CAZyme Information: MGYG000002403_05460

Basic Information

• Species: Robinsoniella peoriensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella peoriensis
• CAZyme ID: MGYG000002403_05460
• CAZy Family: GH29
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2029	MGYG000002403_140|CGC2	219126.09	4.5958

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002403	7202103	Isolate	not provided	not provided

• Gene Location: Start: 66682; End: 72771 Strand: -

Full Sequence      

MKKTWKRFVSTAMTAAMIVPGVLAPVSSMSAKAETREVNREGVYSIDLSEKGTEAPAPWG
VLPSQNQYDYQKQELAAFCHFGMNTYTNTEWGNGQESPSSFALENDFDADNMVKSLQDAG
FKKLIITAKHHDGFCIWRSKYTEHDLESTDYEGDVLKEISAACTKYDMDMGLYLSPWDVN
NPSYGYRDEQGNPTDKEHDFLDYNEYYNNQLQEILGNNEYGNDGHFVEVWMDGAKGSGSM
AQDYDFDLWFDTIQKNEGVASKNFESDCMLFGAQSRTTVRWIGNESGFANEETWSKSRVD
KERDTIDSRQVGGATSGYHDGNQWTVPEVDAKITSGWFWGPGKKTPLSLEQLSNMYFDSV
GHGAVLLLNVPPNREGTIDQTILDRVSEFGSNINETFKKNLAAQEGVTISASSVRGDDIA
YSPDNLKDNNDDTYWTMNDGSTTGSITIDLGSTKTFDVVSIEEAIKLGQRVSSFTVEYQN
QGGEWKKFAEGTTIGPKKLCRKTPVKADKVRINITGSYAVPVISEVGIYKASQGFEIGQA
IPDGLDNIDIKDTDTTDGIGFEIGNGWTQETGSQYTNGTNMWANPNAELTLKFKGTKAWL
LGTQDPNHGTADLYIDGAAEPVTINTNASKRAVGQVLYETPDLEDGQHTIRLVVKNKAIG
LEAALALNNGGKGMFELEQTAYTVPEDTENAFVVKRIGGSKGRATVLFQDNPGTAVQSEY
IPTEGIELVFEEGETEKTAHVTTKRQTLNTGDLYFSVDLVEPSDGAVLGFKPSARVTITD
ADKITKDMVSELIASTDSLVSAYYKEDSWNAMMDAKKEAQKVVDNESPTGVAITNAYNNL
KAAIDGLVSRDPYTEEDPFVFPAYKDQSKPLEAEFFALDPIEGNKYVRITESDQASNGKE
VNWFEPGNKIILHYTAEKAGTYNLEATYRSGRAQNNPNAFVWSGDKIESGSQDVYGEDGA
TEYHKVVLPIKITEAGAGTLIFTADAKAGPVIDKFEITPNEIDYVKFQIDASAGEHGSIS
DAGDNEVYQGTDKKFVITPEANYKIDDVLVNGESVKDQLVVEDEAANSYSYTFVNVQANS
TIEASFVFDHYTEALPFAFPSDDSTVNLEAEHFTLYPVNTDKYVRISDKANASNGKEINW
FETGNVIKLPFTAAQAGTYTLTATYRSGRNSSAPNAFVWSGTNVVSGSQDVYGPDANVYK
TVALPIVVTKAGAGELAFTADAKAGPVIDKFDVQFTAPAAPVESVELDKTSATLTQVGET
LQLTATVAPENATNKNVTWESSNPEAATVDENGLVTAVATGTANITVTTEDGNKTASCEV
TVGLRVTGIAFDVTEKTLTASGETFQLNPVFTPQDAMNKELTWRSTNESAATVDQSGLVT
AAANGNTEIVAITKDGGYVAACRVNVEIPVRAESVSLDRTTAEITTENGNIQLTATVNPE
NATNKNVSWKSSDPDIASVDGNGLVTAVSDGPATITVTTEDGGFTAVCEVTVAIVPEVIP
VEGITMDITEHTMQKAGEILQLKPQVQPSNASNQKITYNSSNPEAATVDENGCVTAIANG
DAVITATTEDGGFEAACTIKVAIPVGVTELILDKKEATLTKAGETLELHETVMPENATNK
NVTWISTMPEVADVDQGVVTAAADGTTTIIALTEDGHFTATCQITVSIVKPVTGISLKET
HLKFSKAGETAQLKAAIAPEDATNKNVAWTSSDSKVAEVDKNGVVTAIADGKAIITVKSE
DGAFMASCEAEVKIDKPVISVTGVSLNKTNAVLYGKGASLQLKAEVYPENAANKKVTWTS
KDKKVAKVDSNGKVTAVSNGKTEIIVKTADGNITAKCAITVKEDNKPAVVKPGRVTNVKV
SGNATHSMKVTWNKVKDADTYRVYVYNQKFKKWSKAGTTNETGMTIKRLPVGMECQVKVA
AVNKAGKGAYSTAVTTATRPNKVTLKSVKKNTADSVKLTFKNVRSDGFAVYMKAGKGKYK
KVDTAKTTTAVVNKLKKGTTYSFKVRAYVKADGKTFYGKYSNVITYKMK

Enzyme Prediction     

No EC number prediction in MGYG000002403_05460.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	63	393	2e-58	0.869942196531792

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3669	AfuC	6.76e-67	59	529	2	430	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
pfam01120	Alpha_L_fucos	6.83e-26	106	394	83	331	Alpha-L-fucosidase.
COG5492	YjdB	1.23e-25	1241	1396	180	324	Uncharacterized conserved protein YjdB, contains Ig-like domain [General function prediction only].
smart00812	Alpha_L_fucos	2.33e-25	106	391	80	328	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
COG5492	YjdB	6.13e-20	1264	1571	22	324	Uncharacterized conserved protein YjdB, contains Ig-like domain [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT29448.1	0.0	2	1242	11	1251
QHB23055.1	0.0	2	1242	11	1251
QEI30547.1	0.0	2	1242	11	1251
PLT74914.1	0.0	2	1242	11	1247
QPK81604.1	3.93e-292	24	996	20	1001

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6TR3_A	1.32e-213	54	578	19	552	Ruminococcusgnavus GH29 fucosidase E1_10125 in complex with fucose [[Ruminococcus] gnavus E1]
6TR4_A	1.91e-212	54	578	19	552	Ruminococcusgnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1],6TR4_B Ruminococcus gnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1]
6ORG_A	9.30e-107	58	530	6	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORG_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6OR4_A	1.06e-105	58	530	6	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6OR4_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_A Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6ORF_A	1.10e-105	58	530	6	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORF_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GW72	8.69e-83	56	529	31	477	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2
Q7XUR3	2.37e-77	44	532	21	479	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2
P85991	3.45e-17	1688	1841	105	257	Ig-like virion protein OS=Serratia phage KSP90 OX=552528 PE=1 SV=2
P33747	7.55e-16	1257	1406	51	201	Uncharacterized protein CA_P0160 OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=CA_P0160 PE=3 SV=2
Q086E4	1.43e-12	1276	1855	79	665	Ice-binding protein 1 OS=Shewanella frigidimarina (strain NCIMB 400) OX=318167 GN=Sfri_1018 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000734	0.997825	0.000373	0.000600	0.000241	0.000199

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002403_05460.