CAZyme Information: MGYG000002403_05081

Basic Information

• Species: Robinsoniella peoriensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis
• CAZyme ID: MGYG000002403_05081
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2186	MGYG000002403_132|CGC7	239216.66	4.7396

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002403	7202103	Isolate	not provided	not provided

• Gene Location: Start: 269459; End: 276019 Strand: -

Full Sequence      

MGKRRKKLLSMALVVGMVVSNLSPYTNVLAAEPDNVSTGSAETAELNEDPGDALPPGTAK
AIKGTESETEPETEPVSRVETETEAVRESTSQAETIAETNQVETEKETMSETETQLTEET
ETETARETKNKNAAAVNNVDVNGLFAEYYTTSGSGKNVKLDALKSKGIDYNINYGDLNAK
LMLTTGKDDYAGIRWSGRIRVPETADYTFYGLADNGIRLWVNGEQLFNYWDGESWDILQT
SKGVALEAGKFYDIKVEYFDYSGGAHATLSWSNNKGLKDKSTIPSSAFFLPSDYNGIYIG
SIDTSQANLKEGEDFKGDVTVNGFGLDQVESFEIVKTSGESLKTPVYAEVTSQKNEEAKI
VIPNMETGTYKLKIKQSNTAVISKGLIIVKPGTEEVPTRTERPRADWERESYVNLNGIWE
FDFDADEEGKNAGWYNPDQEFSRSINVPFCWESSLSGISDPDYKGQAWYKKTVRVDKSWE
GRKIFLKFGAVDWKCKLWVNGEEVGEHIGGYSAFEMDVTDHMKAGEDNVITLWVEDKGSY
GDDSYPALIGKQGRNAPCGYIHTSGIWQTVGMEARSATYLDNAKAVSDIDNATVTYDLDV
TTDKDQTLTVEYDFASTVYDMENDVDIPTGSVVEGSQEIQVESGDNEVELSPIAIANQKL
WNYDDPNLYQGTLTVKDMDGNVLDELSTYFGLRKVEAKYYNEDLGVKYIYLNDEPVYMSG
LLDQGFWEEGIYTAPSEDALKYDILAMKEAGFNMIRKHLKIEDPLQYYWCDKLGMMVWQD
MPHATAMVPSKEGGEALGRKYYEECLDAAMEMNYNHPSIVSVMLFNETWGLYAAYNDNGK
NRNVKASDGKSTAEWVEYLYNKTKGAYPNMLIEDMSPCNSDHVQPSDLNTYHMYPKSYDG
TVGDVENRVNNAYVGSENNYKFGFKQDGDPLLNSEYGGVAAYDGDFDISYCFKFMTDVQR
RYEKQSGFVYTEPFDVEYERNGILTYDRQKKIFGYDEIAYGGDMGIQDLVQETYIGIVDS
PVKNVKPGAKIKTKIMAMSWTNDVPENTVVKWRFDGTDIYGNNISTDLAGTLGMEIIPYE
KAEASLSFHAPAQACVGTLTVWIEGPDGAKIAKNFTNIVVADPSSENKELILDNENGSIT
MKAAVNDRRMVTTEGTGSQDYSYTLPENFNLDTLNGLKIIAEASSFKGQTGTDKNHSSFS
SAYSQTAEGRERASDMTVYVNGVEVDTVYLPDNPRDMRGTLSLNAPYNGATSAGDFGYLV
NLSVSKEKLAEIKAAMGDSKVMKVTYAVKEDAANQNGLRIYNSVYGRYAVNPTIILNPKD
IEKADQVTAVKNIKTDSDNYSVEGVLSGNSSLNVRTGEKEGYVIALANGGSSITVTNKKT
NQVIGDAAELAAGNHHVKVTLFDEQIRVFADNNPEAAINVFDRSGFTGGITVNASGTNPV
ADLVVSPESYEAVKSEIDDTVKDVEVTDDFSDADYAKRYEIMGGAWSGNVLNGALNMSAS
DQGDKMIMKGISMSDGIYEMDIKVTNSSGINGNAGFLFRSSNYNIGPDGANGYYAGIGDR
YVQLGRMSQGWKELAKVTVPELVVGSTHTLRIAVFGSRIQIYVDDAQVPCVDINDSTYLE
GGAAVRGYRVAATLDNIHVASIPRYSSTFEKGSDEWEANGNWKTVDGAYTSASKGAYSLI
DSNKIKDVLFAADVKPGTEDSVTAMLLRTVSGSAGIRGYQLVADAENDKVQIVKSENGRT
TVLAETGMRLQAGRTYRLTVEMQGSVIKAYKNDNREALMIAEDSAFTAGQIGIMNVNGTS
AIDNVAVNNQFIDGGLMKPADPKALDEIIAEAQKADAGKYTADSYSRLKAALDAALDADR
YDQQEIDGAVTAVREALNQLVLKPVDLTVLNSKIAEAKKLDPGRYTADSYAKVKAALAAA
EAVNKKDQAAVDAAAKALGDAIGQLKIKPADLSVLNQKIAEAKKLDVNKYTADSYAKVKA
ALAAAEAVNKNDQAAVNAAAKNLSDAMAQLKVKPAAPKVPKKNAVYTVNSLVYKVTKSSS
KNGTVKVMKAKKKSYTSISVPKTVKLNGYTFKVTEIYKNAFKNNTKLKTVKIADNVSKIG
SYAFAGCKKLKSVTIGKGLKSIGSKAFYNDKVLKSIVIKSTKVSKVYSKAFSGIHKNAYI
NVPNKKASDYKKTFKKGNPAKTVKIK

