CAZyme Information: MGYG000002570_00086

Basic Information

• Species: Ruminococcus_E sp003526955
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; Ruminococcus_E; Ruminococcus_E sp003526955
• CAZyme ID: MGYG000002570_00086
• CAZy Family: GH23
• CAZyme Description: DNA mismatch repair protein MutS

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2563	MGYG000002570_2|CGC1	292476.67	4.7447

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002570	2026568	MAG	China	Asia

• Gene Location: Start: 19832; End: 27523 Strand: +

Full Sequence      

MARKFDRISQLYEDSSKAVMSPDKWAAFLKTACHNYRLRFDEQLLLYAQKPDATAVLPIE
QWNRTFGRWVNRGAKGIAVFESLNGNSQRLVHYFDISDTHASEISRAVPIWNMKPEYTDE
VIDTLESSFGYLDEKNSLADAIISASGNAVEDNITDYVGDLLVTVNNTFLEELDENIVKS
MYRQLVSNSVAFMIMTRLGIDTEPYFNDDDFRDIINFNTQEALNALGFATSDIAEMGLSE
ISKTVLALNRQNRIIAENRQSEYTNSTINTERSLNNGRTDIHNAGRLPASEPDASRTAEG
TTRTLRSDEKELPVAETQNSLLQSFDEGNADRTPVGNRTDGIRDGGQNSQTDGGAGVRDR
EAESGRPDEVDTTDEQPQTLGSGDRQSGSDLRLEYYDRSNEDKSIPFFGSDDKIKAILLS
APYLKATKTEIARYFENESDSEKRVEYLKSIFNNDATELTTPEGKRLGYKTYQNVLLLWE
GEQENKISQAYYDWGVIAGYFEGMRLLGELRDTIKPLPSLEGQLEILTAEAGEKTPAFVF
SQEIIDAVLTHGSGVSEGKMRIYEQFQKSLSAKENIDFLKNEYGWGGSYPIIIGTGIDES
HDGKGIMLSRGFEDNAPRLLLNWSKVEKRIGELIKLDRYLNPKEREYYPTWLEKQEQIRA
ERAAEKEIREAIYTPPAEKEAVPVEYEYKYSLGASVYIGADEYEILSFDENRVMLFDKQF
PLFNKEMTRTEFDSKVKENPMNDHLKVAVQPEKEKQITKRDYSRYSPVMQEYFKIKDEHP
DTIILFQIGDFYEAMGDDAVTVSQALSLHNTTRRITDTERIDMCGFPSHRLETYADMLLD
RGYNIGISSPNERESRDFILMTSDRAHAPIQSEPIGGIDNSDLIGKELTLDNRNFIVERI
DRNGDASMRDITFEDSNSFPIFRVEKADFVRSALELYEKKELDAPVWERKQKAKAQTFDL
HPEIPLADRYTFDLKNFKLEEVGKKARFRRNTEAIRVLKQCEEGNRFATPEEQQLLAQYV
GWGGIPEAFDENNATWQSEYNELSQLLSDEEYQSARESTLTAFYTPPVVISAIYKVMEQM
EFKEGNILEPSCGIGNFIGMLPQSMQNSRVYGVEIDKISAGIAQQLYQRSSIAGQPFEQA
EIPDSFFDAVVGNVPFGDFSVADKRYDKNHFLIHDYFFAKSLDKLRPGGVMALVTSKGTM
DKENPAVRKYIAQRAELLGAIRLPNDTFKGNAGTEVVSDILILQKHDRIIDIEPDWVHLN
TDKNGIRMNSYFAEHPEMILGDMKIVSGRFGEETTCVPYENADLESLLDEAVSNIHGEIA
EYETEENIEEEDNSIPADPNVRNFSYTVLDDVIYFRENSRMSPVEVSATAENRIKGLIEI
RNSVRTLLDLQTEDYPDSDIKAEQERLNKLYDGFTKKYGLINSRANTMAFSQDSSYSLLS
SLEILDDEGNLERKADMFFKRTIKPHTPVTFVDTASEALAVSMGEKAGIDMEYMCQLSGK
SEQEVFEELKGVIFLNSLYGQGNPNEQKYLMADEYLSGNVREKLEVARRSAKLYPDDYNI
NVEALERVQPKDLTASEISVRLGATWIPPEIVQQFIYEFLDTPRYAQWKIRLHYSPYTSE
WSIENKSYDTSNLKVYRTYGTDRINAYKIIEETLNLKDVRIFDYVEDEEGRKKPVLNKKE
TTIALQKQELIKEGFKEWIWKDPQRREKLCKLYNEKFNSTRPREYDGSHITFSGMNPEIE
LREHQKNAVAHILYGGNTLLAHAVGAGKTYEMTAAAMESKRLGLCSKSLFVVPNHLTEQW
AAEFLQLYPAANILVATKKDFETKNRKRFCGRIATGDYDAIIIGHSQFEKIPMSVERQRI
ILEQQIDEITDGIRELKENRGENFSIKQLEKSRKSLKVKLQKLNDQSRKDDVVTFEELGV
DRLFIDESHYYKNLYLYTKMRNVGGIAQTEAQKSSDLFMKCRYLDEITGGRGTVFATGTP
ISNSMVELYTIQRYLQYETLVENGLQHFDSWASTFGETVTAYELKPEGTGYRTKTRFAKF
YNLPELMAMFKEVADIKTADTLNLPTPTPHYHNVAVKPSDFQKAMVAELGERAEKVRNNQ
VDASVDNMLKITNDGRKLALDQRMINSLLPDFESSKINACIDNIFMIWQENADKKSAQLV
FCDLSTPKPDAEFSVYTDIRKKLIERGVPKSEISFIHEAKTEAQKQELFKKVRKGEVRVL
LGSTQKMGAGTNVQNKLIALHDIDCPWRPSDLEQRSGRIIRQGNENPDVDIYRYVTEETF
DAYLYQLVEGKQKFASQIMTSKSPVRSAEDIDDTALSYAEIKMLATGNPYIKEKMDLDIQ
VQKLRLYKSHYLSEKYELEDKIIKFYPQEISLLTARVDGLKADLEIAKAHPKEVDGKFAG
MVIDGYSYTEKAEAGQVIIEACKAKTTSEPTPLGEYRGFKMELEFDTFERAYAVKLKGHF
THSVTLGTDVYGNITRIDNAVDNIEARLHKAEEQLENTKAQFETAKVEVEKPFAQEDELK
QKMARLTELNALLDMDKGDTPVVGGEPDEGDLPPTRSRDDRER

