CAZyme Information: MGYG000002478_04580

Basic Information

• Species: Phocaeicola dorei
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola dorei
• CAZyme ID: MGYG000002478_04580
• CAZy Family: GH2
• CAZyme Description: Exo-beta-D-glucosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
855	MGYG000002478_1|CGC83	98573.47	5.6438

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002478	5444912	Isolate	Finland	Europe

• Gene Location: Start: 5391431; End: 5393998 Strand: -

Full Sequence      

MKKTLKVLMMLGCFPMMLSAKEYKKSESLSLDKGWEFAQVGRNEWLPATVPGTVHQDLIS
HNKLPNPFYGMNEQKVQWVENEDWVYKTTFNVTDEQLSRDAALLILEGLDTYADIYLNGS
LLERTDNMFVGYTLPVKEVLRKGENHLQILFHSPVKQTLPQWETNGFDYPADNDHSDKRV
SIYSRKAPYSYGWDWGIRLVTSGIWRPVTLTFYDVARIDDYYVRQASVTKDLAKVENLLT
VNSVSATPQKAEVTVAYSYKEGEKVTEQKEVTLQPGTNHILLPIEIRQPHLWMPNGWGEP
ALYDFEAVIKVDGKVIASQKERIGLRTIKVVKEKDDQGESFYFVVNGEPMFAKGANFIPD
DALLPNITEERYRQLFKDVKDANMNMIRVWGGGIYEDDAFYQAADENGILVWQDFIFACT
TYPSDPVFMKRVEAEAEYNIKRLRNHASLAMWCGNNEIYEGMRYWGWDKKYTDPGIMEGM
KQGYDKLFRELLPRKVAELDPDRFYMHGSPYEANWGCPESWKIADSHNWGIWYGQKPFES
LDTEIPRFMSEFGFQAFPEMKTIATFASPEDYALESEVMNAHQKATIGNFLIKKTMGLYY
KVPEDFDQLVYMGLVLQGVGVRQGLEAHRRNRPYCMGTLYWQLNDSWPVVSWSSIDYYGN
WKALHYQAKRAFAPVLVDAVKEGEDLNIYVMSDKLEADKEVTLLLRVMDFNGKVLTKKSI
KGEVPANASTLYYKEPYAGITTSPQNTFLLMTLKNKKGEVLSEEIYYFNHAKDQELPKTE
IRYKVKPMDGKYEVTLSARQLARDVFIEIPVQGAKFTDNFFDLLPGQTKKVIITSDKLKK
NEKVDITIKQLSDTN

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	20	758	6.2e-111	0.7340425531914894

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.80e-118	17	835	1	786	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17786	Mannosidase_ig	2.06e-18	684	773	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
pfam17753	Ig_mannosidase	9.27e-17	778	853	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam00703	Glyco_hydro_2	2.42e-15	216	326	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	3.63e-12	80	457	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALA75885.1	0.0	1	855	1	855
QJR54343.1	0.0	1	855	1	855
QJR60858.1	0.0	1	855	1	855
AII70175.1	0.0	1	855	1	855
QJR73623.1	0.0	1	855	1	855

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	0.0	26	854	6	837	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	0.0	26	854	8	839	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	0.0	26	854	6	837	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	0.0	26	854	8	839	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	0.0	26	854	6	837	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A1CGA8	2.97e-130	31	837	9	832	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q0CCA0	1.16e-128	30	837	8	830	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2
Q4WAH4	2.39e-128	25	833	3	828	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A2QYN2	6.42e-128	30	844	8	837	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
B0YBU9	9.23e-128	25	833	3	828	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001118	0.743601	0.254171	0.000414	0.000374	0.000294

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002478_04580.