CAZyme Information: MGYG000002534_02826

Basic Information

• Species: Citrobacter_A farmeri
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter_A; Citrobacter_A farmeri
• CAZyme ID: MGYG000002534_02826
• CAZy Family: GH37
• CAZyme Description: Periplasmic trehalase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
568	MGYG000002534_1|CGC40	63727.74	5.2888

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002534	5715629	Isolate	Australia	Oceania

• Gene Location: Start: 2944032; End: 2945738 Strand: +

Full Sequence      

MPTPVFRASASLSLAITLAVSGTLLTFSSLSVNAEEMPDSPPQPPDILLGPLFNDVQNAR
LFPDQKTFADAVPNSDPLMILADYRMQKNQFSFDLRHFVSVNFTLPKEGEKYVPPKGQSL
REHIDGLWPVLTRSAESAGKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESDRWD
KIADMVANFAWEIDTFGHIPNGNRTYYLSRSQPPFFSLMVELLAQHDGDDALKKYLPQLQ
KEYTYWMEGVETLQAGQQNKRVVKLDDGTILNRYWDERDTPRPESWVEDIATAKSNPNRP
ATEIYRDLRSAAASGWDFSSRWMDNPQQLGTLRTTSIVPVDLNALLYKMEKILARASKAA
GDEAKASQYDTLASARQKAIEHYLWNDKEGWYADYDLKSKRVRNQLTAAALFPLYVNAAS
KDRASKMAAATRAHLLQPGGLATTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQNDVAME
VSWRFLTNVQHTYDREQKLVEKYDVSSTGTGGGGGEYPLQDGFGWSNGVTLKMLDLICPK
EKPCDSVPEKRPVAGQTSTEPPQKQATP

Enzyme Prediction     

EC	3.2.1.28

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH37	61	536	9.8e-191	0.9959266802443992

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK13270	treF	0.0	34	536	31	545	alpha,alpha-trehalase TreF.
PRK13272	treA	0.0	3	540	5	539	alpha,alpha-trehalase TreA.
COG1626	TreA	0.0	18	543	28	558	Neutral trehalase [Carbohydrate transport and metabolism].
PRK13271	treA	0.0	1	568	1	567	alpha,alpha-trehalase TreA.
pfam01204	Trehalase	0.0	60	537	1	508	Trehalase. Trehalase (EC:3.2.1.28) is known to recycle trehalose to glucose. Trehalose is a physiological hallmark of heat-shock response in yeast and protects of proteins and membranes against a variety of stresses. This family is found in conjunction with pfam07492 in fungi.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AST80212.1	0.0	1	568	1	568
QZE47382.1	0.0	1	568	1	568
QFS71596.1	0.0	1	568	1	568
AUO64200.1	0.0	1	568	1	568
AGE94334.1	0.0	1	568	1	568

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JF4_A	0.0	35	568	1	533	Family37 trehalase from Escherichia coli in complex with validoxylamine [Escherichia coli K-12]
2JG0_A	0.0	35	568	1	533	Family37 trehalase from Escherichia coli in complex with 1- thiatrehazolin [Escherichia coli K-12],2JJB_A Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_B Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_C Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2JJB_D Family 37 trehalase from Escherichia coli in complex with casuarine-6- O-alpha-glucopyranose [Escherichia coli K-12],2WYN_A Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_B Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_C Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12],2WYN_D Structure of family 37 trehalase from Escherichia coli in complex with a casuarine-6-O-a-D-glucoside analogue [Escherichia coli K-12]
5Z66_A	0.0	45	554	47	556	Structureof periplasmic trehalase from Diamondback moth gut bacteria complexed with validoxylamine [Enterobacter cloacae],5Z6H_A Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae],5Z6H_B Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form [Enterobacter cloacae]
7E9U_A	1.98e-83	94	535	93	552	ChainA, Trehalase [Arabidopsis thaliana],7E9U_B Chain B, Trehalase [Arabidopsis thaliana]
7E9X_A	2.94e-82	94	535	93	552	ChainA, Trehalase [Arabidopsis thaliana],7E9X_B Chain B, Trehalase [Arabidopsis thaliana],7E9X_C Chain C, Trehalase [Arabidopsis thaliana],7E9X_D Chain D, Trehalase [Arabidopsis thaliana],7EAW_A Chain A, Trehalase [Arabidopsis thaliana],7EAW_B Chain B, Trehalase [Arabidopsis thaliana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B7LGV7	0.0	25	568	21	563	Periplasmic trehalase OS=Escherichia coli (strain 55989 / EAEC) OX=585055 GN=treA PE=3 SV=1
B7NUW3	0.0	25	568	21	563	Periplasmic trehalase OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) OX=585057 GN=treA PE=3 SV=1
B7N408	0.0	25	568	21	563	Periplasmic trehalase OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) OX=585056 GN=treA PE=3 SV=1
B7LXB1	0.0	25	568	21	563	Periplasmic trehalase OS=Escherichia coli O8 (strain IAI1) OX=585034 GN=treA PE=3 SV=1
C4ZTN8	0.0	25	568	21	563	Periplasmic trehalase OS=Escherichia coli (strain K12 / MC4100 / BW2952) OX=595496 GN=treA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.004520	0.993224	0.000577	0.001163	0.000272	0.000211

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002534_02826.