CAZyme Information: MGYG000002567_00286

Basic Information

• Species: CAG-485 sp900547755
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900547755
• CAZyme ID: MGYG000002567_00286
• CAZy Family: GH0
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
750	MGYG000002567_3|CGC1	83456.63	5.8798

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002567	2236504	MAG	China	Asia

• Gene Location: Start: 56526; End: 58778 Strand: +

Full Sequence      

MKKELLLFIGMAALPLAASADIVRTSVYRWHADTVSQGEYKAVAVSPNRIESTYKAQPGY
FMPVNGVWQKQHDLSAYPQLFTPNKLHTAVYNMGLDEMVNAVEPDTTLRTGKEWAGVWTR
DVSYSILLSMAYMQPEASRISLEKKVDRLGRIIQDTGSGGAWPVSSDRQIWTVAAWEVYK
VTGDKEWLRFAFDVARRSLLTDEQTVYDPATGLVRGETSFIDWREQSHPKWMQCADIYST
LTLSTSIVHSEAWKSLAGMAKALGRKDAAAYEARARRIDEAVNKYLWMPDKGYYAMYRYG
RDNQILNPRAETLGESLAIVWGVADRQRAVSITENNPVTPFGPGVFFPQIPDVPAYHNNS
LWPWVDAWWTIANAKAGNEQGVMQGFGALVRPAALFCTNKENFVLDNGDIATELNSSNML
WCLAGNIAITHKVLFGIGFESDGLSFAPFVPEVMGQSRTLSNFKYRNAILNITVEGYGSK
IKSFTVNGKEQKPFLPASKAKGNMDIRIVMDCEPIPALKVNESTNVKTPITPIARIEGDR
LVWQSIEYINHYIVLRDGNPVAETRSTAYTMIEPGEYQIIGVSDDGVQSFASEPMSNRLR
VDVQMPDEGTAVKSAEIPADYRPSNGVKGYEGAGFVETDKSSGPVNVKVYLPEGGEWFLT
LRYANGNGPVNTENRCAIRTVSVDGNRVGLAVLPHRGRGNWDDWGISNSLLLPALLPGEH
TVTVEYLPEDENMNIDTNHALLDRMTLTKK

Enzyme Prediction     

No EC number prediction in MGYG000002567_00286.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04083	CBM35_Lmo2446-like	3.24e-15	629	737	22	122	Carbohydrate Binding Module 35 (CBM35) domains similar to Lmo2446. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes. Some CBM35 domains belonging to this family are appended to glycoside hydrolase (GH) family domains, including glycoside hydrolase family 31 (GH31), for example the CBM35 domain of Lmo2446, an uncharacterized protein from Listeria monocytogenes EGD-e. These CBM35s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. GH31 has a wide range of hydrolytic activities such as alpha-glucosidase, alpha-xylosidase, 6-alpha-glucosyltransferase, or alpha-1,4-glucan lyase, cleaving a terminal carbohydrate moiety from a substrate that may be a starch or a glycoprotein. Most characterized GH31 enzymes are alpha-glucosidases.
cd04082	CBM35_pectate_lyase-like	6.71e-08	629	736	21	120	Carbohydrate Binding Module family 35 (CBM35), pectate lyase-like; appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. This family includes carbohydrate binding module family 35 (CBM35) domains that are non-catalytic carbohydrate binding domains that are appended mainly to enzymes that bind mannan (Man), xylan, glucuronic acid (GlcA) and possibly glucans. Included in this family are CBM35s of pectate lyases, including pectate lyase 10A from Cellvibrio japonicas, these enzymes release delta-4,5-anhydrogalaturonic acid (delta4,5-GalA) from pectin, thus identifying a signature molecule for plant cell wall degradation. CBM35s are unique in that they display conserved specificity through extensive sequence similarity but divergent function through their appended catalytic modules. They are known to bind alpha-D-galactose (Gal), mannan (Man), xylan, glucuronic acid (GlcA), a beta-polymer of mannose, and possibly glucans, forming four subfamilies based on general ligand specificities (galacto, urono, manno, and gluco configurations). In contrast to most CBMs that are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. Some CBM35s bind their ligands in a calcium-dependent manner, especially those binding uronic acids.
COG3408	GDB1	4.89e-07	114	384	281	559	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
cd02795	CBM6-CBM35-CBM36_like	6.48e-06	629	736	20	120	Carbohydrate Binding Module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules. This alignment model also contains the C-terminal domains of bacterial insecticidal toxins, where they may be involved in determining insect specificity through carbohydrate binding functionality.
cd04086	CBM35_mannanase-like	0.003	624	725	16	105	Carbohydrate Binding Module 35 (CBM35); appended to several carbohydrate binding enzymes, including several glycoside hydrolase (GH) family 26 mannanase domains. This family includes carbohydrate binding module 35 (CBM35) domains that are appended to several carbohydrate binding enzymes, including periplasmic component of ABC-type sugar transport system involved in carbohydrate transport and metabolism, and several glycoside hydrolase (GH) domains, including GH26. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB35s belonging to this family are mannanase A from Clostridium thermocellum (GH26), Man26B from Paenibacillus sp. BME-14 (GH26), and the multifunctional Cel44C-Man26A from Paenibacillus polymyxa GS01 (which has two GH domains, GH44 and GH26). GH26 mainly includes mannan endo-1,4-beta-mannosidase which hydrolyzes 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans, and galactoglucomannans, but displays little activity towards other plant cell wall polysaccharides. A few proteins belonging to this family have additional CBM3 domains; these CBM3s are not found in the CBM6-CBM35-CBM36_like superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AWG27199.1	9.69e-220	66	749	213	892
QNK61875.1	5.46e-218	68	743	216	884
QEC58493.1	1.26e-214	68	749	199	876
AMQ00027.1	3.81e-214	68	749	200	874
QEC42842.1	1.46e-213	68	747	217	892

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000252	0.999061	0.000181	0.000175	0.000157	0.000147

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002567_00286.