CAZyme Information: MGYG000002525_00564

Basic Information

• Species: Yersinia intermedia
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Yersinia; Yersinia intermedia
• CAZyme ID: MGYG000002525_00564
• CAZy Family: GH23
• CAZyme Description: Soluble lytic murein transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
639	MGYG000002525_2|CGC3	72290.09	9.3852

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002525	5023222	Isolate	Finland	Europe

• Gene Location: Start: 102991; End: 104910 Strand: +

Full Sequence      

MDKWRYLAIGVCLVTTSGAAFADSIDAQRQRYQQVKLAWDSNQMDTVIQLMPTLRDYPLY
PYLEYRQLTQDLSQVSAAQVNDFLSRNPTLPPARSLPSRFVNELARREDWRGLLAFSPTA
PKPVAARCNYYYAKWATGEQQVAWDGASEIWLNGQTLPGSCTKLFSVWQQAGHQTPLATL
ARMKLALKEGNASLVSYLLKQLPADYQTMGTALAKLQSDPASVENFARTVGPTDFTRSAT
TIAFTRLARQDVENARAMIPTLARLQKMSDSEKLELEEAVAWRLMGTDATYDQAKWRDQV
TLRSHSTSLLERRIRMSLGSGDRQGVATWLARLPADAQDKDEWRYWRASLLLEQGKKTEG
EAILRSLMQERGFYPMVAAQKLGVPYPINVEVATKPDASLAQRPEIIRVRELMYWNMDNL
ARNEWSYLVASRSKPEQEALARYAFNQKWADLSVQATIVAKLWDHLEERFPLAWPKEFRQ
ATEGKGITPSYAMAIARQESAWNPKAQSPVGAAGLMQVMPRTAEHTVKLNNISGYVNSSQ
LLDPLTNIEIGTSYLEEVYQQFGRNRILSSAAYNAGPSRVNTWLGNSGGQVDAVAFIESI
PFSETRGYVKNVLAYDAFYRHFMNRPAKVLSDAEWQRRY

Enzyme Prediction     

No EC number prediction in MGYG000002525_00564.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	487	616	7.5e-30	0.7925925925925926

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11619	PRK11619	0.0	1	639	6	644	lytic murein transglycosylase; Provisional
cd13401	Slt70-like	2.91e-63	469	621	1	151	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
COG0741	MltE	8.09e-47	341	628	1	292	Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains) [Cell wall/membrane/envelope biogenesis].
cd16896	LT_Slt70-like	1.54e-40	471	615	1	143	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	2.64e-37	478	590	1	109	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ARB83765.1	0.0	1	639	1	639
AJJ19029.1	0.0	1	639	1	639
AVL37552.1	0.0	1	639	1	639
QGR72740.1	0.0	1	639	1	639
QGR67724.1	0.0	1	639	1	639

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1QSA_A	5.41e-295	23	639	1	618	CrystalStructure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.65 Angstroms Resolution [Escherichia coli],1QTE_A Crystal Structure Of The 70 Kda Soluble Lytic Transglycosylase Slt70 From Escherichia Coli At 1.90 A Resolution In Complex With A 1,6- Anhydromurotripeptide [Escherichia coli]
1SLY_A	1.79e-293	23	639	1	618	ComplexOf The 70-Kda Soluble Lytic Transglycosylase With Bulgecin A [Escherichia coli]
5OHU_A	2.25e-90	3	639	2	638	TheX-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa [Pseudomonas aeruginosa]
6FBT_A	8.19e-89	28	639	1	609	ChainA, Lytic murein transglycosylase [Pseudomonas aeruginosa]
6FCQ_A	2.42e-88	28	639	1	609	ChainA, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCR_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCS_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa],6FCU_A Chain A, Soluble lytic murein transglycosylase [Pseudomonas aeruginosa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0AGC3	4.27e-303	4	639	9	645	Soluble lytic murein transglycosylase OS=Escherichia coli (strain K12) OX=83333 GN=slt PE=1 SV=1
P0AGC4	4.27e-303	4	639	9	645	Soluble lytic murein transglycosylase OS=Escherichia coli O157:H7 OX=83334 GN=slt PE=3 SV=1
P39434	3.11e-298	4	639	9	645	Soluble lytic murein transglycosylase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=slt PE=3 SV=2
P44888	7.28e-75	266	639	220	593	Putative soluble lytic murein transglycosylase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=slt PE=3 SV=1
P39433	2.55e-16	598	639	1	42	Soluble lytic murein transglycosylase (Fragment) OS=Enterobacter cloacae OX=550 GN=slt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000392	0.998817	0.000217	0.000190	0.000193	0.000172

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002525_00564.