CAZyme Information: MGYG000002516_01705

Basic Information

• Species: Proteus vulgaris
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Proteus; Proteus vulgaris
• CAZyme ID: MGYG000002516_01705
• CAZy Family: GT2
• CAZyme Description: Cellulose synthase catalytic subunit [UDP-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
856	MGYG000002516_1|CGC15	99287.48	9.4602

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002516	3639158	Isolate	Denmark	Europe

• Gene Location: Start: 1804977; End: 1807547 Strand: +

Full Sequence      

MPRKKKEKKVPQHILIRYYKFYRHNGASRSATIVSELFMGIMWIFLRMESPFWRKIARKQ
RLLFPHINPRCPKVFDPLRYLLQSIWLICHYLVQLLVKKPTQFISYVRNLYKKLFVHVSP
IAIELIEHKKKNTSIWQYLLYSAIAVLAVFFILLSITQPFDLTFQFVFVIVLWLVALALR
DIHGRFASIAIIMLALTIACRYIWWRYQSTLYWVDNISLFFGLLLLLAETYAWCVLFLSF
MQCIWPLHRKPISMPEDITKWQSVDIFIPTYNEDLQIVKPTIYASLNLDWPKDKLTIYLL
DDGDRPEFKAFAQEVGIRYIAREKHNFAKAGNINHALSLASGEFVAIFDCDHIPTRSFLQ
FTMGWFLQDEKMALVQTPHHFFSPDPFERNLGNFRETPNEGTLFYGLVQDGNDTWNATFF
CGSCAVLRRTALDEIGGIAVETVTEDAHTSLRLHRHGWRSAYIRIPQAAGLATESLSAHI
GQRIRWAKGMVQIFRLDNPLFGKGLSIPQRLCYLNAMLHFFSGIPRMIFLLAPLTFLIFH
AYIIYAPAIAIALYVVPYLIHISLASSKLQGAYRHSFWGEIYETILAWYTTRPVLSTLFS
PYKGKFNVTEKGGVMEEGFVDWNINRPYLLFFNLNLLGILFAFWRIATGDPDEAWATVMC
LLWVCYNLILLGVAIAVSVETKQQRRFPRVRIKAPAMLLLNNGALYRCYLYDFSDNSCAI
LLPDNVILSLSQNENITLLLTQNQREHAFKANISRIEGQIIGLQLLEMTTQKAIDFTACT
FERADTWADWQNELPADKPLSSLKNIIFISMQGYNTLLKRAPYFIYATFRLLGLFLLWLS
SLLPQRVNVSQIFHQS

Enzyme Prediction     

EC	2.4.1.12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	265	436	2.1e-37	0.9941176470588236

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR03030	CelA	0.0	143	822	12	710	cellulose synthase catalytic subunit (UDP-forming). Cellulose synthase catalyzes the beta-1,4 polymerization of glucose residues in the formation of cellulose. In bacteria, the substrate is UDP-glucose. The synthase consists of two subunits (or domains in the frequent cases where it is encoded as a single polypeptide), the catalytic domain modelled here and the regulatory domain (pfam03170). The regulatory domain binds the allosteric activator cyclic di-GMP. The protein is membrane-associated and probably assembles into multimers such that the individual cellulose strands can self-assemble into multi-strand fibrils.
PRK11498	bcsA	0.0	15	856	4	852	cellulose synthase catalytic subunit; Provisional
cd06421	CESA_CelA_like	2.52e-123	263	494	2	234	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
COG1215	BcsA	4.39e-53	228	622	19	389	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06437	CESA_CaSu_A2	2.75e-33	263	489	2	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VTP73878.1	0.0	1	856	1	856
AYY81656.1	0.0	4	856	9	861
QPN88135.1	0.0	3	856	8	861
QIF94970.1	0.0	3	856	8	861
ATM99787.1	0.0	4	856	9	861

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LBY_A	0.0	15	851	17	860	ChainA, Cellulose synthase catalytic subunit [UDP-forming] [Escherichia coli K-12]
5EJ1_A	4.53e-103	201	696	67	579	ChainA, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
4HG6_A	2.57e-102	201	696	79	591	ChainA, Cellulose Synthase Subunit A [Cereibacter sphaeroides]
4P00_A	2.63e-102	201	696	80	592	ChainA, Cellulose Synthase A subunit [Cereibacter sphaeroides 2.4.1],4P02_A Chain A, Cellulose Synthase subunit A [Cereibacter sphaeroides 2.4.1],5EIY_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1],5EJZ_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
6WLB_A	3.74e-07	434	544	678	794	Structureof homotrimeric poplar cellulose synthase isoform 8 [Populus tremula x Populus tremuloides],6WLB_B Structure of homotrimeric poplar cellulose synthase isoform 8 [Populus tremula x Populus tremuloides],6WLB_C Structure of homotrimeric poplar cellulose synthase isoform 8 [Populus tremula x Populus tremuloides]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8Z291	0.0	19	846	21	855	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhi OX=90370 GN=bcsA PE=3 SV=1
Q93IN2	0.0	19	846	21	855	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bcsA PE=3 SV=1
P37653	0.0	15	851	17	860	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli (strain K12) OX=83333 GN=bcsA PE=1 SV=3
Q8X5L7	0.0	15	851	17	860	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli O157:H7 OX=83334 GN=bcsA PE=3 SV=2
P58931	2.61e-236	143	817	40	712	Cellulose synthase catalytic subunit [UDP-forming] OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=bcsA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999964	0.000035	0.000000	0.000000	0.000000	0.000001

TMHMM  Annotations     

start	end
27	46
80	97
138	157
162	179
186	205
218	240
517	539
543	565
628	647
657	679
821	843