dbCAN-seq: a database of CAZyme sequence and annotation

Basic Information help

Species

Lineage

Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides;

CAZyme ID

MGYG000002585_00260

CAZy Family

GH99

CAZyme Description

hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
413	MGYG000002585_3\|CGC1	45459.83	4.9715

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002585	2701793	MAG	China	Asia

Gene Location

Start: 20458; End: 21699 Strand: +

Enzyme Prediction help

No EC number prediction in MGYG000002585_00260.

CAZyme Signature Domains help

Family	Start	End	Evalue	family coverage
GH99	52	374	8.3e-67	0.9880239520958084

CDD Domains download full data without filtering help

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd11575	GH99_GH71_like_3	9.02e-138	44	405	1	376	Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.
cd11573	GH99_GH71_like	4.56e-27	104	372	5	282	Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.
cd11574	GH99	5.60e-23	56	350	5	319	Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase. This family of glycoside hydrolases 99 (following the CAZY nomenclature) includes endo-alpha-1,2-mannosidase (EC 3.2.1.130), which is an important membrane-associated eukaryotic enzyme involved in the maturation of N-linked glycans. Specifically, it cleaves mannoside linkages internal to N-linked glycan chains by hydrolyzing an alpha-1,2-mannosidic bond between a glucose-substituted mannose and the remainder of the chain. The biological function and significance of the soluble bacterial orthologs, which may have obtained the genes via horizontal transfer, is not clear.
pfam16317	Glyco_hydro_99	1.01e-13	49	342	1	308	Glycosyl hydrolase family 99. This domain, around 350 residues, is mainly found in some uncharacterized proteins from bacteroides to human. Some proteins in this family, annotated as endo-alpha-mannosidases cleave mannoside linkages internally within an N-linked glycan chain, short circuiting the classical N-glycan biosynthetic pathway. This domain reveals a (beta-alpha)(8) barrel fold in which the catalytic centre is present in a long substrate-binding groove, consistent with cleavage within the N-glycan chain, providing a foundation upon which to develop new enzyme inhibitors targeting the hijacking of N-glycan synthesis in viral disease and cancer.
cd11578	GH99_GH71_like_1	1.82e-07	54	356	3	308	Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.

CAZyme Hits help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCO21487.1	8.57e-149	40	413	39	408
CCO21838.1	8.57e-149	40	413	39	408
CCO21727.1	8.57e-149	40	413	39	408
CCO21747.1	8.57e-149	40	413	39	408
CCO21767.1	8.57e-149	40	413	39	408

PDB Hits download full data without filtering help

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ACZ_A	3.91e-13	4	342	8	339	Structureof the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],4AD0_A Structure of the GH99 endo-alpha-mannosidase from Bacteriodes thetaiotaomicron in complex with BIS-TRIS-Propane [Bacteroides thetaiotaomicron VPI-5482],4AD0_B Structure of the GH99 endo-alpha-mannosidase from Bacteriodes thetaiotaomicron in complex with BIS-TRIS-Propane [Bacteroides thetaiotaomicron VPI-5482],4AD0_C Structure of the GH99 endo-alpha-mannosidase from Bacteriodes thetaiotaomicron in complex with BIS-TRIS-Propane [Bacteroides thetaiotaomicron VPI-5482],4AD0_D Structure of the GH99 endo-alpha-mannosidase from Bacteriodes thetaiotaomicron in complex with BIS-TRIS-Propane [Bacteroides thetaiotaomicron VPI-5482]
4ACZ_B	7.00e-13	4	342	8	339	Structureof the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
4ACY_A	2.98e-12	4	342	8	339	Selenomethioninederivative of the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],4ACY_B Selenomethionine derivative of the GH99 endo-alpha-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
4AD1_A	1.68e-10	53	353	42	354	Structureof the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens [Bacteroides xylanisolvens XB1A],4AD2_A Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-isofagomine [Bacteroides xylanisolvens XB1A],4AD2_B Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-isofagomine [Bacteroides xylanisolvens XB1A],4AD2_C Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-isofagomine [Bacteroides xylanisolvens XB1A],4AD2_D Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-isofagomine [Bacteroides xylanisolvens XB1A],4AD3_A Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with Glucose-1,3-deoxymannojirimycin [Bacteroides xylanisolvens XB1A],4AD3_B Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with Glucose-1,3-deoxymannojirimycin [Bacteroides xylanisolvens XB1A],4AD3_C Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with Glucose-1,3-deoxymannojirimycin [Bacteroides xylanisolvens XB1A],4AD3_D Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with Glucose-1,3-deoxymannojirimycin [Bacteroides xylanisolvens XB1A],4AD4_A Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-isofagomine and alpha-1,2- mannobiose [Bacteroides xylanisolvens XB1A],4AD5_A Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with glucose-1,3-deoxymannojirimycin and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],4UTF_A Structure of the GH99 endo-alpha-mannosidase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-isofagomine and alpha- 1,2-mannobiose [Bacteroides xylanisolvens XB1A],4V27_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-isofagomine [Bacteroides xylanisolvens XB1A]
5LYR_A	1.71e-10	53	353	47	359	Structureof the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-noeuromycin [Bacteroides xylanisolvens XB1A],5M03_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-noeuromycin and 1,2-alpha-mannobiose [Bacteroides xylanisolvens XB1A],5M17_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-1,2-dideoxymannose [Bacteroides xylanisolvens XB1A],5M3W_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-1,2-dideoxymannose and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],5M5D_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-D-glucal [Bacteroides xylanisolvens XB1A],5MC8_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-D-glucal and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],6FAM_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-2-aminodeoxymannojirimycin [Bacteroides xylanisolvens XB1A],6FAR_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-mannoimidazole [Bacteroides xylanisolvens XB1A],6FWI_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-(1,2-anhydro-carba-mannosamine) [Bacteroides xylanisolvens XB1A],6FWJ_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-(1,2-anhydro-carba-mannosamine) and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],6FWM_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-1,2-anhydro-mannose hydrolyzed by enzyme [Bacteroides xylanisolvens XB1A],6FWP_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-1,3-mannobiose and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],6HMG_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-(1,2-anhydro-carba-glucosamine) [Bacteroides xylanisolvens XB1A],6HMH_A Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-(1,2-anhydro-carba-glucosamine) and alpha-1,2-mannobiose [Bacteroides xylanisolvens XB1A],6ZJ6_AAA Chain AAA, Glycosyl hydrolase family 71 [Bacteroides xylanisolvens XB1A]