CAZyme Information: MGYG000002580_00435

Basic Information

• Species: Lachnospira sp000436475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnospira; Lachnospira sp000436475
• CAZyme ID: MGYG000002580_00435
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1285	MGYG000002580_2|CGC5	136590.75	6.2584

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002580	3016685	MAG	China	Asia

• Gene Location: Start: 100964; End: 104821 Strand: +

Full Sequence      

MKANISKGARKGWAWILAALMVVSVIIPTTISTAKAEVGTVQFNGTAQGWLESAYAEWSI
DKEADGYTAYIKKASQSDSAYARIDNELIRRYKSYYRVDAMGLSAGDYVIRVVPVKNGKE
VTAKAKVTDVLKVSSYDRSGFAFSSESRYKTGSGAYNEDGTLKSDSIVLYVTNDNAKTVK
AGIMEGKGEKEYTGLQTIIDAYTKSASKGVETRALDVRVIGCLTDSGMDKFSSSSEGVQI
KGASAYSNINMTFEGVGEDASISGFGFLLRNAGNVEMRNLSILNFMDDGISLDTANCNVW
IHNVDLYYGKVGGDADQAKGDGSIDIKGDSQYITVSYVHFYDSGKCSLCGMKSESGPNYI
TYHHNWFDHSDSRHARVRTMSVHMYNNYYDGNAKYGVGSTMGSSVYVENNYFRNCKYPML
SSNQGSDALGEGTFSGENGGIIKAYGNEIVGATGIIYANAASVTGEAANAATFDAYLAAS
AGEKVPSSYKTVAGGTTYDNFDTTKDLGVKAGSLVAAKDVPALVTSSKGAGSLGGGVIDW
KFSDEDDTAYVVDKDLKAAVTNYKNTELVAVGGTNANIVTPEPSTDETTKATEEETKTTE
KATTSKATEKETKTTEKATTSKATEATKATEKETTGAGSDTKNSYDKTSLSYSGAYTDLS
TKKDSDFKNAKYVSSASDILTAISSAKAGDVIIVKAGTYKFTDTIVINNAMNGKSGAYIV
VKAADGAEVKFDFSAQTLDGANRGVVVDGDYWYFEGINFYGAGDNGVLLAGNNNIFEKCV
FEANRDSGLQISRYDITAATKDLWPSNNLIINCTAFDNCDYPDQGGTGENADGFAAKLTC
GEGNVFDGCISYCNSDDGWDLFAKSATGPIGVITIRNCVSFGNGTLSNGVHYANGDMNGF
KLGGSGVGTPHVVLNCLSFNNGATGFTDNNNPSGLTIMNATAWGNGKSAKKGNFLCYRAS
NKAVYVGLVSANAIDSDKFTGNMSDSIYYNTGKYYRLSGLLPTPVVSGDKKGTVVDNPSK
VSGMFKNAVDFINLNGNVDKQLRNSDGTINMKGLYETQGSYSTLGAHFGVANQVISVTGK
TSGEVKPTEKTTKATEKTTEKTTETTKATEKTTETTKATEKTTEATKATESKTDSTVSGT
KLVLNANDVNTGKLKSTLTVDGFKIVAGSKATVTVDKSRKEYKGIDFIRRIKLSGKGSTS
GRAIAFKTAGEAKVKVYATSSSTDESRLIKIVDKNGKDITSTSKIPGNSLKSVSFRVPVA
GEYYLIAEDGGVNIYYVEVSNGVAY

Enzyme Prediction     

EC	4.2.2.2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	658	1059	2.2e-112	0.9706666666666667
PL1	247	414	4.2e-43	0.8168316831683168

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	3.22e-51	101	418	1	279	Pectate lyase [Carbohydrate transport and metabolism].
smart00656	Amb_all	2.90e-33	250	416	17	190	Amb_all domain.
pfam00544	Pec_lyase_C	1.81e-15	249	412	34	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
cd14251	PL-6	1.49e-07	673	814	1	134	Polysaccharide Lyase Family 6. Polysaccharide Lyase Family 6 is a family of beta-helical polysaccharide lyases. Members include alginate lyase (EC 4.2.2.3) and chondroitinase B (EC 4.2.2.19). Chondroitinase B is an enzyme that only cleaves the beta-(1,4)-linkage of dermatan sulfate (DS), leading to 4,5-unsaturated dermatan sulfate disaccharides as the product. DS is a highly sulfated, unbranched polysaccharide belonging to a family of glycosaminoglycans (GAGs) composed of alternating hexosamine (gluco- or galactosamine) and uronic acid (D-glucuronic or L-iduronic acid) moieties. DS contains alternating 1,4-beta-D-galactosamine (GalNac) and 1,3-alpha-L-iduronic acid units. The related chondroitin sulfate (CS) contains alternating GalNac and 1,3-beta-D-glucuronic acid units. Alginate lyases (known as either mannuronate (EC 4.2.2.3) or guluronate lyases (EC 4.2.2.11) catalyze the degradation of alginate, a copolymer of alpha-L-guluronate and its C5 epimer beta-D-mannuronate.
pfam14592	Chondroitinas_B	2.67e-04	673	787	1	117	Chondroitinase B. This family includes chondroitinases. These enzymes cleave the glycosaminoglycan dermatan sulfate.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACR72247.1	0.0	1	1285	1	1286
ACR72246.1	0.0	1	1284	1	1472
QMW93302.1	3.58e-269	47	1077	60	1104
BBK78741.1	3.58e-269	47	1077	60	1104
AOR96287.1	1.75e-266	47	1077	60	1104

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RU4_A	1.03e-36	682	946	41	293	ChainA, Pectate lyase [Dickeya chrysanthemi]
3VMV_A	2.12e-24	170	414	7	248	Crystalstructure of pectate lyase Bsp165PelA from Bacillus sp. N165 [Bacillus sp. N16-5],3VMW_A Crystal structure of pectate lyase Bsp165PelA from Bacillus sp. N165 in complex with trigalacturonate [Bacillus sp. N16-5]
1VBL_A	1.35e-18	246	412	129	330	Structureof the thermostable pectate lyase PL 47 [Bacillus sp. TS-47]
5OLQ_A	3.56e-17	767	948	140	322	Rhamnogalacturonanlyase [Bacteroides thetaiotaomicron],5OLQ_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLQ_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLS_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron]
1PCL_A	7.94e-15	204	412	37	276	ChainA, PECTATE LYASE E [Dickeya chrysanthemi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C1A6	1.12e-36	665	946	49	318	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	9.18e-36	682	946	66	318	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P22751	6.38e-25	678	945	410	639	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
Q8GCB2	2.52e-24	193	421	67	281	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1
B1B6T1	2.52e-24	193	421	67	281	Pectate trisaccharide-lyase OS=Bacillus sp. OX=1409 GN=pel PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000325	0.998990	0.000179	0.000179	0.000154	0.000144

TMHMM  Annotations     

start	end
12	34