CAZyme Information: MGYG000002596_00398

Basic Information

• Species: Mediterraneibacter sp900555215
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900555215
• CAZyme ID: MGYG000002596_00398
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
847	MGYG000002596_7|CGC1	96599.12	4.635

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002596	2662706	MAG	China	Asia

• Gene Location: Start: 14180; End: 16723 Strand: -

Full Sequence      

MTFDNMVLRASLRRCFFYALKESDWMDTLKGKQIMVWTFMGNARMYEALRDYGDRISQIG
LFSFKVRATGEIYESGVAISDMLTYINRWPHIKWLLTVANDGANSIFRALRDNTDGAQDR
FLSEIVRIMEKYPWCDGIDIDLERGDGYSTHTESTAMFCNIYNTVKAYDPAKHMNICLPG
MTSVNGSVGGENWCVYGDLDAYCDTASIMSYGMAWAGSAPGPVSPRSWLEGIYDYATKVM
NPDKIFLGMPAYGWNWQIYDTPENLGKTYRGTSNTYYAAKLWMTGGYNFTDDAPPQPFLP
IVAYWDDYDKAPYAFPHVYDYMEGADAVSREYPQLVGTYNRRRYLTAYGKEQKTEFGTVY
IDHDGGNYSSASGIVSIENGIATLGDNGSVTYSFNVDTAGTYDVAVRLCYPFWDKNGIYV
SLDGSRKHFTESRLWWPYWRSTFWTSLAENVTLSAGTHTITISVDVKGVQFYGFRVCSDF
SEEPSAGEATFALAPRSFKDVDGNMAVPDKGFKLTLEMLRRKPDSALVWYEDFRDYGVLE
TDYWTVRSGSWKVWRSDEYSMERVYSQLEGKGELAWNYDGFSELHLRARLAFPANGSGKA
GVFCGSLFCCLNYDTQAVELYSGSTLLGSYSQEITRTASADLRDNPSMYTVEMRIRGNRV
RVYSGSSYTLRFTATVSGFSGGYAGYRSDNTTVCELLRLGDAWTYEPYERFDVEMPDGSF
KSYGRISRSNCTWDEEFQVFTLTSDVEESSTRSEDISLDYDFFHSDLLEISCGGNYTARI
IPKDINIWISRLFLGDADGFSILYYQDVDSLVYWANQAAYRWKLRGMCMWSLGQEDMRLW
EWLPKQI

Enzyme Prediction     

No EC number prediction in MGYG000002596_00398.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	71	315	4e-27	0.652027027027027

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	5.74e-22	31	279	1	238	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	7.77e-15	47	274	119	342	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
cd02795	CBM6-CBM35-CBM36_like	9.94e-13	364	476	11	124	Carbohydrate Binding Module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules. This alignment model also contains the C-terminal domains of bacterial insecticidal toxins, where they may be involved in determining insect specificity through carbohydrate binding functionality.
smart00636	Glyco_18	3.77e-09	82	315	57	295	Glyco_18 domain.
pfam00704	Glyco_hydro_18	1.90e-08	90	315	62	270	Glycosyl hydrolases family 18.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFC63687.1	0.0	26	847	1	822
CBL33601.1	0.0	26	847	1	820
ADL53625.1	0.0	26	847	1	823
ACZ98659.1	0.0	26	846	1	822
QKN24290.1	0.0	26	846	3	822

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3J_A	3.36e-08	45	294	122	368	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]
4WIW_A	1.73e-06	117	307	96	294	ChainA, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_B Chain B, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_C Chain C, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_D Chain D, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_E Chain E, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_F Chain F, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0DPJ9	1.95e-08	45	294	121	367	Cortical fragment-lytic enzyme OS=Bacillus anthracis OX=1392 GN=sleL PE=2 SV=1
Q9K3E4	2.24e-06	45	294	121	367	Cortical fragment-lytic enzyme OS=Bacillus cereus OX=1396 GN=sleL PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000091	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002596_00398.