Enzyme Prediction     

No EC number prediction in MGYG000002570_00086.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4646	COG4646	2.75e-76	1268	1795	1	540	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
COG4646	COG4646	1.20e-28	1936	2127	439	637	Adenine-specific DNA methylase, N12 class [Replication, recombination and repair].
pfam01624	MutS_I	4.51e-27	767	847	2	82	MutS domain I. This domain is found in proteins of the MutS family (DNA mismatch repair proteins) and is found associated with pfam00488, pfam05188, pfam05192 and pfam05190. The MutS family of proteins is named after the Salmonella typhimurium MutS protein involved in mismatch repair; other members of the family included the eukaryotic MSH 1,2,3, 4,5 and 6 proteins. These have various roles in DNA repair and recombination. Human MSH has been implicated in non-polyposis colorectal carcinoma (HNPCC) and is a mismatch binding protein. The aligned region corresponds with globular domain I, which is involved in DNA binding, in Thermus aquaticus MutS as characterized in.
COG0249	MutS	1.81e-21	761	847	2	83	DNA mismatch repair ATPase MutS [Replication, recombination and repair].
TIGR01070	mutS1	1.11e-18	766	847	2	83	DNA mismatch repair protein MutS. [DNA metabolism, DNA replication, recombination, and repair]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASV45029.1	0.0	972	2543	1548	3187
QIW86704.1	0.0	972	2543	1616	3255
QIW86628.1	0.0	972	2543	1616	3255
AEY69616.1	3.05e-314	983	2492	1700	3294
AXF51455.1	3.30e-313	983	2543	1793	3437

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5AKB_A	4.86e-12	761	848	6	93	MutSin complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKB_B MutS in complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKB_E MutS in complex with the N-terminal domain of MutL - crystal form 1 [Escherichia coli K-12],5AKC_A MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_B MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_E MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_F MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_I MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKC_J MutS in complex with the N-terminal domain of MutL - crystal form 2 [Escherichia coli K-12],5AKD_A MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_B MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_E MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_F MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_I MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12],5AKD_J MutS in complex with the N-terminal domain of MutL - crystal form 3 [Escherichia coli K-12]
7AI6_A	5.05e-12	761	848	6	93	ChainA, DNA mismatch repair protein MutS [Escherichia coli K-12],7AI6_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIB_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIB_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIC_A Chain A, DNA mismatch repair protein MutS [Escherichia coli K-12],7AIC_B Chain B, DNA mismatch repair protein MutS [Escherichia coli K-12]
3K0S_A	6.37e-12	761	847	5	91	ChainA, DNA mismatch repair protein mutS [Escherichia coli K-12],3K0S_B Chain B, DNA mismatch repair protein mutS [Escherichia coli K-12]
1OH5_A	6.38e-12	761	847	6	92	THECRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH5_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A C:A MISMATCH [Escherichia coli],1OH6_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH6_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN A:A MISMATCH [Escherichia coli],1OH7_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH7_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:G MISMATCH [Escherichia coli],1OH8_A THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1OH8_B THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE [Escherichia coli],1W7A_A ATP bound MutS [Escherichia coli],1W7A_B ATP bound MutS [Escherichia coli],2WTU_A Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],2WTU_B Crystal structure of Escherichia coli MutS in complex with a 16 basepair oligo containing an A.A mismatch. [Escherichia coli],3ZLJ_A CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12],3ZLJ_B CRYSTAL STRUCTURE OF FULL-LENGTH E.COLI DNA MISMATCH REPAIR PROTEIN MUTS D835R MUTANT IN COMPLEX WITH GT MISMATCHED DNA [Escherichia coli K-12]
1NG9_A	6.38e-12	761	847	6	92	ChainA, DNA mismatch repair protein MutS [Escherichia coli],1NG9_B Chain B, DNA mismatch repair protein MutS [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q71TF8	1.47e-37	991	2312	51	1567	Defense against restriction protein B OS=Escherichia phage P1 OX=2886926 GN=darB PE=3 SV=1
Q38YR4	3.59e-15	763	852	5	94	DNA mismatch repair protein MutS OS=Latilactobacillus sakei subsp. sakei (strain 23K) OX=314315 GN=mutS PE=3 SV=1
A5VIW9	2.43e-14	766	852	6	92	DNA mismatch repair protein MutS OS=Limosilactobacillus reuteri (strain DSM 20016) OX=557436 GN=mutS PE=3 SV=1
B2G6E5	2.43e-14	766	852	6	92	DNA mismatch repair protein MutS OS=Limosilactobacillus reuteri (strain JCM 1112) OX=557433 GN=mutS PE=3 SV=1
Q03R33	3.22e-14	766	852	7	93	DNA mismatch repair protein MutS OS=Levilactobacillus brevis (strain ATCC 367 / BCRC 12310 / CIP 105137 / JCM 1170 / LMG 11437 / NCIMB 947 / NCTC 947) OX=387344 GN=mutS PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000084	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002570_00086.