CAZyme Information: MGYG000002455_01392

Basic Information

• Species: Bacteroides cellulosilyticus
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides cellulosilyticus
• CAZyme ID: MGYG000002455_01392
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1588	MGYG000002455_5|CGC3	178557.59	7.9704

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002455	6974062	Isolate	United States	North America

• Gene Location: Start: 138304; End: 143070 Strand: +

Full Sequence      

MFMKCLKLIFCLLYLCCSLAEAKEVQVDAQKEVDEMTGRLVASYLEAKVDEDIIAGYASA
LRSDGSFPDLDYVTVHEGSSYPAGSHLKRLKLMAIAYRKPGNKYYNSGRLLKQIVAGIDY
WYRVRPKSGNWWFGDIGAPQDYMVPLILLKGKISDKKLLHYSSYLQDLTGNKGHKGKNRT
WVSAVTIHKGCIENNIELIRIGIKSIASTIKIVPEQGDEGIKVDGSFHQHRPQLYSGGYG
LSYVDDIAYYLQLVKGTAFESYFTQEKKDIFINLLMGGHRLLGYRETFDFGAIGRNISRP
EGLSNISPVTLEYMEQNDSDRAADYSAWKKHLSGAPFPAPGNKYFWKSDIMVQHGTGYYL
SAKVISTRTNGTEMLNNENLKGYNLPLGATNILTSGKEYEGIFPVWNWNKIPGTTAVQHQ
DSTRLEGYLFGKNRFGGGVSNGKNGVIAYEHCYKGVKARKSYFFMNDVLLCLGTDIASDA
PEEVVTTVNQCLFTGEMVVGKEEGTMSVYRENVSVKNPAWVYHDKVGYLFPSGGDVIVSN
PKQTGAWKDINISGSGKKISADIFNLWISHGVKAKEGKYAYMVVPDKSLEEFRTFTATQN
YKIIQNSSVVQAVKLNQQYAIVFYHPGTIDLGEGLTLATDKQVIVYLEQKGTGYDIWVAD
PLYSQREVCLALNGREVQIAFPEGELTGSTAFTNIATLQPFDLQCEYLSNPLGVDILQPR
LSWKMGATTSARGRKQTAYQILVASSRDLLDADRGDLWDSGRVNSAESVNIVYGGVPLSA
GQRCFWKVRFSDEHNRWSAWSNPANWRMGLFAADWAAQWIGSAEMESQSVGGKKVNNVMA
DPWFRKTFNMSDIPQDAVIYVASIGYHELYVNGRKVGDAVLSPSVTDHKSRARYMTYDIK
GYLKTGTNVIALWLGTSWAVFPAYQQKNRPAIPMALVQAEIALSSGKKLRIVSDNTWKTH
ASPNTLLGYWEAHHFEGECYDAALEKDGWNTPDFDDSGWAMAKVYSTDVIVSSDRTEPNR
LLGEIKPLSVQEVSPGVYRVDMGINYAGWFEMQLQGQPGDSIVFQFSEREKDACSYGIHS
IYKVGSKRKGVFCNRFNYMTGRWVQITGLRYKPELDQIRGWMIRPDYRRSGGFECDLPLL
NDIYRTTLWTFENLSLGNYVVDCPHRERCGYGGDALATTRTALGNYQLGAFYSKWMEDWR
DVQDPEGNVPYTAPTRIGGGGPSWSGFCITLPWELYRQYGDVRILSESFPTIQRWLSFVE
TKSQDDMLVRWGGKWSFLGDWLWPDAWSERSAMEKQGKALGDTRETLFFNNCHWIYSLET
AAQIADVLGNRAAASAYRNRASKVRKAVHATFFNSNDNSYVNGYPSYLAIALMVDLPPKH
LQAKVWKRLEQEVLVKRKGHFWGGITAGSFLLHTLLDNHRNDLIFEMAMKEDFPGWGDML
KHGNGTFFEDWQCRGSGLHSSYLYIGSWFIEALGGIRRPEAGYKEFSIEPWITKGGPKQV
RSYYNSLYGKIVSDWTLEAGVLKMEITIPANTTAVLKLSGIHPATLKEGETAWKEAEGVS
LYSQKRNALSLALQSGTYRFSVVMDEGR

Enzyme Prediction     

No EC number prediction in MGYG000002455_01392.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	1031	1538	3.6e-122	0.996031746031746
PL8	343	586	3e-80	0.9879518072289156
CBM67	817	1006	1.4e-34	0.9545454545454546

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	1.47e-123	31	661	8	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	7.63e-87	342	588	1	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam17389	Bac_rhamnosid6H	2.18e-70	1128	1473	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	5.17e-40	854	1020	3	169	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam08124	Lyase_8_N	2.50e-31	47	304	26	291	Polysaccharide lyase family 8, N terminal alpha-helical domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT88602.1	0.0	3	1588	1	1586
ALJ60391.1	0.0	1	1588	1	1588
QUT74996.1	0.0	698	1585	33	920
BCI64727.1	0.0	662	1585	7	925
QDU88571.1	4.15e-293	703	1586	798	1691

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6I60_A	1.21e-111	682	1580	16	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
3W5M_A	3.33e-90	706	1582	12	1030	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
1CB8_A	1.63e-85	59	690	33	674	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	2.71e-85	59	690	55	696	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
6GSZ_A	1.58e-71	706	1540	10	848	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KNB2	8.14e-103	706	1580	42	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
T2KPL4	1.42e-98	709	1588	41	944	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
Q82PP4	1.49e-89	706	1582	12	1030	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
Q59288	1.48e-84	59	690	55	696	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
P9WF03	1.25e-81	706	1586	38	914	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000197	0.999199	0.000196	0.000147	0.000136	0.000130

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002455_01392.