CAZyme Information: MGYG000002533_00055

Basic Information

• Species: Vibrio vulnificus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Vibrionaceae; Vibrio; Vibrio vulnificus
• CAZyme ID: MGYG000002533_00055
• CAZy Family: GH19
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
563	MGYG000002533_1|CGC1	61914.78	4.4561

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002533	4933179	Isolate	Bangladesh	Asia

• Gene Location: Start: 60555; End: 62246 Strand: +

Full Sequence      

MKISHLSVLIGCTLAAGAQAAMNIQPDPQNPNGYVVSRVDLQAAEQAQTADPMYAIWSKA
LETRSNTIVEAIAPGAASNPDNVKRTERVFPQSEWDFLTHMAAPEYTYTRFLKAIGKFPA
FCGEYSDGRDSDAICKKSIVTAFAHFAQETGGHIAIDNVSDNPLGLEEWQQALVHVREMG
WSEGQAGYTTGCGQNDWQNKRWPCEAGQGYFGRGAKQLSYHFNYGAFSEVMYDGDATVLL
KNPALVADSWLNLASAIWFFLTPQAPKPAMLHVIDRTWVPSQREVDAGIGYGFGTTINVI
NGGIECGEQNKDKGQPVNRIRYWEGLASHYQIPVEADEKNTCWQQTPYGSLNLNGATDVL
YTNWDGNWKYYADRPGGYSFECELVGFQTAYSALVPGDYEKCVTNFYGSHASWPEVRVVD
KLDPVDPGTQPGGNEWNASKVYVAGDQVTYKGATYKAKWWTQGNDPSLGGPWELVAGTPT
EPTPTPDPVPTPDPTPDPEPTPDPTPDPTPVPGTFIQWEPGVTQVANGEKVTYNGKCFIA
KNGPGVWETPTQSNWFWDEISCQ

Enzyme Prediction     

EC	3.2.1.14	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH19	82	342	1.3e-65	0.9956709956709957
CBM5	435	472	1.5e-17	0.925

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00325	chitinase_GH19	3.78e-60	90	342	1	224	Glycoside hydrolase family 19, chitinase domain. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Glycoside hydrolase family 19 chitinases are found primarily in plants (classes I, III, and IV), but some are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I chitinases, but they are smaller in size due to certain deletions. Despite lacking any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the prokaryotic/eukaryotic divergence.
COG3979	COG3979	2.90e-31	23	225	1	181	Chitodextrinase [Carbohydrate transport and metabolism].
pfam00182	Glyco_hydro_19	3.30e-30	106	323	25	215	Chitinase class I.
cd12215	ChiC_BD	3.75e-15	434	474	1	42	Chitin-binding domain of chitinase C. Chitin-binding domain of chitinase C (ChiC) of Streptomyces griseus and related proteins. Chitinase C is a family 19 chitinase, and consists of a N-terminal chitin binding domain and a C-terminal chitin-catalytic domain that effects degradation. Chitinases function in invertebrates in the degradation of old exoskeletons, in fungi to utilize chitin in cell walls, and in bacteria which use chitin as an energy source. ChiC contains the characteristic chitin-binding aromatic residues.
smart00495	ChtBD3	2.40e-11	435	472	3	41	Chitin-binding domain type 3.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAC93541.1	0.0	1	563	1	563
AMG11349.1	0.0	1	563	1	563
QMV35035.1	0.0	1	563	1	563
ANN27441.1	0.0	1	563	1	563
ALM71745.1	0.0	1	563	1	563

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7V91_A	6.46e-29	106	350	29	244	ChainA, GH19 Chitinase [Ficus microcarpa],7V91_B Chain B, GH19 Chitinase [Ficus microcarpa],7V91_C Chain C, GH19 Chitinase [Ficus microcarpa],7V91_D Chain D, GH19 Chitinase [Ficus microcarpa]
4TX7_A	7.95e-28	106	349	32	245	Crystalstructure of chitinase (GH19) from Vigna unguiculata [Vigna unguiculata subsp. sesquipedalis]
7V92_A	1.49e-27	106	350	29	244	ChainA, GH19 Chitinase [Ficus microcarpa],7V92_B Chain B, GH19 Chitinase [Ficus microcarpa],7V92_C Chain C, GH19 Chitinase [Ficus microcarpa],7V92_D Chain D, GH19 Chitinase [Ficus microcarpa]
2Z37_A	9.48e-27	106	348	29	243	Crystalstructure of Brassica juncea chitinase catalytic module (Bjchi3) [Brassica juncea],2Z37_B Crystal structure of Brassica juncea chitinase catalytic module (Bjchi3) [Brassica juncea],2Z37_C Crystal structure of Brassica juncea chitinase catalytic module (Bjchi3) [Brassica juncea],2Z37_D Crystal structure of Brassica juncea chitinase catalytic module (Bjchi3) [Brassica juncea]
2Z38_A	1.02e-26	106	348	32	246	Crystalstructure of chloride bound Brassica juncea chitinase catalytic module (Bjchi3) [Brassica juncea]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P52404	3.46e-28	106	350	94	309	Endochitinase 2 (Fragment) OS=Solanum tuberosum OX=4113 GN=CHTB2 PE=2 SV=1
P25765	4.20e-28	106	350	108	326	Chitinase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht12 PE=2 SV=2
P52405	1.70e-27	106	350	96	311	Endochitinase 3 (Fragment) OS=Solanum tuberosum OX=4113 GN=CHTB3 PE=2 SV=1
P52403	1.70e-27	106	350	96	311	Endochitinase 1 (Fragment) OS=Solanum tuberosum OX=4113 GN=CHTB1 PE=2 SV=1
P51613	2.15e-27	106	350	92	307	Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000454	0.998750	0.000243	0.000196	0.000173	0.000162

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002533_00055.