CAZyme Information: MGYG000002490_04137

Basic Information

• Species: Paenibacillus_B thiaminolyticus
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_B; Paenibacillus_B thiaminolyticus
• CAZyme ID: MGYG000002490_04137
• CAZy Family: GT2
• CAZyme Description: Gramicidin S synthase 2

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2388	MGYG000002490_36|CGC10	272018.11	4.8957

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002490	6537383	Isolate	Japan	Asia

• Gene Location: Start: 534448; End: 541614 Strand: -

Full Sequence      

MSMEEQVNYWLTELAGEIPLLQLPIDNSGNKNYSYAREIESIVLSESISERIKSLGLQEE
TNVFTLLLTAYQSYLYRYTGQNDIWVGVMTGCRSALFVNRAFVNGTMNFKQLLAEVKDKV
AKGQANQDVPYEVIAEKLASIHAGSTELFQTLFRYIEPGDEDAKGMLYNDSSLPLIRLHV
DIAEAADGLQISFAYNACLFAEQTIRRMIENFRCWIEQVTTHVDTPIDQLRLITKEQERE
LLDAGCRNDANMLPDTILTAFDGQVQRNPDAIALVFGEKKSTYRELDVRSNQLANYLRKT
GVTNETLVGICLERSIDMLVSILGVMKAGGAYVPMDPAYPIQRLHYIIEDAGLQVIITNE
AFLAAVPEGVQVVHLSDNDNTLWDESAEKPDIKVNGSNLAYVIYTSGSTGNPKGVMIEHH
SIMNFLQTLESRDELAKSDRLLQKTSVSFDASVWELFWWMLKGASLYILPNGDEKDPVLL
VKAVERYQVTHLDFVPSMLKVVLDYIENQGSSSKLSSLKYVTVGGEVLPSHVAQKFADLL
TTPYGTILYNAYGPTETTVDVARFKCDPDEKRDQIPIGKPNSNTQLFVLSEHLQVQPVGV
IGELFVGGSGVARGYLNRPQLTEERFISNPYGPEPHSRLYRTGDLVRYLADGNLEYICRT
DNQVKVRGYRIELEEIQVILSSHPNIDQSIIVAKKDAQENNKLVAYVIGTGSITDWREYL
KNRLPEYMVPAYFVKMDAFPLTPSGKIDVKSLPEVGNSRPHITTDYIKPETDLEHKLADV
WKDLFGYDEIGVEDNFFELGGHSLLGTQVLARIRELVKKELPLSALFTYPTIRSMVPVLH
AADDFIPMDTFPRISRASRDRELPLSYSQERVWFLEQLSSNNLAYIFQATMNVRGRLDIP
VLEKCFTEIVRRHEIFRTVFLDKHGKPYQFIYEPFDVQLPVIDVSHLPESDRDQEAQRLI
QLEITKPIDITQLPLARWIVFTISEFESVILLIEHHLVHDGWSFRKFLKELFTLYSAYVE
NKPSPLPELPIQFADYCVWQNEMFQRGKESKQLTYWTHKLSGAKGVLELSTDRPRPVNQT
FNGNSIIRLIPEELYLQLRDYCVRTNTTLFMVMMAAFQTLLHRYSNQEDIIVGSGIANRR
WQETEELIGMFVNNIVIRTQFTENITFQDVLHKVRTSSLEAYENQDIPFDQVVDALRLER
DQSRNPLFQVMFSFHDTKITNLPVHNLDVQLLEGFSNGSAKFDINVVVINHSELSSSLLS
GDEYDSISMDWEYNTDLFDETTIRRMIEHYIELLHSILENSNQTVHSLPMLPDAEQNQLL
REWNNTNTKLEDTRTIHQIFEEQVARTPERIAVVCENEQWTYRQINNLANLLASKLRNLG
VKPDTVVGLMVERSPDMIIGILAILKAGGAYLPIDTAAPTERLAYILEDSDTKAIVMQEK
FKTSTDFQRPVLVLGENMPDQHVDCDNLEPAANFKNLAYVMYTSGSTGTPKGVMIEHDSV
VDRIHWMVKRYPMDQNDTILQKTSFCFDVSVTELLIWFFSGSKLCFLAPDAEKDPELIVN
TIAEQKVTFIQFVPSMLSIFLDHLENSNTSALIKSLKRVFTIGEALTVEQVQRFERLIKQ
RNDTTLHNLYGPTEATIEVTSYDCQAESKVIPIGKPIDNVKAYIFNKERNLQPVGVIGEL
FIGGTGLARGYVGKPELTAERFVPNPCGSDPEEKVYQTGDLARWMPDGNIEFIGRMDTQV
KIRGYRIELGEIEAVMRKLQDAGEAVVMAHEYEPGDTRLVAYYNGTAEASQVKLHLQKQL
PHYMIPSYFVQMEALPLTSSGKLDRKALLAPDMHAVSHHDTAPRNSTEELLALIWNEILR
VEPIGVFDSFFDLGGHSLLAAQVVSRVREVFGQHLPLRAIFDCPTIESLAKRLTESRQGG
NIAHLPALHPAERTGAFPLSFAQKRLWFFDQLEPDSYFYNIPYVWRLSGSWNAAGLEKGF
NQLIERHEMLRTVFAKRNGAPVQIVQPYQPRSLPVIDVSALSAEARESQINHHIQRNAEL
VFDLSQGPLIEAELIKKDECEYILLCTVHHIVSDGWSEAILLDEWLAFYEEAVSGTPADL
RPLAIQYSDFAVWNRQWLSDAMMTEQLEYWKNELAGELPVLQLPIDRPRPAIQSYAGDMQ
QLELAPSLLEKLKMFSRQEGTTLFMTLLAAYQGFLSRYTGQTDILVGSPVANRNITEIEG
LIGFFVNTLVYRVNVEDSPSFRQLIAQVKEKALRGQENQDVPFEKIVETLQPTRNPSYAP
IFQTIFTMHTHLRKIKEWPNRKIEPVKTCIKVAKYDLSVTLEVNNERTLDISFGYNKDLF
DHSTIERMIGHFANWLEQVVTYPDESIDNLRLITEDEEKLLLELWSSN

