CAZyme Information: MGYG000002453_02159

Basic Information

• Species: Akkermansia muciniphila_B
• Lineage: Bacteria; Verrucomicrobiota; Verrucomicrobiae; Verrucomicrobiales; Akkermansiaceae; Akkermansia; Akkermansia muciniphila_B
• CAZyme ID: MGYG000002453_02159
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1289		142822.02	9.0797

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002453	3096868	Isolate	China	Asia

• Gene Location: Start: 611658; End: 615527 Strand: +

Full Sequence      

MKTVRAFLFSVMCLAPALPLLGEAPDWENEEVTGINKEAPRASVLPAGDKTLSLNGDWKF
KFSMTPDGRPADFFKPSYSVSGWDTVKVPSNWQLAGYGTAIYTNVPYPFKPNPPKVMGEP
PRNWPAYKERNSVGSYRRDFNLPASWKGEQVMIRFDGVESAFYVWVNGRKVGYSEDSYTG
GEFDLTPYVKPGRNTIAVEVYRWSDGSYLEDQDFLRLSGIFRDVTLFAQPQVHVRDLFLK
AGLDRKDYTTGVLDGTFTIRNSGKKDIPAGMKLDYSIEGISTNLNWEGASGSGRVQTDNR
GRLDSGTLEVPAIPAGGEVQVALDRKFPGVKPWTAETPNLYKVTYSLNGKDPRSVNIGFR
SVEIADNGAVLVNGKAVKFKGVNRHEAHPDYGRAIPREVMEKDVQIIKAHNINTVRCSHY
PNHPYFYELCDRYGLYVMDEANCEAHGIRNSGMDISRKPSWKKAHVERNMSMVHRSKNHP
SIVFWSLGNESGNGPNFEAASEAIRAYDDTRPLHYCEFPHGHKAVDMDSAMYPPVDRVEN
WGKQKTSRPFFVCEYAHSMGNALGNFKEYMEAFESSPRMVGGAIWDFVDQSLRANPAGNG
IYKPAPFKGVTQAYGGMFGDRPNQANFCDNGIILGNRNTTAKTKEVKKVYQYMAFDRKDG
SLTVRNKYFHKPLKGYTLYLVSLAPGGKHAVERMTLPEAAPGKSVTVKLPSAAMQTGSLM
VLADARFKLPEDVKELSAKQLEHVADECEAYEWFPAAMEKEASVKAPAALPAVSVRQGDP
VVVQGKGFSASFKDGMLSALRYGSQDLILPGYPVVLQAYRSPVDNDAWIRSKVEGKMKMQ
NMKAEYSDVKAAVISPGVARITAQFRTKGSDLSFSGEVAWTVFGNGIINASVRMYPSAKG
EELLRLGVTFGMPAAYDQVEYLGLGPWDNYRDRRTSCWKDVFRTSVDDMFFAYSRPQDMG
NRMETDWVALSRPKAAPALWVGSASPRAPLEVSILRYTPKEFNNAKSLDRLPEKDKVVVN
LDAFQMGLGGSSCGPRPLAKYQTLSEATPLGFVLAPTSALLNLARAGLGVPHSPVIERDG
DGMVSLVSSTPGAEMKYSVNKGPEKTYRKPFKLPEGEVRAWAAAGKSGKVPPTSPGERKF
PLVKGQGEWKILSASSEEPDTGFAHFAIDGNPDTCWHTSYTNGLPGFPHSIAVDMGTRMK
FTGFIYTPRMDKDKGLISQYTFSVSDDGQNWTEVKKGQFTYHYIRKDPAVQRIDFGKPVE
ARYFKLDARSPVKGGEQSATVAELNIITQ

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	48	938	1.3e-209	0.9627659574468085
CBM32	1154	1284	1.3e-22	0.9112903225806451

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	0.0	27	1059	4	1002	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	0.0	26	1030	16	999	beta-galactosidase.
COG3250	LacZ	1.22e-145	53	936	14	803	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	8.74e-94	362	655	1	302	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
smart01038	Bgal_small_N	1.15e-62	784	1054	1	272	Beta galactosidase small chain. This domain comprises the small chain of dimeric beta-galactosidases EC:3.2.1.23. This domain is also found in single chain beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWP48028.1	0.0	1	1289	1	1289
QWP62702.1	0.0	1	1289	1	1289
QWP20951.1	0.0	1	1289	1	1289
QWP60157.1	0.0	1	1289	1	1289
QWP26183.1	0.0	1	1289	1	1289

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	1.63e-201	26	1115	3	1055	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	1.67e-201	26	1115	4	1056	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
3DEC_A	6.62e-175	23	1041	3	981	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
3BGA_A	4.95e-173	25	1056	8	1000	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3MUY_1	1.82e-158	19	1042	8	1008	Chain1, Beta-D-galactosidase [Escherichia coli K-12],3MUY_2 Chain 2, Beta-D-galactosidase [Escherichia coli K-12],3MUY_3 Chain 3, Beta-D-galactosidase [Escherichia coli K-12],3MUY_4 Chain 4, Beta-D-galactosidase [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56307	9.16e-201	26	1115	4	1056	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2
O52847	1.80e-179	25	1030	18	1005	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
Q32JB6	6.80e-160	14	1042	4	1009	Beta-galactosidase OS=Shigella dysenteriae serotype 1 (strain Sd197) OX=300267 GN=lacZ PE=3 SV=2
A7ZI91	2.69e-158	14	1042	4	1009	Beta-galactosidase OS=Escherichia coli O139:H28 (strain E24377A / ETEC) OX=331111 GN=lacZ PE=3 SV=1
A7ZWZ1	2.69e-158	14	1042	4	1009	Beta-galactosidase OS=Escherichia coli O9:H4 (strain HS) OX=331112 GN=lacZ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000329	0.998835	0.000265	0.000202	0.000176	0.000161

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002453_02159.