CAZyme Information: MGYG000002524_01323

Basic Information

• Species: Yersinia bercovieri
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Yersinia; Yersinia bercovieri
• CAZyme ID: MGYG000002524_01323
• CAZy Family: GT4
• CAZyme Description: Glutamate/aspartate import solute-binding protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
309	MGYG000002524_4|CGC2	33792.58	8.884

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002524	4273500	Isolate	Finland	Europe

• Gene Location: Start: 174763; End: 175692 Strand: -

Full Sequence      

MHMRKLALALLLAGMASGVAQAEEAAAQPAAKAEASVDTLKKIKDSGVIVVGHRESSVPF
SYYDNQQKVVGYAQDYSDLIVDAVKKKLNAPDLQVKLIPITSQNRIPLLQNGTFDFECGS
TTNNLERQQQASFSNTIFVVGTRLLTKKGSEVKDFKDLAGKTVVVTSGTTSEVLLNKLNE
KDNMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDQWDIVGTAQSQEA
YGCMLRKNDPAFKALLDETIATAQTSGVAEKSFDRWFKNPIPPKNLNLNFSLSDEMKALF
KAPNDKALN

Enzyme Prediction     

No EC number prediction in MGYG000002524_01323.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10797	PRK10797	0.0	1	309	1	302	glutamate and aspartate transporter subunit; Provisional
cd13688	PBP2_GltI_DEBP	1.53e-114	40	277	1	238	Substrate-binding domain of ABC aspartate-glutamate transporter; the type 2 periplasmic binding protein fold. This subfamily represents the periplasmic-binding protein component of ABC transporter specific for carboxylic amino acids, including GtlI from Escherichia coli. The aspartate-glutamate binding domain belongs to the type 2 periplasmic binding protein fold superfamily (PBP2), whose many members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
cd01000	PBP2_Cys_DEBP_like	1.22e-79	40	277	1	228	Substrate-binding domain of cysteine- and aspartate/glutamate-binding proteins; the type 2 periplasmic-binding protein fold. This family comprises of the periplasmic-binding protein component of ABC transporters specific for cysteine and carboxylic amino acids, as well as their closely related proteins. The cysteine and aspartate-glutamate binding domains belong to the type 2 periplasmic binding protein fold superfamily (PBP2), whose many members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
cd13689	PBP2_BsGlnH	1.44e-64	40	278	1	229	Substrate binding domain of ABC glutamine transporter from Bacillus subtilis; the type 2 periplasmic-bindig protein fold. This group includes periplasmic glutamine-binding domain GlnP from Bacillus subtilis and its related proteins. The GlnP domain belongs to the type 2 periplasmic binding protein fold superfamily (PBP2), whose many members are involved in chemotaxis and uptake of nutrients and other small molecules from the extracellular space as a primary receptor. The PBP2 proteins are typically comprised of two globular subdomains connected by a flexible hinge and bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap. After binding their specific ligand with high affinity, they can interact with a cognate membrane transport complex comprised of two integral membrane domains and two receptor cytoplasmically-located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis.
pfam00497	SBP_bac_3	4.78e-55	49	277	1	223	Bacterial extracellular solute-binding proteins, family 3.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QWJ92671.1	3.51e-168	1	309	1	297
QLQ94799.1	3.51e-168	1	309	1	297
QQE94206.1	3.51e-168	1	309	1	297
QXX81055.1	7.07e-168	39	309	27	297
BBV05439.1	1.00e-167	1	309	1	297

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VHA_A	4.22e-168	33	309	3	279	DEBP[Shigella flexneri],2VHA_B DEBP [Shigella flexneri]
2IA4_A	1.05e-161	33	309	3	279	Crystalstructure of Novel amino acid binding protein from Shigella flexneri [Shigella flexneri 2a str. 301],2IA4_B Crystal structure of Novel amino acid binding protein from Shigella flexneri [Shigella flexneri 2a str. 301]
5EYF_A	5.64e-28	35	279	3	238	CrystalStructure of Solute-binding Protein from Enterococcus faecium with Bound Glutamate [Enterococcus faecium DO],5EYF_B Crystal Structure of Solute-binding Protein from Enterococcus faecium with Bound Glutamate [Enterococcus faecium DO]
6SVF_A	3.00e-20	40	277	3	227	Crystalstructure of the P235GK mutant of ArgBP from T. maritima [Thermotoga maritima MSB8]
4PRS_A	7.09e-18	40	277	2	225	Structureof apo ArgBP from T. maritima [Thermotoga maritima MSB8],4PRS_B Structure of apo ArgBP from T. maritima [Thermotoga maritima MSB8],4PSH_A Structure of holo ArgBP from T. maritima [Thermotoga maritima MSB8],4PSH_B Structure of holo ArgBP from T. maritima [Thermotoga maritima MSB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9ZF60	1.86e-172	1	309	1	302	Glutamate/aspartate import solute-binding protein OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=gltI PE=3 SV=3
P37902	4.17e-169	1	309	1	302	Glutamate/aspartate import solute-binding protein OS=Escherichia coli (strain K12) OX=83333 GN=gltI PE=1 SV=2
Q9I402	1.14e-109	39	307	29	298	L-glutamate/L-aspartate-binding protein OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA1342 PE=1 SV=1
P27676	1.21e-21	12	242	24	245	Glutamine-binding protein OS=Geobacillus stearothermophilus OX=1422 GN=glnH PE=3 SV=1
O34563	1.48e-21	31	278	30	268	ABC transporter glutamine-binding protein GlnH OS=Bacillus subtilis (strain 168) OX=224308 GN=glnH PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000510	0.997724	0.001079	0.000287	0.000207	0.000171

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002524_01323.