Enzyme Prediction     

No EC number prediction in MGYG000002490_04137.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12467	PRK12467	0.0	4	2263	237	2501	peptide synthase; Provisional
PRK12316	PRK12316	0.0	51	2206	1779	3900	peptide synthase; Provisional
cd17655	A_NRPS_Bac	0.0	1338	1832	1	490	bacitracin synthetase and related proteins. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
cd05930	A_NRPS	0.0	269	752	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK12316	PRK12316	0.0	864	2385	1558	3046	peptide synthase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	0.0	205	1917	961	2666
BAY90071.1	7.84e-264	1	2144	309	3529
BAZ00088.1	8.11e-261	1	2144	310	3538
BAZ75991.1	8.11e-261	1	2144	310	3538
BAY30132.1	4.63e-259	1	2144	310	3540

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6MFZ_A	0.0	160	1917	94	1801	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	4.29e-309	160	1832	94	1716	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
5U89_A	4.36e-222	1313	2382	4	1071	Crystalstructure of a cross-module fragment from the dimodular NRPS DhbF [Geobacillus sp. Y4.1MC1]
6P1J_A	1.56e-211	864	1828	7	964	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae],6P1J_B The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae]
6OYF_A	1.72e-183	864	1744	4	873	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module [Eleftheria terrae],6OZV_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with AMP [Eleftheria terrae],6P4U_A The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo1 serine module in complex with Mg and AMP [Eleftheria terrae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39847	0.0	216	2139	424	2292	Plipastatin synthase subunit C OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsC PE=1 SV=2
P94459	0.0	4	2382	194	2519	Plipastatin synthase subunit D OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsD PE=1 SV=2
P45745	0.0	4	1938	193	2129	Dimodular nonribosomal peptide synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbF PE=1 SV=4
Q04747	0.0	4	2385	193	2530	Surfactin synthase subunit 2 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAB PE=1 SV=3
P39846	0.0	4	2362	196	2526	Plipastatin synthase subunit B OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999632	0.000356	0.000013	0.000002	0.000001	0.000011

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002490_04137.