CAZyme Information: MGYG000002453_00386

Basic Information

• Species: Akkermansia muciniphila_B
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Verrucomicrobiales; Akkermansiaceae; Akkermansia; Akkermansia muciniphila_B
• CAZyme ID: MGYG000002453_00386
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
888		98427.87	9.7418

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002453	3096868	Isolate	China	Asia

• Gene Location: Start: 79055; End: 81721 Strand: -

Full Sequence      

MGLNDKSRKRNAGSWRERIQDGEAASRPQTERKKRPAPRPSYPGLPQEDPGPAVIPGPTR
RTRNSRSGSSRHDTPPPRPRRAARPAPQEEYEDYPEAEGESRSARAGRPRKPRFTIFGAL
LFVLLMPFRFIGSLTRNIRWFISWPLRILLGCCFVGIVIGSILVFLYGTISNRYDISEVK
SNIPERTVILDRKNRTIGTLHGENRKRVFLREVPPIFIDALILREDNRFYDHGGVDWIGV
GRAFAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILE
TYINRIFWGHTFLGIAAAARGYYDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKKV
RDIVLGLLKNEGKITEVQYQAALAEPIVTRTPQSRSEENYAMDLVRRELDAILEEEDIRL
GGLVVHTTLDLDLQNATLDAINKHMEALEARKDFKKHLASLQAKREKKGILKNKLTTKAE
YEAALAAWKALPAERKEGMQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEESKLNRAIQ
SRRQTGSLFKPFIYATFFQQGNSADTRISDDRIAYGEVRGAANWSPRNSDGTYRGMKPAS
FGLILSRNTMSVRIGNRAGLSNVIQSAQLAGFHGNISRTPALYLGTWEASPLDIASAYSV
FANGGVRPTPYIIDHITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGRI
TALGFKSPCGGKTGTTNNYTNAWFAGYTSNLTCSVWVGFDSSTKILEKGYGGTLALPVWV
DIMLAAQKEGYPANAIRTRPGSEGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPTR
MCEKHIPMAEPDTEENIPYAEPLDGSDDNIPLAEPVEETDAIPYATPI

Enzyme Prediction     

No EC number prediction in MGYG000002453_00386.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	195	366	8.5e-58	0.9661016949152542

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	4.27e-167	204	788	1	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	1.33e-163	134	816	7	626	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.22e-158	141	862	4	788	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	8.78e-108	195	783	134	679	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
TIGR02073	PBP_1c	7.41e-94	184	847	9	595	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWP53885.1	0.0	1	888	1	888
QWP48975.1	0.0	1	888	1	888
QWP03195.1	0.0	1	888	1	888
QWP61070.1	0.0	1	888	1	888
QWP68404.1	0.0	1	888	1	888

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.14e-90	194	797	28	725	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	2.26e-88	202	843	35	773	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	7.15e-69	177	780	1	751	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
5FGZ_A	3.00e-60	197	776	148	685	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]
3VMA_A	4.01e-60	197	776	169	706	CrystalStructure of the Full-Length Transglycosylase PBP1b from Escherichia coli [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O87579	2.68e-91	159	801	21	719	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
O87626	5.16e-91	155	788	21	706	Penicillin-binding protein 1A OS=Neisseria flavescens OX=484 GN=mrcA PE=3 SV=1
Q5FAC7	6.97e-89	152	788	17	707	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) OX=242231 GN=mrcA PE=3 SV=3
O05131	6.97e-89	152	788	17	707	Penicillin-binding protein 1A OS=Neisseria gonorrhoeae OX=485 GN=mrcA PE=1 SV=3
P0A0Z6	9.47e-88	152	788	17	707	Penicillin-binding protein 1A OS=Neisseria meningitidis serogroup B (strain MC58) OX=122586 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000060	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
114	131
146	168
301	323