CAZyme Information: MGYG000002536_02123

Basic Information

• Species: Plesiomonas shigelloides
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Plesiomonas; Plesiomonas shigelloides
• CAZyme ID: MGYG000002536_02123
• CAZy Family: GH102
• CAZyme Description: Membrane-bound lytic murein transglycosylase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
385	MGYG000002536_22|CGC4	42146.91	8.9952

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002536	3653255	Isolate	not provided	not provided

• Gene Location: Start: 264958; End: 266115 Strand: -

Full Sequence      

MRPVFARFTASAEATVARATYSLRPSRCGIGLMACLLLALAGCAPKAPPTDRGQQYKDGE
ITQPLKPVAQVNSRLLPQNQNEFSQQVENVKFGSPSLHFRQQHTYADIYRWLDAGADPRK
LAQFGLQPYLMAGGDNQGNVLFTGYYTPVLQARHKPDAVYRYPLYALPKGNARFTRAEIY
NGALQGKGLELAYSASMLDNFMMEVQGSGYVDFGNGEPLAYFGYAGKNGYAYTSIGRVLI
DRGEVPREQMSLDAIRKWANTHTPQQVQELFEQNQSYVYFEPRAAAPVVGASGIPLVAKA
SVAGDKSLIPPGSVLLAEVPLLDGQGRFTGKHEMRLMLALDVGGAVKGNHFDIYHGVGDD
AGKEAGFYQHFGRVWVLKSPADGRL

Enzyme Prediction     

No EC number prediction in MGYG000002536_02123.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	173	281	1.9e-40	0.6751592356687898

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11162	mltA	0.0	49	378	25	355	murein transglycosylase A; Provisional
COG2821	MltA	8.52e-141	46	378	28	364	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
smart00925	MltA	4.38e-60	149	280	1	152	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
pfam03562	MltA	2.83e-55	112	280	37	230	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
cd14668	mlta_B	7.63e-55	150	283	1	159	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SBT61890.1	1.35e-284	1	385	1	385
QOH81267.1	1.20e-261	33	385	1	353
QIY10509.1	1.20e-261	33	385	1	353
QWK95831.1	1.20e-261	33	385	1	353
QWK98472.1	2.42e-261	33	385	1	353

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2AE0_X	7.82e-138	49	385	5	342	Crystalstructure of MltA from Escherichia coli reveals a unique lytic transglycosylase fold [Escherichia coli]
2GAE_A	8.10e-138	49	385	6	343	Crystalstructure of MltA from E. coli [Escherichia coli]
2PJJ_A	1.28e-136	49	385	5	342	ChainA, Membrane-bound lytic murein transglycosylase A [Escherichia coli K-12]
2PIC_A	2.57e-136	49	385	5	342	ChainA, Membrane-bound lytic murein transglycosylase A [Escherichia coli K-12]
2PI8_A	7.39e-131	49	385	5	342	Crystalstructure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_B Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_C Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12],2PI8_D Crystal structure of E. coli MltA with bound chitohexaose [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0A935	4.53e-139	32	385	10	362	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	4.53e-139	32	385	10	362	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
Q9KPQ4	1.62e-117	37	377	11	349	Membrane-bound lytic murein transglycosylase A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mltA PE=3 SV=1
P57531	3.53e-101	51	377	28	356	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1
Q8K9A7	1.51e-93	50	378	35	365	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001504	0.357094	0.641282	0.000093	0.000030	0.000009

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002536_02123.