CAZyme Information: MGYG000002547_02056

Basic Information

• Species: Megasphaera elsdenii
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Veillonellales; Megasphaeraceae; Megasphaera; Megasphaera elsdenii
• CAZyme ID: MGYG000002547_02056
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
262	MGYG000002547_18|CGC1	29139.31	9.3156

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002547	2429033	Isolate	India	Asia

• Gene Location: Start: 3735; End: 4523 Strand: +

Full Sequence      

MILSKACKTKMAAFLLAVMALAPVQAITQEEMVYDQVSKYIRGSEAAAIAQDICDASYMY
DVDPILVTAVFTTESHFDNSAVSGVGAVGIAQLMPATAASLNVNPYNRRENIFGGVKYLG
QMVERYRTWDKPFVYAEAAYNAGPGAVDRARGVPHYTETMNYVQTVEQTRREIWRLAGHE
AMAMQPYKRSAMTMQEKEAPIFPSLKEWHEQHSQKAAQVQAKSVGSTKPALVRTKAARVA
PEHPRPSYWTTSLPWGEGTRRK

Enzyme Prediction     

No EC number prediction in MGYG000002547_02056.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	63	170	9e-22	0.7851851851851852

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00254	LT-like	5.03e-32	64	166	1	106	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13403	MLTF-like	1.31e-25	55	151	3	103	membrane-bound lytic murein transglycosylase F (MLTF) and similar proteins. This subfamily includes membrane-bound lytic murein transglycosylase F (MltF, murein lyase F) that degrades murein glycan strands. It is responsible for catalyzing the release of 1,6-anhydromuropeptides from peptidoglycan. Lytic transglycosylase catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc) as do goose-type lysozymes. However, in addition, it also makes a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	5.14e-25	56	166	13	142	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
cd16896	LT_Slt70-like	3.82e-23	51	171	6	145	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
pfam01464	SLT	4.03e-21	56	152	4	104	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCC73807.1	3.03e-185	1	262	1	262
AVO28419.1	1.52e-178	11	262	1	252
AVO75648.1	1.52e-178	11	262	1	252
ALG42819.1	1.81e-175	19	262	1	244
ALG42693.1	3.62e-74	20	183	5	168

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MPQ_A	5.29e-06	61	166	428	548	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
5O1J_A	5.30e-06	61	166	432	552	Lytictransglycosylase in action [Neisseria meningitidis MC58]
6FPN_B	5.32e-06	61	166	438	558	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	5.33e-06	61	166	442	562	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	5.34e-06	61	166	448	568	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31608	6.94e-26	48	166	59	175	Putative murein lytic transglycosylase YjbJ OS=Bacillus subtilis (strain 168) OX=224308 GN=yjbJ PE=3 SV=1
O31976	1.21e-22	43	166	1416	1537	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
O64046	1.21e-22	43	166	1416	1537	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1
P27380	2.80e-11	56	126	14	85	Transglycosylase OS=Enterobacteria phage PRD1 OX=10658 GN=VII PE=1 SV=3
Q0A9W6	5.92e-08	55	151	298	398	Membrane-bound lytic murein transglycosylase F 1 OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=mltF1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000244	0.999119	0.000164	0.000173	0.000158	0.000142

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002547_02056.