CAZyme Information: MGYG000002483_00922

Basic Information

• Species: Clostridium_Q saccharolyticum_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Clostridium_Q; Clostridium_Q saccharolyticum_A
• CAZyme ID: MGYG000002483_00922
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
642		72075.89	4.0804

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002483	3781880	Isolate	not provided	not provided

• Gene Location: Start: 292320; End: 294248 Strand: -

Full Sequence      

MKKKLPFAALTMAGILGASVWLQPLTAYAAAWERSGNVYEMPDGSPITGVFRRGIDVSHW
QQDVDWNQVAADDVDFVMLGTRYKGEVDPNFRKNADAAYAAGVELGAYIYSYATSVEMAE
QEADFILDLVKDYPISYPIAFDAEDAGTLGTLPPEQVSQIINAFCKKIEDAGYHSMVYAN
EYWLNNKIDRDSLDYDVWVARYNVMYTYDSPAIWQATNTGSVNGIAGNVDIDFQFKDYSS
VIPADTWREIGQNRYYYQNYVMQKDAWIHDGNGWYYMNSSGNPAKGWQEFHDGTYYLEEP
DGRMAEGWRELDQNYYYFNSSGRMTTGWQDVGGTWYYMDSEGHMQTGWQDIDGKRYYLNS
SGAMLSGWQDIDGARYYLNASGAMQTGWQQLDGTWYYLGEDGKRRTDWQQVGESWYYLDG
EGRMLTGWQDIGGSRYYLNGSGAMLTGWQDIEGARYYLNSSGAMLSGWQDIDGSRYYLGT
DGRMAAGWQDIDGGRYYLGTDGRMATGWQDIDGGRYYLGTDGRMVTGWQQLDGTWYYLNS
DGRMATGWKKSGETWYYLNSEGQMQTGWIEVDGALYYLNESGAMAVNTILDRNGVKYQAD
GSGACTEVPADSAAGDTAQSTEQAAPSDSGQQSGPAGPASDF

Enzyme Prediction     

No EC number prediction in MGYG000002483_00922.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	55	225	1.2e-43	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF033930	pneumo_PspA	5.28e-77	383	601	440	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
cd06414	GH25_LytC-like	3.36e-69	52	235	1	191	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
NF033838	PspC_subgroup_1	4.79e-68	405	595	485	676	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.
NF033838	PspC_subgroup_1	1.07e-56	283	464	484	665	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.
NF033930	pneumo_PspA	1.89e-55	243	444	441	642	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRV19588.1	2.63e-96	7	264	10	268
ADL06311.1	2.63e-96	7	264	10	268
SET96139.1	1.13e-95	7	264	10	268
CBK77005.1	3.34e-95	12	264	12	262
QQR03194.1	4.62e-92	26	264	74	311

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WW5_A	5.24e-11	55	231	272	464	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae at 1.6 A resolution [Streptococcus pneumoniae R6],2WWD_A 3D-structure of the modular autolysin LytC from Streptococcus pneumoniae in complex with pneummococcal peptidoglycan fragment [Streptococcus pneumoniae R6]
2WWC_A	1.21e-10	55	231	272	464	3D-structureof the modular autolysin LytC from Streptococcus pneumoniae in complex with synthetic peptidoglycan ligand [Streptococcus pneumoniae R6]
5A6S_A	5.14e-08	87	245	58	212	Crystalstructure of the CTP1L endolysin reveals how its activity is regulated by a secondary translation product [Clostridium phage phiCTP1]
1JFX_A	1.90e-07	53	230	6	198	Crystalstructure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution [Streptomyces coelicolor]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P34020	4.57e-09	53	232	2	178	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1
Q8X7H0	3.05e-08	54	232	69	252	Uncharacterized protein YegX OS=Escherichia coli O157:H7 OX=83334 GN=yegX PE=3 SV=2
P76421	4.09e-08	54	232	69	252	Uncharacterized protein YegX OS=Escherichia coli (strain K12) OX=83333 GN=yegX PE=3 SV=2
Q8FFY2	5.48e-08	54	232	69	252	Uncharacterized protein YegX OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=yegX PE=3 SV=2
P25310	2.13e-06	53	230	83	275	Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000522	0.998515	0.000222	0.000289	0.000228	0.000195

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002483_00922.