CAZyme Information: MGYG000002483_00874

Basic Information

• Species: Clostridium_Q saccharolyticum_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Clostridium_Q; Clostridium_Q saccharolyticum_A
• CAZyme ID: MGYG000002483_00874
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
870	MGYG000002483_12|CGC2	97329.43	4.5899

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002483	3781880	Isolate	not provided	not provided

• Gene Location: Start: 231775; End: 234387 Strand: -

Full Sequence      

MDRERVEKNSRGENASGASEPVNAQKKPETGRQEEIIRELQEEEAVQRELCDSMRRILGE
EQDGQEKEESLQPEKPDLRKQEEEGTEEEKTSAGGQGEKGPGTGSPDMEKPAPMRPEKKT
GKGRQGRKRKKHRLLRRFLSVFFLLLFIALAGGGIFAWKFYTVCRDEVAGLVENSTESDF
LPSQASYVYDSAGEVMAKLAQDVDSQYLSYEEIPKAAVNAFVAVEDRTFWENCGVDFKGI
ARVGIHFILTGGEEVHGASTITQQLARNQYLTRDVSIRRKVKEIIIAMDLTEKYTKEQIM
EFYINDINFANTYYGLQAAARGYFGKDADELSLSQTAYLCAIPNSPSYYNPYRHPENALK
RRDKILGDMLEMGYITQEEHDAAVSEEISVSRTSYEMKNYETTYAIDCAVRWLMREDGFT
FEYGFTNYDDYRAYQEAYEESYDRAKDELYTGGYRIYTSLDPAKQSILQSAVDQGLSFDA
ETAENGIYALQGAAVAVNNDTGKVEAIVGGRSQETDTYTLNRAFQSFRQPGSSIKPLIVY
APALEDGYASFSRVKDISVDAAKKPGADVFSLPGNWITMRQAVERSRNGAAWWLFNEVTP
EEGLSYLTKMRFDNIVPDDYNMAASLGGFTYGVTAEEMAGAFCALENGGVYREPDCILRM
ENLEGDDIYTEREPKEVYSRQTAAVMVDMMKGVITRGTARGMGWNSSIEAAGKTGTTNNS
KDGWFCGVTPYYTVTVWVGYDTPRTLSSLYGSTYPAAIWKQAMEGLTAGLPEASFSAPEA
EKENIHKEGRGTYLEGYDDAYVLSPGYTVGDYRKDHALADEAQKYIDQMTGASPEEQERL
RKKAESAINRIYGQTLKGQMLQVLEGAGKS

Enzyme Prediction     

No EC number prediction in MGYG000002483_00874.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	193	369	4e-62	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	2.03e-172	206	764	3	526	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	6.03e-161	127	783	4	613	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	5.19e-139	179	779	42	731	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	6.73e-82	211	767	153	683	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	1.58e-71	193	370	2	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBK77227.1	0.0	1	563	1	563
BAK46854.1	8.73e-240	165	796	99	733
CBK80882.1	3.99e-232	109	856	35	789
BCK01696.1	2.24e-193	128	789	56	720
BBF44496.1	1.97e-184	165	783	49	665

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4OON_A	4.86e-71	188	778	23	734	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	5.82e-67	188	773	23	722	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
7U4H_A	1.20e-62	174	785	9	776	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3DWK_A	5.51e-57	188	768	10	590	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
3ZG8_B	4.02e-55	289	783	2	477	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P38050	2.11e-93	177	783	40	612	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
P40750	3.63e-88	171	764	41	621	Penicillin-binding protein 4 OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpD PE=1 SV=2
O66874	1.90e-82	182	785	41	683	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q8XJ01	1.48e-74	162	752	44	652	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
Q0TNZ8	8.16e-73	162	752	44	652	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000079	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
134	156