	Dockerin_I	9.31e-10	1298	1351	2	55	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
cd14255	Dockerin_III	1.59e-07	1298	1354	1	62	Type III dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents the atypical type III dockerins and related domains.
cd14253	Dockerin	1.45e-06	1298	1353	1	56	Dockerin repeat domain. Dockerins are modules in the cellulosome complex that often anchor catalytic subunits by binding to cohesin domains of scaffolding proteins. Three types of dockerins and their corresponding cohesin have been described in the literature. This alignment models two consecutive dockerin repeats, the functional unit.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWY99102.1	0.0	1	1332	1	1332
QIB57138.1	0.0	1	1359	1	1347
QMW80085.1	0.0	1	1359	1	1347
ADL05173.1	8.11e-285	38	1020	37	1270
QJU15030.1	6.71e-124	118	1015	302	1052

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CLW_A	3.38e-16	53	567	16	500	Crystalstructure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_B Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_C Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_D Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_E Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_F Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A401ETL2	2.79e-29	45	535	37	586	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1
Q76FP5	1.39e-21	45	517	24	421	Endo-beta-1,6-galactanase OS=Hypocrea rufa OX=5547 GN=6GAL PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004002	0.994016	0.000401	0.000702	0.000446	0.000398

TMHMM  Annotations     

start	end
13	35