CAZyme Information: MGYG000002484_00105

Basic Information

• Species: Cronobacter malonaticus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Cronobacter; Cronobacter malonaticus
• CAZyme ID: MGYG000002484_00105
• CAZy Family: GH23
• CAZyme Description: Soluble lytic murein transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
644	MGYG000002484_4|CGC1	72969.65	8.1291

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002484	4470595	Isolate	Czech Republic	Europe

• Gene Location: Start: 2416; End: 4350 Strand: +

Full Sequence      

MGTSRQVVWRWLAAGVCLLTLGQAARADSLDEQRSRYAQIKQAWDNRQMDVVEQLMPTLQ
TYPLYPYLQYRQLTDDLMNEPTIAVQQFIQANPTLPPARTLTSRFVNELARREDWRGLLA
FSPQPPGSTEAQCNYYYAKWNTGDAQGAWQGAKDLWQTGKNLPAACERLFQAWRTSGAQD
PLAYLERIRLAMKEGNTSLVSSLASQLPPDYQTISSALVSLANDPNSVMTFVRSVGATDF
TRQMAAVAFASVARQDVENARLMIPSLIQAQQLNDAQAQELRDIVAWRLMGNDVTAEQAR
WRDDAIMRSESTSLIERRVRMALGIGDRSGLNTWLARLPMEAKEKDEWRYWQADLLMERG
RDDEAKTILHSLMQQRGFYPMAAAQRLGEDYPLRVDKAPAVNAALFEGPEMARVRELMYW
GLDNTARSEWANLVTSRSKQEQAALARYAFDRNWWDLSVQATIAGKLWDQLEERFPLAYK
DLFARYVSDKAVPQSYAMAIARQESAWNPKVRSPVGASGLMQLMPGTATHTVKMFNLPGY
SNSAQLLDPETNIQIGTSYLQYVYGMFDNNRIFASAAYNAGPGRVRTWLGNSAGRIDAVA
FVESIPFSETRGYVKNVLAYDAYYRYFMGGKPVLMSDAEWSRRY

Enzyme Prediction     

No EC number prediction in MGYG000002484_00105.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	496	621	4.3e-30	0.762962962962963

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11619	PRK11619	0.0	1	644	1	644	lytic murein transglycosylase; Provisional
cd13401	Slt70-like	4.78e-69	474	626	1	151	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
COG0741	MltE	4.55e-50	346	632	1	291	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
cd16896	LT_Slt70-like	4.01e-43	476	620	1	143	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	2.49e-37	490	599	8	113	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AHB71863.1	0.0	1	644	1	644
ALX79951.1	0.0	1	644	1	644
QXL97892.1	0.0	1	644	1	644
AXW93207.1	0.0	1	644	1	644
QWR91992.1	0.0	1	644	1	644

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1SLY_A	0.0	28	644	1	618	ComplexOf The 70-Kda Soluble Lytic Transglycosylase With Bulgecin A [Escherichia coli]
1QSA_A	0.0	28	644	1	618	CrystalStructure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.65 Angstroms Resolution [Escherichia coli],1QTE_A Crystal Structure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.90 A Resolution In Complex With A 1,6- Anhydromurotripeptide [Escherichia coli]
5OHU_A	3.38e-84	24	644	21	638	TheX-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa [Pseudomonas aeruginosa]
6FC4_A	3.73e-82	32	644	1	610	ChainA, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa]
6FBT_A	6.62e-82	33	644	1	609	ChainA, Lytic murein transglycosylase [Pseudomonas aeruginosa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0AGC3	0.0	1	644	1	645	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P39434	0.0	1	644	1	645	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P0AGC4	0.0	1	644	1	645	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
P44888	8.73e-72	272	644	227	593	Putative soluble lytic murein transglycosylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=slt PE=3 SV=1
P39433	2.89e-17	603	644	1	42	Soluble lytic murein transglycosylase (Fragment) OS=Enterobacter cloacae OX=550 GN=slt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000193	0.999215	0.000149	0.000152	0.000135	0.000127

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002484_00105.