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CAZyme Information: MGYG000002511_00198

You are here: Home > Sequence: MGYG000002511_00198

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Klebsiella variicola
Lineage Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella variicola
CAZyme ID MGYG000002511_00198
CAZy Family GT20
CAZyme Description Trehalose-6-phosphate synthase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
474 MGYG000002511_1|CGC3 53801.13 6.4488
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000002511 5624300 Isolate Australia Oceania
Gene Location Start: 218436;  End: 219860  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 2.4.1.15

CAZyme Signature Domains help

Family Start End Evalue family coverage
GT20 2 452 8.6e-169 0.9705263157894737

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK10117 PRK10117 0.0 1 474 1 474
trehalose-6-phosphate synthase; Provisional
COG0380 OtsA 0.0 1 460 14 486
Trehalose-6-phosphate synthase [Carbohydrate transport and metabolism].
TIGR02400 trehalose_OtsA 0.0 3 452 1 455
alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., ). [Cellular processes, Adaptations to atypical conditions]
cd03788 GT20_TPS 0.0 3 452 1 463
trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
pfam00982 Glyco_transf_20 0.0 2 452 1 469
Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QOV60291.1 0.0 1 474 1 474
QXN96760.1 0.0 1 474 1 474
AQL21923.1 0.0 1 474 1 474
QLS59504.1 0.0 1 474 1 474
QKK54231.1 0.0 1 474 1 474

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
2WTX_A 1.60e-295 1 474 1 474
Insightinto the mechanism of enzymatic glycosyltransfer with retention through the synthesis and analysis of bisubstrate glycomimetics of trehalose-6-phosphate synthase [Escherichia coli K-12],2WTX_B Insight into the mechanism of enzymatic glycosyltransfer with retention through the synthesis and analysis of bisubstrate glycomimetics of trehalose-6-phosphate synthase [Escherichia coli K-12],2WTX_C Insight into the mechanism of enzymatic glycosyltransfer with retention through the synthesis and analysis of bisubstrate glycomimetics of trehalose-6-phosphate synthase [Escherichia coli K-12],2WTX_D Insight into the mechanism of enzymatic glycosyltransfer with retention through the synthesis and analysis of bisubstrate glycomimetics of trehalose-6-phosphate synthase [Escherichia coli K-12]
1UQT_A 2.16e-295 1 474 1 474
Trehalose-6-phosphatefrom E. coli bound with UDP-2-fluoro glucose. [Escherichia coli],1UQT_B Trehalose-6-phosphate from E. coli bound with UDP-2-fluoro glucose. [Escherichia coli],1UQU_A Trehalose-6-phosphate from E. coli bound with UDP-glucose. [Escherichia coli],1UQU_B Trehalose-6-phosphate from E. coli bound with UDP-glucose. [Escherichia coli]
6JAK_A 2.53e-285 2 456 1 455
OtsAapo structure [Escherichia coli],6JAK_B OtsA apo structure [Escherichia coli],6JAK_C OtsA apo structure [Escherichia coli],6JAK_D OtsA apo structure [Escherichia coli]
1GZ5_A 2.06e-282 2 457 1 456
Trehalose-6-phosphatesynthase. OtsA [Escherichia coli],1GZ5_B Trehalose-6-phosphate synthase. OtsA [Escherichia coli],1GZ5_C Trehalose-6-phosphate synthase. OtsA [Escherichia coli],1GZ5_D Trehalose-6-phosphate synthase. OtsA [Escherichia coli]
5UOF_A 3.37e-178 1 466 9 476
Crystalstructure of alpha,alpha-trehalose 6-phosphate sythase from Burkholderia multivorans [Burkholderia multivorans ATCC 17616],5UOF_B Crystal structure of alpha,alpha-trehalose 6-phosphate sythase from Burkholderia multivorans [Burkholderia multivorans ATCC 17616]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
A6TB47 0.0 1 474 1 474
Trehalose-6-phosphate synthase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) OX=272620 GN=otsA PE=3 SV=1
A7MEE9 3.16e-304 1 474 1 474
Trehalose-6-phosphate synthase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=otsA PE=3 SV=1
A4WBR1 2.37e-299 1 474 1 474
Trehalose-6-phosphate synthase OS=Enterobacter sp. (strain 638) OX=399742 GN=otsA PE=3 SV=1
B1J0J4 8.77e-295 1 474 1 474
Trehalose-6-phosphate synthase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / NBRC 3972 / NCIMB 8545 / WDCM 00012 / Crooks) OX=481805 GN=otsA PE=3 SV=1
P31677 8.77e-295 1 474 1 474
Trehalose-6-phosphate synthase OS=Escherichia coli (strain K12) OX=83333 GN=otsA PE=1 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.977368 0.022275 0.000114 0.000059 0.000040 0.000164

TMHMM  Annotations      download full data without filtering help

start end
21 43