Enzyme Prediction     

No EC number prediction in MGYG000002403_05081.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	389	867	6e-96	0.5
CBM66	1483	1636	6.8e-24	0.9290322580645162
CBM66	1656	1805	1.9e-21	0.9612903225806452

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.15e-40	415	844	15	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.91e-33	402	827	3	417	beta-D-glucuronidase; Provisional
sd00036	LRR_3	3.10e-24	2053	2165	14	139	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.
pfam02837	Glyco_hydro_2_N	5.41e-24	415	570	4	163	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
sd00036	LRR_3	2.61e-23	2072	2176	1	104	leucine-rich repeats. A leucine-rich repeat (LRR) is a structural protein motif of 20-30 amino acids that is unusually rich in the hydrophobic amino acid leucine. The conserved eleven-residue sequence motif (LxxLxLxxN/CxL) within the LRRs corresponds to the beta-strand and adjacent loop regions, whereas the remaining parts of the repeats are variable. LRRs fold together to form a solenoid protein domain, termed leucine-rich repeat domain. Leucine-rich repeats are usually involved in protein-protein interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIQ74895.1	2.56e-133	393	1311	10	911
AWV34213.1	2.22e-132	399	1311	15	911
AIQ24463.1	6.85e-126	399	1311	10	906
QGQ94319.1	1.11e-124	413	1311	29	907
AIQ36271.1	2.29e-123	399	1311	10	906

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.20e-64	401	997	28	572	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5Z1A_A	3.79e-23	403	827	16	414	Thecrystal structure of Bacteroides fragilis beta-glucuronidase in complex with uronic isofagomine [Bacteroides fragilis NCTC 9343]
5N6U_A	5.46e-23	413	835	27	457	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
3CMG_A	1.10e-22	413	827	7	395	Crystalstructure of putative beta-galactosidase from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]
6LEM_B	5.91e-22	415	827	13	411	ChainB, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPJ7	8.35e-24	443	831	80	434	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P05804	3.27e-21	415	827	15	413	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
T2KM09	1.29e-18	399	828	41	433	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	1.91e-17	458	819	49	379	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
A3DC75	9.70e-16	136	293	351	496	Anti-sigma-I factor RsgI3 OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=rsgI3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000329	0.998934	0.000210	0.000196	0.000176	0.000150

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002403_05081.