CAZyme Information: MGYG000002490_03177

Basic Information

• Species: Paenibacillus_B thiaminolyticus
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_B; Paenibacillus_B thiaminolyticus
• CAZyme ID: MGYG000002490_03177
• CAZy Family: GT2
• CAZyme Description: D-alanine--poly(phosphoribitol) ligase subunit 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
3074	MGYG000002490_29|CGC3	340069.34	5.5202

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002490	6537383	Isolate	Japan	Asia

• Gene Location: Start: 117873; End: 127097 Strand: +

Full Sequence      

MQEPQKLDKSNVEDILALTPMQSGMLLQYLKDPDTTQYVELIELGISGDIDTSLFEQSWS
CVVQANEALRTLFRWEEVNQPVQIVLKQHRPVIRYENYSELDEEARARQIAARIAADRQE
KFKLDQVPFRLTLCKQTGRNHTLLISFHHILLDGWSTGILLKEWLGAYRMLAQGMKPELR
RKPPFKQFVALLQQQDKKKQASYWSRYLHELEAKPIPVSRGGASPEKASIAVHTEKRSGT
YVADLHRFARTAQVTQAALFYTAWGILLQHYTNSDDVLFGVTVSGRNTSISGMEDMAGLF
INTLPLRLKAGPEHGLPDLLADVNRSIQAHEEHAGTSLTDIKGYGGMRTEGVPFDSLLVV
ENYPLDKAVTSAEGPLAIASHHIEEETEFDLTVRITAAEELEMTFAYNEQAHRSDTVAKL
ACHYLYIIEQIIQTSTQVTAYHETRLQDIGILTAEEREQIRHFNLTTASYPREKTIHELF
EEQVRLAPEGTAVTDEQRSCTYRELNEQANRIARALRADGVERGQVVGLMANRSIDMIAG
IFGILKAGGVYLPLDSAHPAERIAYMLQDGKVGTVLASAGLEPLVPEQTKVWVLNEALLG
CGEATDMPSVNGPEHPAYVMYTSGSTGTPKGVAVGHRNVVRLVRNTNYVQVEAGDCMLQA
GAIGFDALTFEVFGALLNGGSLHIADKHTLLDSSRLDAFLRSRNISVALLTPALFNQLAQ
QRPQMFAGLRHLIVGGDVLSPKHIEAVRQACPGLAMWNAYGPTENTVISTCFRIDREYAE
HIPIGPPISNSTAYIVDRHDNLLPIGVPGELLVGGDGVALGYLNQPELTSEKFVPDPYCP
GGVMYRTGDMACWQEDGSLEYMGRIDQQVKIRGHRIEIGEIETMLLRHPSVQEAAVTALQ
RGGHTELCAYVVASGQAPVSELRDYLAATLPDYMIPSHVVPLEHIPLTPNGKLDRKALPA
PAERVRSARDAALPRTELERLIAEAWQAVLELESVSIHDKYFEVGGNSINLIQLQSKLQR
QLKREVPIVTLFQYPTVHELAAHLAGEADGTRGRASEQAAASREDEPHSQAAAGSARPGN
EGSRDIAVIGMAGRFPGARSIAEFWSNLQQGQESISFFTDDELAEYGFDRDLLQRPEFVK
AKGVLDEMDHFDPAFFGYTPDQAAIMDPQVRLLHECAWHALEDAGCDPERYGGSIGLFAG
VTNNFHWLSQLSERLHGNLSDMFEVDSLNDTYTVSTRISHKLNLRGPAISLQTACSTSLV
AVHVACQSILSGDCDLALAGGASIVLPHKSGYLYQEGMVKSPDGHCRSFDAQAKGTIGGD
GVGFVALKPLEAAKKDGDRIYAVIKGSAINNDGSRKVGYTAPSVEGQMDVIKRALAAAQV
PSDSITYVEAHGSGTPLGDPIEIEALTKAFQTDRTGFCRIGSVKTNIGHLDAAAGVAGLM
KTALALHHKRIPQSLHFETPNPNIDFANSPFVVNTALAEWRNDAGPLRAGVSSFGIGGTN
AHVILEEAPTREAGGDGRSSQLLALSARTPAALDAMTANLGAYLDERRELCLADVAYTLQ
SGRRHFKYRRILSCDTIGEAIEALTPVEGRNSFDERKVVTFAADDGGSPVVFMFSGQGSQ
YVNMGADLYRDEPVFRAEMDRCFRLYRDIAGRDMKEILYPSGDAEAAALQLNQTKHIQPV
MFMFEYALARLVMAWGVKPEAMIGYSFGEFTAACLADVMSLEDAMRLIILRGELMQQVPA
GAMLSVPLPEQEVRLRLDGSLSLAVVNGPSCIVSGTEEEIAAFEHRMKAEKIMCMRLPVA
TAGHSSLLHAVKEPFAAAMRQVRLRPPALPYVSTTTGTWITAEEAADPDYWIRHLTDTVR
FADGMALLVQEPRRLFVEIGPGHDLTLLARRHLGSGREQQALNLVRPAQRGGSDSALLVN
RIGRLWLHGQSIDWQAFHGEVRAIISLPSYPFARERYWPQASGGALAAPGTARSKGRPAS
GQMGKQTDMSDWFYVPTWKKSPTAASRKGDAAASWKEDTAGNWLVFADGAGLAADLVKHL
SERGAQVVTVRAGERFEQEGTMQYTVHPRNERHYAKLFSELQADGRLPNRMLHAWGVSAP
SRSDQQWIRTAQDLGFYSVLYTAKALKQQQAREEMRMWVVTDSMHAIAGEPVLYPEKATV
LGPCRVIPQELPHIQCRSIDIVLPEAGSWQRDRLVGRLLDEFGAAGTELAVAYRDNARWV
LDYEPFTISEPEAQTQGPRQGGVYLITGGLGYIGHTLGGYLARSVQAKLALTSRSLFPPR
AEWDTWLSVHGKEDAVSVQIGKLRQLEAQGAEVLVLSADAADQGQMETVIRQAEAAFGPL
NGVIHAAGMTGDAAFRMLEETDELLCEQHFRAKMYGLLVLEQVLAGKELDFCLLASSLSP
MLGGLGFSAYAAANHFMDAFVHDRNRRQRIPWTSVNWDGWQLEADQTIRGQAGASISELL
IAPAEGVELLRRLLSVRDMSQIVISTGDLGARIDKWVRREGQQEEPEAFRHGEGSYYSRP
ALSGEYVAPATETERKLCGMWEQFFRIDRIGVQDDFFELGGDSLKAITVVSAIHKELSVE
IGLPVFFNMPTIQQLSAYIDGAERSAYHDIESAEAKDYYELSSAQKRLYLIQQMEQGHTG
YNEIVAGVLEGRLDRTRLEAALRRLVERHESLRTSFELVNGKPMQKINEAVAIELEYEDY
SAAVTPLDSLDGEEKMDDRIRAAVATFVRSFDLTRAPLMRAGLIKLGEERHVLMVDLHHI
VSDGLSQDIFVNDFLALYAGRELPELPLQYKDFSEWQNRMMDTDEMKRQGAFWLERLKDA
PRLSLRTDYPRPDTRSFAGSLVPFELDAERTQGLKSLCLKEDVTLFMALLAIFNLLLAKV
CGQDDIVVGTPVVGRKQQALQLIVGKFVNMLPLRNRLQEEMSFRNLLQAVRSTTLDAFAH
QDFQFEEMVQQAGRERELGRNPIFDVVFALQNMQNPEMSIPGLILKPFPFIHDASHFDLS
LIAEEDGDRLAFKFEYSTRLFQRDTIDRFAAYLQDIVFDVLAHPDKKIQEIKIAHHLAEP
EAVFLAGAQGEFGL

