CAZyme Information: MGYG000002517_00813

Basic Information

• Species: Roseburia hominis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Roseburia; Roseburia hominis
• CAZyme ID: MGYG000002517_00813
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
600	MGYG000002517_1|CGC10	68576.9	4.7461

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002517	3592125	Isolate	United Kingdom	Europe

• Gene Location: Start: 883661; End: 885463 Strand: +

Full Sequence      

MLYPRENRVRGVIDLGGVWNFKLGDEKEPGDSLVMGEDTELMAVPASYNDQKDDPKYRNH
YGWAYYERKITVPSYLKGQRLVLRFDAVTHAAKVYLNGRFLTAHKGGFLPFEVDVTDQLV
PGETAELVVAADNRVNHSTLPVGNEGSTAFFGSDNPGVPSVEAAKVWRKPQNLPNFDFFN
YAGINRPVRIYTTPAAYIEDITLVPDIDGDTGVVNYEIKTAGSVCEDEVRIRILDADGNE
VAAADGAGGTVRIPDARLWWPWPGEPYLYTASVTYGEDLYEETFGIRTVAVEGTQFLING
KPFYFKGFGKHEDAAFRGRGLDVCLDVKDINLIHWLNANSFRTSHYPYAEEMYQLCDREG
IVVIDETPAVGIGAGDKIDPYKTFHIREHHEQVLREMIARDKNHPCVVMWSMGNEPDTEH
FPESAYDYWHSLYELTHSLDPADRPVTFVCCQNNYERDLVTRTMDVVCVNRYYGWYNLSG
DLDAACYALNMELDFWEKQNKPVMITEYGADAVAGIHESVPEMFSEEFQMEYYRRQNVEF
DKRSFFIGEQVWNFADFATIQGCMRADGNKKGLFTRDRRPKMAAHYFKERWGQIPNFEYK

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	9	589	1.6e-109	0.6090425531914894

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	1	600	1	601	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.97e-117	1	591	1	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	7.59e-86	289	596	1	302	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	3.23e-38	66	509	113	514	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.42e-32	66	446	124	485	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEN95939.1	0.0	1	600	6	605
CUO31707.1	0.0	1	600	1	600
VCV23245.1	0.0	1	600	1	599
CBL08206.1	0.0	1	600	1	599
CBL12719.1	0.0	1	600	1	599

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U7I_A	2.47e-304	1	600	1	597	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]
6LD0_A	1.72e-245	1	600	3	669	ChainA, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1]
5Z1B_A	2.98e-244	1	600	25	691	Structureof Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_B Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_C Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_D Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679]
6D4O_A	3.75e-213	1	600	4	612	Eubacteriumeligens beta-glucuronidase bound to an amoxapine-glucuronide conjugate [Lachnospira eligens]
6BO6_A	1.36e-212	1	600	1	609	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4FAT7	7.38e-164	1	596	28	631	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
P05804	1.60e-161	1	591	1	588	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
O97524	1.86e-158	1	596	27	630	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
O18835	4.18e-157	1	596	27	630	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
P06760	1.71e-155	1	596	27	627	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002517_00813.