CAZyme Information: MGYG000002475_03403

Basic Information

• Species: Lactonifactor longoviformis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lactonifactor; Lactonifactor longoviformis
• CAZyme ID: MGYG000002475_03403
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase large subunit

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
628	MGYG000002475_297|CGC1	73049.12	4.9334

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002475	4812037	Isolate	Germany	Europe

• Gene Location: Start: 1738; End: 3624 Strand: +

Full Sequence      

MEADIRWIDNPEVFRVNQLPAHSDHEFYHTTEELLHGKSSLVQSLDGQWQFHYSINASVR
PVSFYKKDFNAADFDWITVPGHIELAGYDKNHYINTMYPWEGHEFRRPAFSMEAGNTGEG
SFSSASWNPVGSYLCRFDLKEGMRGMRINIRFEGVEQAMYVWLNGAFIGYAEDSFTPSEF
DLSPYIEETGNILAVEVHKRSTAAFLEDQDFFRFFGIFRSVSLYARPGIHLEDMWAKPFY
RVEEGVGELELELKLSCIKQGGTVRAVLQDEEGHICMEQEETIKEKMKLVSDGLGKVTPW
EYGFPYLYTLLLELRDADGNIQEIVPYKIGFRTIEICGRVIFLNGRRLILNGVNRHEWSA
RRGRAVTEEEMDWDIRCMQRNHINAVRTCHYPNQIPWYAKCDKAGIYIMSETNLESHGSW
QKMGAVEPSWNVPGSIPQWREAVLDRARTHFETFKNHVSIIFWSLGNESYVGENIKLMQD
FFRQSDPTRLIHYEGVFHNRVYGDAISDVESQMYAPPGRIREYLEHNPSKPFILCEYMHN
MGNSLGGLKSYMELLDQYEMYQGGFIWDFIDQALWTEDEVTGEAVLRYGGDFDDRPSDYE
FSANGIVFADRTEKPAMQEVRYYYGKYR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	24	625	7.1e-140	0.6329787234042553

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10340	ebgA	6.14e-150	6	621	3	596	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.75e-138	10	622	19	623	beta-galactosidase.
pfam02836	Glyco_hydro_2_C	2.04e-131	334	626	1	299	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
COG3250	LacZ	1.76e-116	39	625	9	554	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	3.66e-30	137	590	73	569	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNM02889.1	3.17e-317	1	625	1	633
ABX42393.1	4.90e-316	1	627	1	638
QEH67710.1	5.72e-302	1	628	1	626
AQP40966.1	3.00e-301	1	628	1	633
VCV22603.1	1.63e-298	1	628	1	633

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6S6Z_A	4.71e-117	44	624	40	593	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	4.80e-117	44	624	41	594	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]
6CVM_A	5.66e-106	9	622	16	621	Atomicresolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_B Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_C Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6CVM_D Atomic resolution cryo-EM structure of beta-galactosidase [Escherichia coli K-12],6X1Q_A 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_B 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_C 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12],6X1Q_D 1.8 Angstrom resolution structure of b-galactosidase with a 200 kV cryoARM electron microscope [Escherichia coli K-12]
1F4A_A	7.88e-106	9	622	15	620	E.COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_B E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_C E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4A_D E. COLI (LACZ) BETA-GALACTOSIDASE (NCS CONSTRAINED MONOMER-ORTHORHOMBIC) [Escherichia coli],1F4H_A E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_B E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_C E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli],1F4H_D E. COLI (LACZ) BETA-GALACTOSIDASE (ORTHORHOMBIC) [Escherichia coli]
5A1A_A	8.02e-106	9	622	16	621	2.2A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_B 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_C 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12],5A1A_D 2.2 A resolution cryo-EM structure of beta-galactosidase in complex with a cell-permeant inhibitor [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q48846	1.18e-292	1	628	1	625	Beta-galactosidase large subunit OS=Latilactobacillus sakei OX=1599 GN=lacL PE=3 SV=1
Q7WTB4	2.30e-281	1	625	1	625	Beta-galactosidase large subunit OS=Lactobacillus helveticus OX=1587 GN=lacL PE=2 SV=1
Q02603	4.53e-271	1	625	1	623	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1
O07684	1.39e-270	1	625	1	625	Beta-galactosidase large subunit OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=lacL PE=3 SV=2
P24131	6.10e-221	2	624	5	612	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002475_03403.