Enzyme Prediction     

No EC number prediction in MGYG000002490_03177.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd12117	A_NRPS_Srf_like	0.0	478	958	1	483	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
cd05930	A_NRPS	0.0	488	958	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK12316	PRK12316	0.0	7	1115	1548	2668	peptide synthase; Provisional
PRK12467	PRK12467	0.0	32	1053	66	1106	peptide synthase; Provisional
PRK12467	PRK12467	0.0	32	1051	1133	2169	peptide synthase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAY90071.1	0.0	163	3061	269	2678
BAY30132.1	0.0	245	3061	361	2689
BAZ00088.1	0.0	245	3053	361	2682
BAZ75991.1	0.0	245	3053	361	2682
AFY93865.1	0.0	449	1958	305	1861

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VSQ_A	1.26e-179	6	1050	3	1046	Structureof surfactin A synthetase C (SrfA-C), a nonribosomal peptide synthetase termination module [Bacillus subtilis]
4MZ0_A	9.81e-167	1086	1958	41	935	ChainA, CurL [Moorena producens 3L],4MZ0_B Chain B, CurL [Moorena producens 3L]
7S6C_A	1.64e-158	1083	2440	31	1372	ChainA, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6C_B Chain B, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6C_C Chain C, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6C_D Chain D, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6D_A Chain A, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6D_B Chain B, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct],7S6D_C Chain C, 6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion [synthetic construct]
7M7J_A	7.95e-155	1086	2599	35	1485	ChainA, EryAI [Saccharopolyspora erythraea],7M7J_B Chain B, EryAI [Saccharopolyspora erythraea]
7M7I_A	7.95e-155	1086	2599	35	1485	ChainA, EryAI [Saccharopolyspora erythraea],7M7I_B Chain B, EryAI [Saccharopolyspora erythraea]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O30409	2.39e-278	44	3067	3159	5644	Tyrocidine synthase 3 OS=Brevibacillus parabrevis OX=54914 GN=tycC PE=1 SV=1
P0C064	3.43e-271	13	3051	1059	3562	Gramicidin S synthase 2 OS=Brevibacillus brevis OX=1393 GN=grsB PE=1 SV=2
P0C063	1.68e-268	13	3051	1059	3563	Gramicidin S synthase 2 OS=Aneurinibacillus migulanus OX=47500 GN=grsB PE=3 SV=2
Q04747	1.00e-266	7	3051	1	2515	Surfactin synthase subunit 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAB PE=1 SV=3
O30408	1.22e-260	9	3053	6	2520	Tyrocidine synthase 2 OS=Brevibacillus parabrevis OX=54914 GN=tycB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002490_03177.