CAZyme Information: MGYG000002484_01141

Basic Information

• Species: Cronobacter malonaticus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Cronobacter; Cronobacter malonaticus
• CAZyme ID: MGYG000002484_01141
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
789	MGYG000002484_39|CGC2	85634.25	5.4763

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002484	4470595	Isolate	Czech Republic	Europe

• Gene Location: Start: 91423; End: 93792 Strand: +

Full Sequence      

MTIYKDPTRPVAERVADLLARMTPEEKFAQMHAYWLVLSPDGDHRERTDLSDEFSGATQQ
AALAERLKRGAGQITRPLGTHIVAPREGVRAANRLQRMLVEETRLGIPAMFHEECLVGLL
CKDATLFPSSLNYGSTWDPALVEQAAQAIGQEARAVGCHQGLAPVLDVSRDVRWGRTEET
FGEDPWLVGLMATHYVKGLQGPKRDLLATLKHYVGHSFSEGARNHAPVHLGFCELSDTFL
LPFEMAVKLAHAGSVMPAYHDIDNVPTHADDFLLTKVLREQWGFDGIIVADYGGVSLLHQ
HHGVAQDAAHSAALAFNAGLDIELPKDDCARHLAQALERGLITMEKVDEIVARVLAEKFR
LGLFEHPYADENDIVLQSDDTRRIAREVAARSLTLLENNGVLPLKGTPRVAVVGPTADDP
LALLSGYSFPVHLIISDMLEQTSQVTTPLAALREQPGIALTGYAKGCHIIEKRMAGAPVF
PGDSGEKPMQQSPVSDDTSLIPEAVRLATESDVVLAFVGDLSGLFQSGTVGEGSDTDSLQ
LPGVQQQLLEALVEAGKPVVVVMTGGRPYHLGGLESCVAAWVMAWAPGQEGGHAIADLLT
GKHEPQGRLVVSVPKSAGAMPYYYNHKLKSGGTPYAFHFGSRYPFGYGKTWTTFRYGELE
IAQARVPMDGDVEASVTVTNSGTQPGSEVVQLYVRDKVASLVRPVQELKAFGRVSLAAGE
SACVTFRIPVDMLNFTSRDGVRIVEPGEFEIRVGANSGDIRSRCTVTVEGETHTLGNSWR
MVSECNIEQ

Enzyme Prediction     

EC	3.2.1.37

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	101	325	1.9e-65	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.73e-157	15	762	38	759	beta-glucosidase BglX.
COG1472	BglX	5.25e-87	22	420	1	367	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	4.58e-76	50	355	10	314	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	8.66e-68	104	754	78	769	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	3.72e-51	393	648	1	213	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALX78903.1	0.0	1	789	1	789
AHB70688.1	0.0	11	789	1	779
AXX01883.1	0.0	1	789	1	789
ABU77349.1	0.0	1	789	1	789
QGG06798.1	0.0	1	789	1	789

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5XXL_A	2.21e-130	18	769	17	754	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	1.21e-129	18	769	17	754	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]
5Z87_A	1.48e-126	4	761	36	778	ChainA, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5Z9S_A	2.41e-123	4	757	17	775	Functionaland Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum],5Z9S_B Functional and Structural Characterization of a beta-Glucosidase Involved in Saponin Metabolism from Intestinal Bacteria [Bifidobacterium longum]
5YOT_A	1.63e-122	4	769	7	757	Isoprimeverose-producingenzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33363	3.04e-112	15	761	38	758	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	8.81e-111	15	761	38	758	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
T2KMH0	1.25e-109	104	757	62	710	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
T2KMH9	7.83e-109	4	761	29	748	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
A7LXU3	1.60e-76	7	760	30	768	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002484_01141.